C2D1_DROME
ID C2D1_DROME Reviewed; 816 AA.
AC Q9VKJ9;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Coiled-coil and C2 domain-containing protein 1-like;
DE AltName: Full=Lethal giant disks protein;
GN Name=l(2)gd1; Synonyms=lgd; ORFNames=CG4713;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=12648493; DOI=10.1016/s0012-1606(02)00052-0;
RA Klein T.;
RT "The tumour suppressor gene l(2)giant discs is required to restrict the
RT activity of Notch to the dorsoventral boundary during Drosophila wing
RT development.";
RL Dev. Biol. 255:313-333(2003).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=17088062; DOI=10.1016/j.cub.2006.09.031;
RA Childress J.L., Acar M., Tao C., Halder G.;
RT "Lethal giant discs, a novel C2-domain protein, restricts notch activation
RT during endocytosis.";
RL Curr. Biol. 16:2228-2233(2006).
CC -!- FUNCTION: Negative regulator of the Notch signaling pathway, acting to
CC restrict the activity of Notch to the dorsoventral (D/V) boundary of
CC the wing imaginal disk. Also causes negative regulation of Notch during
CC vein, eye, and bristle development. Acts by targeting Notch for
CC endosomal degradation or recycling. {ECO:0000269|PubMed:12648493,
CC ECO:0000269|PubMed:17088062}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17088062}.
CC -!- TISSUE SPECIFICITY: Expressed in wing and eye imaginal disks.
CC {ECO:0000269|PubMed:12648493, ECO:0000269|PubMed:17088062}.
CC -!- DISRUPTION PHENOTYPE: Flies exhibit problems in vein, eye, and bristle
CC development. {ECO:0000269|PubMed:12648493}.
CC -!- SIMILARITY: Belongs to the CC2D1 family. {ECO:0000305}.
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DR EMBL; AE014134; AAF53069.1; -; Genomic_DNA.
DR EMBL; AY058523; AAL13752.1; -; mRNA.
DR RefSeq; NP_609488.1; NM_135644.4.
DR PDB; 5VNY; X-ray; 1.10 A; A=359-423.
DR PDB; 5VO5; X-ray; 2.00 A; A=359-423.
DR PDB; 6EI6; X-ray; 2.46 A; A/B=550-816.
DR PDBsum; 5VNY; -.
DR PDBsum; 5VO5; -.
DR PDBsum; 6EI6; -.
DR AlphaFoldDB; Q9VKJ9; -.
DR SMR; Q9VKJ9; -.
DR BioGRID; 60605; 16.
DR IntAct; Q9VKJ9; 12.
DR STRING; 7227.FBpp0297275; -.
DR PaxDb; Q9VKJ9; -.
DR DNASU; 34543; -.
DR EnsemblMetazoa; FBtr0080197; FBpp0079786; FBgn0261983.
DR GeneID; 34543; -.
DR KEGG; dme:Dmel_CG4713; -.
DR FlyBase; FBgn0261983; l(2)gd1.
DR VEuPathDB; VectorBase:FBgn0261983; -.
DR eggNOG; KOG3837; Eukaryota.
DR GeneTree; ENSGT00390000009595; -.
DR InParanoid; Q9VKJ9; -.
DR OMA; NLAKQHY; -.
DR PhylomeDB; Q9VKJ9; -.
DR Reactome; R-DME-9668328; Sealing of the nuclear envelope (NE) by ESCRT-III.
DR BioGRID-ORCS; 34543; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 34543; -.
DR PRO; PR:Q9VKJ9; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0261983; Expressed in embryonic/larval hemocyte (Drosophila) and 25 other tissues.
DR ExpressionAtlas; Q9VKJ9; baseline and differential.
DR Genevisible; Q9VKJ9; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0098592; C:cytoplasmic side of apical plasma membrane; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0005543; F:phospholipid binding; IDA:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048749; P:compound eye development; IMP:FlyBase.
DR GO; GO:0016197; P:endosomal transport; IMP:FlyBase.
DR GO; GO:0048132; P:female germ-line stem cell asymmetric division; IMP:FlyBase.
DR GO; GO:0008586; P:imaginal disc-derived wing vein morphogenesis; IMP:FlyBase.
DR GO; GO:0006886; P:intracellular protein transport; IMP:FlyBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:FlyBase.
DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IMP:FlyBase.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007423; P:sensory organ development; IMP:FlyBase.
DR GO; GO:0045035; P:sensory organ precursor cell division; IMP:FlyBase.
DR GO; GO:0007472; P:wing disc morphogenesis; IMP:FlyBase.
DR CDD; cd08690; C2_Freud-1; 1.
DR Gene3D; 2.60.40.150; -; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037772; C2_Freud.
DR InterPro; IPR039725; CC2D1A/B.
