TRUD_PICTO
ID TRUD_PICTO Reviewed; 414 AA.
AC Q6L1R2;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Probable tRNA pseudouridine synthase D {ECO:0000255|HAMAP-Rule:MF_01082};
DE EC=5.4.99.27 {ECO:0000255|HAMAP-Rule:MF_01082};
DE AltName: Full=tRNA pseudouridine(13) synthase {ECO:0000255|HAMAP-Rule:MF_01082};
DE AltName: Full=tRNA pseudouridylate synthase D {ECO:0000255|HAMAP-Rule:MF_01082};
DE AltName: Full=tRNA-uridine isomerase D {ECO:0000255|HAMAP-Rule:MF_01082};
GN Name=truD {ECO:0000255|HAMAP-Rule:MF_01082}; OrderedLocusNames=PTO0505;
OS Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC
OS 100828).
OC Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC Picrophilaceae; Picrophilus.
OX NCBI_TaxID=263820;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828;
RX PubMed=15184674; DOI=10.1073/pnas.0401356101;
RA Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C.,
RA Schepers B., Dock C., Antranikian G., Liebl W.;
RT "Genome sequence of Picrophilus torridus and its implications for life
RT around pH 0.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004).
CC -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC uracil-13 in transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01082}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(13) in tRNA = pseudouridine(13) in tRNA;
CC Xref=Rhea:RHEA:42540, Rhea:RHEA-COMP:10105, Rhea:RHEA-COMP:10106,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.27;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01082};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family.
CC {ECO:0000255|HAMAP-Rule:MF_01082}.
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DR EMBL; AE017261; AAT43090.1; -; Genomic_DNA.
DR RefSeq; WP_011177306.1; NC_005877.1.
DR AlphaFoldDB; Q6L1R2; -.
DR SMR; Q6L1R2; -.
DR STRING; 263820.PTO0505; -.
DR EnsemblBacteria; AAT43090; AAT43090; PTO0505.
DR GeneID; 2844248; -.
DR KEGG; pto:PTO0505; -.
DR PATRIC; fig|263820.9.peg.532; -.
DR eggNOG; arCOG04252; Archaea.
DR HOGENOM; CLU_005281_4_1_2; -.
DR OMA; GFPNYFD; -.
DR OrthoDB; 92795at2157; -.
DR Proteomes; UP000000438; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.2350.20; -; 1.
DR HAMAP; MF_01082; TruD; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001656; PsdUridine_synth_TruD.
DR InterPro; IPR020119; PsdUridine_synth_TruD_CS.
DR InterPro; IPR011760; PsdUridine_synth_TruD_insert.
DR InterPro; IPR042214; TruD_catalytic.
DR PANTHER; PTHR13326; PTHR13326; 1.
DR Pfam; PF01142; TruD; 1.
DR PIRSF; PIRSF037016; Pseudouridin_synth_euk_prd; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00094; tRNA_TruD_broad; 1.
DR PROSITE; PS50984; TRUD; 1.
DR PROSITE; PS01268; UPF0024; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..414
FT /note="Probable tRNA pseudouridine synthase D"
FT /id="PRO_0000152548"
FT DOMAIN 162..382
FT /note="TRUD"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01082"
FT ACT_SITE 90
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01082"
SQ SEQUENCE 414 AA; 48171 MW; 9E5559745205456A CRC64;
MFYINIAYIF MDLGMYGYIT KTEKLNGMIK SNPEDFIVEE IPFDIKKDDN GKYLIIKARL
YDWDTNRFVM YLARHMNISR KRITYAGTKD KRAVTTQYFC INTDRMFSGS INGIEIIEQF
RSNDIIKLGD LYGNRFLIKV DYPGDLEKDS SETLKEINEK GGFPNFFGVQ RFGSMRSNTH
YIGKMIIKGE YEKAADKYLY DDNFDTEEYR INYGKNMDAK NSLKEYPGNL TFERSILGAI
ASGNKSSAFN ALPKNLSIMF VHAFQSYLFN KMLSERMKHV KDLKTVIEGD LLYNIDDYFN
PDKKRLIEAN RYNLEMLNNL SSMDKIRPVI PLPGFDTRMP DGLQRDIMLK VLEDENVSLQ
DFKIPGEYSY MSSSGDYRII SAKPINLKFP EKNKLDFILG RGIYATSFLR EIIK