C2D2A_DROME
ID C2D2A_DROME Reviewed; 1336 AA.
AC A1ZAV1; D6W4W4; Q8MRX3;
DT 05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-2015, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Coiled-coil and C2 domain-containing protein 2A {ECO:0000312|FlyBase:FBgn0263113};
GN Name=Cc2d2a {ECO:0000312|FlyBase:FBgn0263113};
GN ORFNames=CG43370 {ECO:0000312|FlyBase:FBgn0263113};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAM51076.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 818-1336.
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM51076.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAM51076.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000312|EMBL:ADI46810.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 407-817.
RC STRAIN=Berkeley {ECO:0000312|EMBL:ADI46810.1};
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (JUN-2010) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN THE MKS COMPLEX, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RX PubMed=27646273; DOI=10.1083/jcb.201603086;
RA Vieillard J., Paschaki M., Duteyrat J.L., Augiere C., Cortier E.,
RA Lapart J.A., Thomas J., Durand B.;
RT "Transition zone assembly and its contribution to axoneme formation in
RT Drosophila male germ cells.";
RL J. Cell Biol. 214:875-889(2016).
RN [6] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN THE MKS COMPLEX, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RX PubMed=27577095; DOI=10.1242/jcs.194621;
RA Pratt M.B., Titlow J.S., Davis I., Barker A.R., Dawe H.R., Raff J.W.,
RA Roque H.;
RT "Drosophila sensory cilia lacking MKS proteins exhibit striking defects in
RT development but only subtle defects in adults.";
RL J. Cell Sci. 129:3732-3743(2016).
CC -!- FUNCTION: Probable component of the tectonic-like complex (also named
CC MKS complex), a complex localized at the transition zone of primary
CC cilia (Probable). Required for ciliary structure and function (By
CC similarity). {ECO:0000250|UniProtKB:Q8CFW7,
CC ECO:0000305|PubMed:27577095, ECO:0000305|PubMed:27646273}.
CC -!- SUBUNIT: Probable component of the tectonic-like complex (also named
CC MKS complex), composed of B9d1, B9d2, Cc2d2a, Mks1 and tctn.
CC {ECO:0000305|PubMed:27577095, ECO:0000305|PubMed:27646273}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body
CC {ECO:0000269|PubMed:27577095, ECO:0000269|PubMed:27646273}. Cytoplasm,
CC cytoskeleton, microtubule organizing center, centrosome, centriole
CC {ECO:0000269|PubMed:27646273}. Note=Localizes at the transition zone
CC (TZ), a region between the basal body and the ciliary axoneme
CC (PubMed:27646273, PubMed:27577095). Co-localizes with the tectonic-like
CC complex (PubMed:27646273). {ECO:0000269|PubMed:27577095,
CC ECO:0000269|PubMed:27646273}.
CC -!- TISSUE SPECIFICITY: Expressed in the antennae of chordotonal neurons
CC and male germ cells (at protein level). {ECO:0000269|PubMed:27577095,
CC ECO:0000269|PubMed:27646273}.
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DR EMBL; AE013599; AAF57841.2; -; Genomic_DNA.
DR EMBL; AY119216; AAM51076.1; -; mRNA.
DR EMBL; BT125014; ADI46810.1; -; mRNA.
DR RefSeq; NP_611229.2; NM_137385.2.
DR AlphaFoldDB; A1ZAV1; -.
DR STRING; 7227.FBpp0298338; -.
DR PaxDb; A1ZAV1; -.
DR EnsemblMetazoa; FBtr0307337; FBpp0298338; FBgn0263113.
DR GeneID; 36982; -.
DR KEGG; dme:Dmel_CG43370; -.
DR UCSC; CG18432-RA; d. melanogaster.
DR CTD; 57545; -.
DR FlyBase; FBgn0263113; Cc2d2a.
DR VEuPathDB; VectorBase:FBgn0263113; -.
DR eggNOG; KOG3639; Eukaryota.
DR GeneTree; ENSGT00940000167943; -.
DR HOGENOM; CLU_001707_0_0_1; -.
DR InParanoid; A1ZAV1; -.
DR OMA; VISVWVF; -.
DR OrthoDB; 246535at2759; -.
DR PhylomeDB; A1ZAV1; -.
DR BioGRID-ORCS; 36982; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36982; -.
DR PRO; PR:A1ZAV1; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0263113; Expressed in testis and 2 other tissues.
DR Genevisible; A1ZAV1; DM.
DR GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
DR GO; GO:0035869; C:ciliary transition zone; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0036038; C:MKS complex; ISS:FlyBase.
DR GO; GO:0060271; P:cilium assembly; ISM:FlyBase.
