ID   TRUD_AQUAE              Reviewed;         385 AA.
AC   O67616;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=tRNA pseudouridine synthase D {ECO:0000255|HAMAP-Rule:MF_01082};
DE            EC=5.4.99.27 {ECO:0000255|HAMAP-Rule:MF_01082};
DE   AltName: Full=tRNA pseudouridine(13) synthase {ECO:0000255|HAMAP-Rule:MF_01082};
DE   AltName: Full=tRNA pseudouridylate synthase D {ECO:0000255|HAMAP-Rule:MF_01082};
DE   AltName: Full=tRNA-uridine isomerase D {ECO:0000255|HAMAP-Rule:MF_01082};
GN   Name=truD {ECO:0000255|HAMAP-Rule:MF_01082}; OrderedLocusNames=aq_1723;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-13 in
CC       transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01082}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(13) in tRNA = pseudouridine(13) in tRNA;
CC         Xref=Rhea:RHEA:42540, Rhea:RHEA-COMP:10105, Rhea:RHEA-COMP:10106,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.27;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01082};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family.
CC       {ECO:0000255|HAMAP-Rule:MF_01082}.
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DR   EMBL; AE000657; AAC07586.1; -; Genomic_DNA.
DR   PIR; F70448; F70448.
DR   RefSeq; NP_214182.1; NC_000918.1.
DR   RefSeq; WP_010881119.1; NC_000918.1.
DR   AlphaFoldDB; O67616; -.
DR   SMR; O67616; -.
DR   STRING; 224324.aq_1723; -.
DR   PRIDE; O67616; -.
DR   EnsemblBacteria; AAC07586; AAC07586; aq_1723.
DR   KEGG; aae:aq_1723; -.
DR   PATRIC; fig|224324.8.peg.1324; -.
DR   eggNOG; COG0585; Bacteria.
DR   HOGENOM; CLU_005281_4_1_0; -.
DR   InParanoid; O67616; -.
DR   OMA; GFPNYFD; -.
DR   OrthoDB; 1490777at2; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.2350.20; -; 2.
DR   HAMAP; MF_01082; TruD; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001656; PsdUridine_synth_TruD.
DR   InterPro; IPR020119; PsdUridine_synth_TruD_CS.
DR   InterPro; IPR011760; PsdUridine_synth_TruD_insert.
DR   InterPro; IPR042214; TruD_catalytic.
DR   PANTHER; PTHR13326; PTHR13326; 1.
DR   Pfam; PF01142; TruD; 1.
DR   PIRSF; PIRSF037016; Pseudouridin_synth_euk_prd; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00094; tRNA_TruD_broad; 1.
DR   PROSITE; PS50984; TRUD; 1.
DR   PROSITE; PS01268; UPF0024; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..385
FT                   /note="tRNA pseudouridine synthase D"
FT                   /id="PRO_0000152491"
FT   DOMAIN          143..345
FT                   /note="TRUD"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01082"
FT   ACT_SITE        65
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01082"
SQ   SEQUENCE   385 AA;  45775 MW;  84DCA37687111833 CRC64;
     MDIRIKEKPE EFYVKEIKKL DLKEKGQYAY FLLKKKDMTT LDAVRHISHR FGIPLKNIGF
     AGLKDKKAVT EQYISVKDLN EEKIRKMDGY RTENLELKFL GFSDKGLELG EIEGNYFEVV
     VRGVTKYHRR VFPRMKELVE NYGCENYFGE QRFGSVKHAE EFIVKYLLRH EYEEAMKEYL
     TSLGDKRLKR LLRKAWRDWD RFLSLMPKGA KPELEVVKAL RRGESFKNAF MVLPKNIRLM
     FVFAYQSYLW NRYLYTFVVR YLKYCKTPFL KWELAFFNDM SEVIWEEIKD LEIPYLGVEY
     KPRNKKAEIV MKEVLQDEGI TPKMLMAERI GIKLFSDGVR KAFFKPQGLK VIEERKNSLK
     LSFTLPPGSY ATILLRKLFC SDVKS