ID   TRUC_PECCC              Reviewed;         376 AA.
AC   Q47417;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=tRNA pseudouridine synthase C;
DE            EC=5.4.99.26;
DE   AltName: Full=tRNA pseudouridine(65) synthase;
DE   AltName: Full=tRNA pseudouridylate synthase C;
DE   AltName: Full=tRNA-uridine isomerase C;
GN   Name=truC;
OS   Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp.
OS   carotovora).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=555;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SCRI 193;
RA   Golby P., Jones S.E., Stephens S., Reeves P.J., Bycroft B., Stewart G.,
RA   Williams P., Salmond G.P.C.;
RT   "Global regulation of Erwinia carotovora exoenzyme virulence factors:
RT   multicopy suppression of rex mutants and evidence for a global repression
RT   regulon.";
RL   Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-65 in
CC       transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(65) in tRNA = pseudouridine(65) in tRNA;
CC         Xref=Rhea:RHEA:42536, Rhea:RHEA-COMP:10103, Rhea:RHEA-COMP:10104,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.26;
CC   -!- SIMILARITY: In the C-terminal section; belongs to the pseudouridine
CC       synthase RluA family. {ECO:0000305}.
CC   -!- SIMILARITY: To E.coli YqcC in the N-terminal section. {ECO:0000305}.
CC   -!- CAUTION: There might be a sequencing error that fuses together two
CC       ORFs. {ECO:0000305}.
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DR   EMBL; X79474; CAA55982.1; -; Genomic_DNA.
DR   PIR; S45107; S45107.
DR   AlphaFoldDB; Q47417; -.
DR   SMR; Q47417; -.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1440.40; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR023376; YqcC-like_dom.
DR   InterPro; IPR036814; YqcC-like_sf.
DR   Pfam; PF04287; DUF446; 1.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF158452; SSF158452; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
KW   Isomerase; tRNA processing.
FT   CHAIN           1..376
FT                   /note="tRNA pseudouridine synthase C"
FT                   /id="PRO_0000162724"
FT   ACT_SITE        168
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   376 AA;  43605 MW;  F35992CAAAD22E30 CRC64;
     MSRENQVRQS LFDIERALRE SPFWQVVPPE EEAFNSTEPF SLDTMKPEEW LQWVFLPRMH
     ALLDSELALP RSWFYCLILK KRWKGRQKRP RRFCCVSSSS MSYSPPMYRM AQCTMLEIIY
     QDEHLVAVNK PSGWLVHRSW LDRKEKVVVM QTVRDQIGQH VYTVHRLDRP TSGVLLLALS
     SEVARALSQQ FESHQMQKTY HAVVRGYVLD DGVIDYALTE ELDKIADKFT NPDKAPQPAV
     THYRSLAQAE MPVAIGRYPT ARYSLMELKP QTGRKHQLRR HMSHIHHPII GDTAHGDLRH
     NRGMESHFSC GRLMLHASEL QLNHPVSGQP LTLQARWDAP WQGVVTQFGW KGILPEFEGV
     EFPADSGQDS EHFVEQ