ID   TRUD_CAMJE              Reviewed;         372 AA.
AC   Q9PMK3; Q0P8F8;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=tRNA pseudouridine synthase D {ECO:0000255|HAMAP-Rule:MF_01082};
DE            EC=5.4.99.27 {ECO:0000255|HAMAP-Rule:MF_01082};
DE   AltName: Full=tRNA pseudouridine(13) synthase {ECO:0000255|HAMAP-Rule:MF_01082};
DE   AltName: Full=tRNA pseudouridylate synthase D {ECO:0000255|HAMAP-Rule:MF_01082};
DE   AltName: Full=tRNA-uridine isomerase D {ECO:0000255|HAMAP-Rule:MF_01082};
GN   Name=truD {ECO:0000255|HAMAP-Rule:MF_01082}; OrderedLocusNames=Cj1457c;
OS   Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC
OS   11168).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=192222;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700819 / NCTC 11168;
RX   PubMed=10688204; DOI=10.1038/35001088;
RA   Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M.,
RA   Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S.,
RA   Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A.,
RA   Rajandream M.A., Rutherford K.M., van Vliet A.H.M., Whitehead S.,
RA   Barrell B.G.;
RT   "The genome sequence of the food-borne pathogen Campylobacter jejuni
RT   reveals hypervariable sequences.";
RL   Nature 403:665-668(2000).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-13 in
CC       transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01082}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(13) in tRNA = pseudouridine(13) in tRNA;
CC         Xref=Rhea:RHEA:42540, Rhea:RHEA-COMP:10105, Rhea:RHEA-COMP:10106,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.27;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01082};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family.
CC       {ECO:0000255|HAMAP-Rule:MF_01082}.
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DR   EMBL; AL111168; CAL35565.1; -; Genomic_DNA.
DR   PIR; H81291; H81291.
DR   RefSeq; WP_002851259.1; NC_002163.1.
DR   RefSeq; YP_002344839.1; NC_002163.1.
DR   AlphaFoldDB; Q9PMK3; -.
DR   SMR; Q9PMK3; -.
DR   IntAct; Q9PMK3; 33.
DR   STRING; 192222.Cj1457c; -.
DR   PaxDb; Q9PMK3; -.
DR   PRIDE; Q9PMK3; -.
DR   EnsemblBacteria; CAL35565; CAL35565; Cj1457c.
DR   GeneID; 905745; -.
DR   KEGG; cje:Cj1457c; -.
DR   PATRIC; fig|192222.6.peg.1437; -.
DR   eggNOG; COG0585; Bacteria.
DR   HOGENOM; CLU_005281_4_0_7; -.
DR   OMA; LWLWVEK; -.
DR   Proteomes; UP000000799; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.2350.20; -; 1.
DR   HAMAP; MF_01082; TruD; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001656; PsdUridine_synth_TruD.
DR   InterPro; IPR020119; PsdUridine_synth_TruD_CS.
DR   InterPro; IPR011760; PsdUridine_synth_TruD_insert.
DR   InterPro; IPR042214; TruD_catalytic.
DR   Pfam; PF01142; TruD; 2.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00094; tRNA_TruD_broad; 1.
DR   PROSITE; PS50984; TRUD; 1.
DR   PROSITE; PS01268; UPF0024; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..372
FT                   /note="tRNA pseudouridine synthase D"
FT                   /id="PRO_0000152492"
FT   DOMAIN          160..330
FT                   /note="TRUD"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01082"
FT   ACT_SITE        85
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01082"
SQ   SEQUENCE   372 AA;  43445 MW;  BDA9F771F0A2D3C7 CRC64;
     MNLEEENTIF KPLYSLKHSP INAYFSKNSD DFVVRERPLY EFSGKGEHLI LHINKKDLTT
     NEALKILSEA SGVKIRDFGY AGLKDKQGST FQYLSMPKKF ESFLSNFSHP KLKILEIFTH
     ENKLRIGHLK GNSFFIRLKK VLPSDALKLE QALMNLDKQG FANYFGYQRF GKFGDNYKEG
     LEILRGKKMK NVKMKEFLIS AFQSELFNRY LSKRVELSHF ANDFSEKELI QIYKISKEEA
     KELKKQEQFF KLLKGEVLGH YPFGKCFLCE DLSAELGRFK ARDISAMGLL IGAKAYETGE
     GLALNLENEI FKDTLEFKAK MQGSRRFMWG YLEELKWRYD EEKAHFCIEF FLQKGSYATV
     VLEEILHKNL FE