ID   TRUC_VIBCH              Reviewed;         244 AA.
AC   Q9KTL4;
DT   09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=tRNA pseudouridine synthase C;
DE            EC=5.4.99.26;
DE   AltName: Full=tRNA pseudouridine(65) synthase;
DE   AltName: Full=tRNA pseudouridylate synthase C;
DE   AltName: Full=tRNA-uridine isomerase C;
GN   Name=truC; OrderedLocusNames=VC_0888;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-65 in
CC       transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(65) in tRNA = pseudouridine(65) in tRNA;
CC         Xref=Rhea:RHEA:42536, Rhea:RHEA-COMP:10103, Rhea:RHEA-COMP:10104,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.26;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE003852; AAF94050.1; -; Genomic_DNA.
DR   PIR; G82266; G82266.
DR   RefSeq; NP_230535.1; NC_002505.1.
DR   RefSeq; WP_000890132.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9KTL4; -.
DR   SMR; Q9KTL4; -.
DR   STRING; 243277.VC_0888; -.
DR   DNASU; 2614117; -.
DR   EnsemblBacteria; AAF94050; AAF94050; VC_0888.
DR   GeneID; 57739581; -.
DR   KEGG; vch:VC_0888; -.
DR   PATRIC; fig|243277.26.peg.845; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_11_4_6; -.
DR   OMA; DRHETQF; -.
DR   BioCyc; VCHO:VC0888-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..244
FT                   /note="tRNA pseudouridine synthase C"
FT                   /id="PRO_0000162720"
FT   ACT_SITE        54
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   244 AA;  28232 MW;  71A8BB952CE28C75 CRC64;
     MLEIVYQDEY LVAVNKPAGM LVHRSWLDKH ETEFVMQTLR DQIGQHVFPL HRLDRPTSGV
     LVFALSSQIA SEVMPMFANH EMEKTYHAIV RGWIEEANVL DYPLKEELDK IADKFAKQDK
     EAQSAVTAYR PLAKVELPIA TGKFATTRYC LMEMQPKTGR KHQLRRHMAH LRHPIVGDTT
     HGDGVHNRLF REHFSAQRLM LHASELRFMH PYTQQPLVIR AGLDEVWQGL LSEFGWQESL
     LINA