DR GO; GO:1905515; P:non-motile cilium assembly; IBA:GO_Central.
DR GO; GO:1904491; P:protein localization to ciliary transition zone; IBA:GO_Central.
DR Gene3D; 2.60.40.150; -; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR028928; CC2D2AN-C2.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF15625; CC2D2AN-C2; 1.
DR SMART; SM00239; C2; 1.
DR SUPFAM; SSF49562; SSF49562; 1.
DR PROSITE; PS50004; C2; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium biogenesis/degradation; Coiled coil; Cytoplasm;
KW Cytoskeleton; Reference proteome.
FT CHAIN 1..1336
FT /note="Coiled-coil and C2 domain-containing protein 2A"
FT /id="PRO_0000445803"
FT DOMAIN 755..915
FT /note="C2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 70..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 136..156
FT /evidence="ECO:0000255"
FT COMPBIAS 70..85
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 997
FT /note="Q -> R (in Ref. 3; AAM51076)"
FT /evidence="ECO:0000305"
FT CONFLICT 1158
FT /note="Q -> H (in Ref. 3; AAM51076)"
FT /evidence="ECO:0000305"
FT CONFLICT 1181..1185
FT /note="RRRVP -> SRRVT (in Ref. 3; AAM51076)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1336 AA; 154424 MW; FC5DCBDF4E155820 CRC64;
MEAALSRKTA KKKRKTHTTR GYRKREQEVQ KLIRATEGRI SGPEHDMEAL SLQFFHGFEG
EMQQENDFAV EDCQESDEDS GGELAEEPTD SPSQTFIHID LHDPPLAWGQ LFKQRHYYDE
SLLYRPSNTL HFQLHLSDLS ELKDSQIRML NRYQEQSKSK SGKLGSDLIG QLSGQKPASF
LMGKHLYRSV CHRRFQSVFF PPMPPSELKG LPSRRCLKLC LKQLRFSLHP QLLEEHRLAQ
QLEDLFDVYS QQRKQRICRK LREELEIARQ VALKLLASAG QDQTAEVKRQ LKLTRQLRQR
YYAESAAQRN LLQRLLKEWA KLKELRRQQR FQCTRFQLGL RVVHPPDLEA SYCAWKESFE
TDLAEVYREH LELFYTRLRL WTDQKSHSRT ATGHSKPPRK PQFDRIMASL RKEYDKTFKD
PEEPYVEVFR LHADEATARL SIPGGDQLPK ARNYFLKIFL DGQFVGQSRT YRLEPDLQIS
INECIGVLLE RSLPENLNIW LYEKSTLTPK SRRLAQISTP LVLSSKDDAV QEKLSFQALS
STQLAGDVYL YYEYRSTEGW GSALHLDDVQ RLPDALRQTL LPQSLPALPQ ASKELVTPRP
PSGRIKKSQL PSLIFAEQQL QFCGLDVLLD NRRFQLLHSR HQQRNLHTKQ LRFVPALEQE
ISEEEPLPDG RGTVQLLEPG TYWNPIDLHK HRGRKFLQLL YEVIASQSAR RAKALQTPLP
LLLLAEDSDR SSGTGWTALW RAFCSVFQGQ RNSLPREPSG WSGHQSAPDL FKSFCVSLHV
VRATGVPVRS RHILNLNERR SSAGGDMSTS LFVTQTLMYS NVRPFVTLSY GQRLCRSRTA
EGSNPTWNEQ LQLQIQSQFG DLRDDLKISL FDELIEQQYS DEASDVYQRV QCNWLGEFRV
PINSLLASRT FEGCIELAMP KVLVGYKRPL IDSVTNMPTD QYPEFKEAVH LWFYLSIEPG
GGDLAPLHSC ALACAEKPEL QQYLDERRLE LQQLLPQPQR YVEPLVCTAQ GKRVCLTRLM
DPVPLPPAAV PSGENPLEIC IRYVSLLSHL RSYDPCQGFR GVWLDNQSLL DSTWCSVKDL
GVLLCNYMLS LGLECWLILG VSCPHGECSF VLFRQPETAE LFLVSPATGK RYQLQDVHCP
LRRIYCVVGK QNIYFNIQTE TRVSMTHFNL QDGACWFPLF RRRVPAPQSG VQKLDYAYKR
SYELSQLQKH IERKIMKKIS AWRTTRKTIW NRAFQPRLHK ILCDMEDLST FSRGRYDEPI
FSEQLEREFP NYRLYGFTLN FAYTNLAAIS ERIRTSCIHY NHDASVEFCV AVHLHAHAND
VLSVWLFLLS MVPLVE