DR InterPro; IPR006608; DM14.
DR PANTHER; PTHR13076; PTHR13076; 1.
DR Pfam; PF00168; C2; 1.
DR SMART; SM00239; C2; 1.
DR SMART; SM00685; DM14; 4.
DR SUPFAM; SSF49562; SSF49562; 1.
DR PROSITE; PS50004; C2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Cytoplasm; Notch signaling pathway;
KW Reference proteome.
FT CHAIN 1..816
FT /note="Coiled-coil and C2 domain-containing protein 1-like"
FT /id="PRO_0000288430"
FT DOMAIN 637..776
FT /note="C2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT REGION 1..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 157..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 185..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 418..492
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 354..385
FT /evidence="ECO:0000255"
FT COMPBIAS 1..18
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 31..45
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 84..98
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 211..253
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 254..269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 418..447
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 448..468
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 361..380
FT /evidence="ECO:0007829|PDB:5VNY"
FT TURN 381..383
FT /evidence="ECO:0007829|PDB:5VNY"
FT HELIX 385..406
FT /evidence="ECO:0007829|PDB:5VNY"
FT HELIX 413..415
FT /evidence="ECO:0007829|PDB:5VNY"
FT HELIX 580..608
FT /evidence="ECO:0007829|PDB:6EI6"
FT HELIX 612..637
FT /evidence="ECO:0007829|PDB:6EI6"
FT STRAND 645..648
FT /evidence="ECO:0007829|PDB:6EI6"
FT HELIX 654..656
FT /evidence="ECO:0007829|PDB:6EI6"
FT STRAND 665..674
FT /evidence="ECO:0007829|PDB:6EI6"
FT STRAND 686..691
FT /evidence="ECO:0007829|PDB:6EI6"
FT STRAND 695..697
FT /evidence="ECO:0007829|PDB:6EI6"
FT STRAND 701..703
FT /evidence="ECO:0007829|PDB:6EI6"
FT STRAND 717..722
FT /evidence="ECO:0007829|PDB:6EI6"
FT HELIX 728..736
FT /evidence="ECO:0007829|PDB:6EI6"
FT STRAND 738..745
FT /evidence="ECO:0007829|PDB:6EI6"
FT STRAND 754..763
FT /evidence="ECO:0007829|PDB:6EI6"
FT HELIX 764..767
FT /evidence="ECO:0007829|PDB:6EI6"
FT STRAND 770..797
FT /evidence="ECO:0007829|PDB:6EI6"
FT HELIX 801..809
FT /evidence="ECO:0007829|PDB:6EI6"
FT STRAND 811..814
FT /evidence="ECO:0007829|PDB:6EI6"
SQ SEQUENCE 816 AA; 89038 MW; CD757E267DA491E5 CRC64;
MFSRKKPEPA KRRQHDLSQF GLTEIPDDFD PSAGYGEDDG GDSDLEAELA AITGGEGAKP
KPKPKAKLLP ASDLDKMIAD SLRDVSDDDD DDNLESDPDL LGELSGIGGL EEAEEEEPVA
QPPAASEEPV QTFLPTTTVD TLSIIKQRLE MYKQAEANAK TAGDSGKARR FGRGLKTLKD
LHRQAAAGKS INVDDIPPEV SVKPIGGQAP PVPAEESPAP STPASPPPVP SRAAPDPPTP
GTPVEPTTSV APTSPPNPLV TQMRSRQTDY KAAALQSKRS GDISTALQFL KVVKQFDVVI
KMCEDGQEVD LSDMPPPPAE FLEFLKKMQE EAAAEAVAEP TAAPEPTPVA PAPVLAAATN
MLEALQQRLE KYQSVEAAAK AENNSGKARR FGRIVKQYED AIKLYKAGKP VPYDELPVPP
GFGPLPTADA APVAPTPSLP TSPTSPPPTA STSAGGTPSS SSATTPTAPR KAPSPPKPKE
LTTRTSGNQQ KNNIAEQQMK LLLERQKEFK LAAIEAKKAG EIDQAKEYLK IFKGFDSLLN
AASSGLPVDL STLPVPPSQR DNLEASFAIV SAEECDPTDD ICEIGVRMEE QLAKQLMMCK
NTRDHHKAMG DVAGMNRFEN LALTVQKDLD LVRYSKRKNE PLPKFHYEKR SFNIVHCNTD
LTDSELEIVV VRGISYNVAN PKDVDTYVRV EFPLLNDESF KTKTNVIRDT SSPDYDERFK
VDIQRTNRQF QRIFKRHGVK FEIYSRGGFL RSDTLIGTVN VKLQPLETKC EIHDTYDLMD
GRKQVGGKLE VKIRVRNPIL TKQMEHITEK WLVLDA
