ID   TRUC_SHIFL              Reviewed;         260 AA.
AC   P0AA42; Q46918;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=tRNA pseudouridine synthase C;
DE            EC=5.4.99.26;
DE   AltName: Full=tRNA pseudouridine(65) synthase;
DE   AltName: Full=tRNA pseudouridylate synthase C;
DE   AltName: Full=tRNA-uridine isomerase C;
GN   Name=truC; OrderedLocusNames=SF2804, S2998;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-65 in
CC       transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(65) in tRNA = pseudouridine(65) in tRNA;
CC         Xref=Rhea:RHEA:42536, Rhea:RHEA-COMP:10103, Rhea:RHEA-COMP:10104,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.26;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE005674; AAN44292.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP18117.1; -; Genomic_DNA.
DR   RefSeq; NP_708585.1; NC_004337.2.
DR   RefSeq; WP_000890001.1; NZ_WPGW01000063.1.
DR   AlphaFoldDB; P0AA42; -.
DR   SMR; P0AA42; -.
DR   STRING; 198214.SF2804; -.
DR   EnsemblBacteria; AAN44292; AAN44292; SF2804.
DR   EnsemblBacteria; AAP18117; AAP18117; S2998.
DR   GeneID; 1026915; -.
DR   GeneID; 67413934; -.
DR   KEGG; sfl:SF2804; -.
DR   KEGG; sfx:S2998; -.
DR   PATRIC; fig|198214.7.peg.3338; -.
DR   HOGENOM; CLU_016902_11_4_6; -.
DR   OMA; DRHETQF; -.
DR   OrthoDB; 1714699at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..260
FT                   /note="tRNA pseudouridine synthase C"
FT                   /id="PRO_0000162719"
FT   ACT_SITE        54
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   260 AA;  29666 MW;  0BEA421482CDB89A CRC64;
     MLEILYQDEW LVAVNKPSGW LVHRSWLDRD EKVVVMQTVR DQIGQHVFTA HRLDRPTSGV
     LLMGLSSEAG RLLAQQFEQH QIQKRYHAIV RGWLMEEAVL DYPLVEELDK IADKFAREDK
     GPQPAVTHYR GLATVEMPVA TGRYPTTRYG LVELEPKTGR KHQLRRHLAH LRHPIIGDSK
     HGDLRQNRSG AEHFGLQRLM LHASQLSLTH PFTGEPLTIH AGLDDTWMQA LSQFGWRGLL
     PENERVEFSA PSGQDGEISS