C2D1B_MOUSE
ID C2D1B_MOUSE Reviewed; 848 AA.
AC Q8BRN9; B2RQ40; Q3UJ60; Q69Z95;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Coiled-coil and C2 domain-containing protein 1B;
DE AltName: Full=Five prime repressor element under dual repression-binding protein 2;
DE Short=FRE under dual repression-binding protein 2;
DE Short=Freud-2;
GN Name=Cc2d1b; Synonyms=Kiaa1836;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain cortex, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 147-848.
RC TISSUE=Spleen;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-455; SER-583 AND THR-586, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21155902; DOI=10.1111/j.1460-9568.2010.07498.x;
RA Hadjighassem M.R., Galaraga K., Albert P.R.;
RT "Freud-2/CC2D1B mediates dual repression of the serotonin-1A receptor
RT gene.";
RL Eur. J. Neurosci. 33:214-223(2011).
CC -!- FUNCTION: Transcription factor that binds specifically to the DRE (dual
CC repressor element) and represses HTR1A gene transcription in neuronal
CC cells. {ECO:0000269|PubMed:21155902}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:21155902}.
CC -!- SIMILARITY: Belongs to the CC2D1 family. {ECO:0000305}.
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DR EMBL; AK043839; BAC31675.1; -; mRNA.
DR EMBL; AK146601; BAE27295.1; -; mRNA.
DR EMBL; AL626783; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC125517; AAI25518.1; -; mRNA.
DR EMBL; BC137743; AAI37744.1; -; mRNA.
DR EMBL; AK173271; BAD32549.1; -; mRNA.
DR CCDS; CCDS18454.1; -.
DR RefSeq; NP_796019.1; NM_177045.3.
DR AlphaFoldDB; Q8BRN9; -.
DR SMR; Q8BRN9; -.
DR BioGRID; 235657; 2.
DR STRING; 10090.ENSMUSP00000030320; -.
DR iPTMnet; Q8BRN9; -.
DR PhosphoSitePlus; Q8BRN9; -.
DR EPD; Q8BRN9; -.
DR jPOST; Q8BRN9; -.
DR MaxQB; Q8BRN9; -.
DR PaxDb; Q8BRN9; -.
DR PeptideAtlas; Q8BRN9; -.
DR PRIDE; Q8BRN9; -.
DR ProteomicsDB; 273809; -.
DR Antibodypedia; 33001; 64 antibodies from 22 providers.
DR Ensembl; ENSMUST00000030320; ENSMUSP00000030320; ENSMUSG00000028582.
DR GeneID; 319965; -.
DR KEGG; mmu:319965; -.
DR UCSC; uc008ubl.1; mouse.
DR CTD; 200014; -.
DR MGI; MGI:2443076; Cc2d1b.
DR VEuPathDB; HostDB:ENSMUSG00000028582; -.
DR eggNOG; KOG3837; Eukaryota.
DR GeneTree; ENSGT00390000009595; -.
DR InParanoid; Q8BRN9; -.
DR OMA; KMADECM; -.
DR OrthoDB; 959801at2759; -.
DR PhylomeDB; Q8BRN9; -.
DR TreeFam; TF314229; -.
DR Reactome; R-MMU-9668328; Sealing of the nuclear envelope (NE) by ESCRT-III.
DR BioGRID-ORCS; 319965; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Cc2d1b; mouse.
DR PRO; PR:Q8BRN9; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8BRN9; protein.
DR Bgee; ENSMUSG00000028582; Expressed in humerus cartilage element and 227 other tissues.
DR ExpressionAtlas; Q8BRN9; baseline and differential.
DR Genevisible; Q8BRN9; MM.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd08690; C2_Freud-1; 1.
DR Gene3D; 2.60.40.150; -; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037772; C2_Freud.
DR InterPro; IPR039725; CC2D1A/B.
DR InterPro; IPR006608; DM14.
DR PANTHER; PTHR13076; PTHR13076; 1.
DR Pfam; PF00168; C2; 1.
DR SMART; SM00239; C2; 1.
DR SMART; SM00685; DM14; 4.
DR SUPFAM; SSF49562; SSF49562; 1.
DR PROSITE; PS50004; C2; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..848
FT /note="Coiled-coil and C2 domain-containing protein 1B"
FT /id="PRO_0000288427"
FT DOMAIN 666..805
FT /note="C2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 53..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 217..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 326..353
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 433..460
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 476..523
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 165..193
FT /evidence="ECO:0000255"
FT COILED 600..626
FT /evidence="ECO:0000255"
FT COMPBIAS 501..523
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 455
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 583
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 586
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 25
FT /note="L -> F (in Ref. 1; BAE27295)"
FT /evidence="ECO:0000305"
FT CONFLICT 388
FT /note="Q -> L (in Ref. 1; BAE27295)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 848 AA; 93091 MW; 32F781AF50CA8297 CRC64;
MPGPRPRKGP KTSGQGAETA KQLGLFVEFN PEDMLLGVDE TEDDGDLEAE LLALTGETAS
RSRKPAPKGQ APLPMAHIEK LAADCMRDVE EDEEEEGLED DADLLTELQE VLGEDEEAGL
LDGSEAASPD LCEEKTWDNT ELPVEQAACQ QAVPAAAQAG GPRGLQALLE ERIRNYREAA
ASAKEAGEAA KARRCERGLK TLQSQLATVR KGGKICEDEI PPPVALGKRP PAPQERAIKN
PEIDSPGPCA MEPGNLSQPE SSLPAIAPLP DSDPDPQALL LARQREYKAA ALDAKRAGDL
DRARELMRIG KRFGTVLEAL EKGQPVDLSG MPPAPADLKA LPQASKASSA TQGLSPAVEQ
MQPVMASDLP ATPVAPAEPT TVLDALQQRL NKYREAGIQA RANGDERKAR MHDRIAKQYQ
DAVRAHQAGQ KVDFAELPVP PGFPPIPGLE PRKGSEQDSV AATLATAQKL ASEDAALVDD
DEESDTPAQA PLAKKPAQTL VSPSHLLTEP KASSSKESLS PSVREQVTLL EARKLQYQRA
ALQAKRRQDL EQAKSHLRVA KSLEAQIIQA RAGQPIDLSK VPSPLTDEEG DFILIHHEDL
RLSQKAEEVY AQLQKMLQEQ QAKCLLFSKQ YMHQGNVAET TRFERLAEDR KKQLEILQLA
QAQGLDPPSH HFELKTFQTV RIFSELNSTE MHLIIVRGMN LPAPPGVTPD DLDAFVRFEF
HYPNSDQAQK SKTAVVKNTN SPEFEQVFKL NINRNHRGFR RVIQSKGIKF EIFHKGSFFR
SDKLVGTAHL KLERLEKECE IREIMEVLDG RKPTGGKLEV KVRLREPLSS QDVQTVTENW
LVLEPRGL
