ID   TRUC_VIBPA              Reviewed;         246 AA.
AC   Q87MD4;
DT   09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=tRNA pseudouridine synthase C;
DE            EC=5.4.99.26;
DE   AltName: Full=tRNA pseudouridine(65) synthase;
DE   AltName: Full=tRNA pseudouridylate synthase C;
DE   AltName: Full=tRNA-uridine isomerase C;
GN   Name=truC; OrderedLocusNames=VP2322;
OS   Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=223926;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RIMD 2210633;
RX   PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA   Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA   Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA   Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT   "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT   distinct from that of V. cholerae.";
RL   Lancet 361:743-749(2003).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-65 in
CC       transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(65) in tRNA = pseudouridine(65) in tRNA;
CC         Xref=Rhea:RHEA:42536, Rhea:RHEA-COMP:10103, Rhea:RHEA-COMP:10104,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.26;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; BA000031; BAC60585.1; -; Genomic_DNA.
DR   RefSeq; NP_798701.1; NC_004603.1.
DR   AlphaFoldDB; Q87MD4; -.
DR   SMR; Q87MD4; -.
DR   STRING; 223926.28807320; -.
DR   PRIDE; Q87MD4; -.
DR   EnsemblBacteria; BAC60585; BAC60585; BAC60585.
DR   KEGG; vpa:VP2322; -.
DR   PATRIC; fig|223926.6.peg.2224; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_11_4_6; -.
DR   OMA; DRHETQF; -.
DR   Proteomes; UP000002493; Chromosome 1.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..246
FT                   /note="tRNA pseudouridine synthase C"
FT                   /id="PRO_0000162721"
FT   ACT_SITE        58
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   246 AA;  28712 MW;  89AF262B22FD4E26 CRC64;
     MAPVELEIVY QDEYFVAVNK PAGMLVHRSW LDKHETQFVM QTLRDQIGQH VFPLHRLDRP
     TSGVLVFALS SEVASQVMPM FAEHKMEKTY HAIVRGWIEE EGVLDYALKV ELDKIADKFA
     SQEKEAQEAV TAYKPLAKVE VPYSTGKFPT TRYCLMEMKP KTGRKHQLRR HMAHLRHPIV
     GDTSHGDGKH NKLFRNEFDS HRLLLHASEL RFVHPFTNEE LVMKASIDDT WQQLFTRFEW
     DEELVK