TRUD_PSEAE
ID TRUD_PSEAE Reviewed; 355 AA.
AC Q9HY04;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=tRNA pseudouridine synthase D {ECO:0000255|HAMAP-Rule:MF_01082};
DE EC=5.4.99.27 {ECO:0000255|HAMAP-Rule:MF_01082};
DE AltName: Full=tRNA pseudouridine(13) synthase {ECO:0000255|HAMAP-Rule:MF_01082};
DE AltName: Full=tRNA pseudouridylate synthase D {ECO:0000255|HAMAP-Rule:MF_01082};
DE AltName: Full=tRNA-uridine isomerase D {ECO:0000255|HAMAP-Rule:MF_01082};
GN Name=truD {ECO:0000255|HAMAP-Rule:MF_01082}; OrderedLocusNames=PA3626;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-13 in
CC transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01082}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(13) in tRNA = pseudouridine(13) in tRNA;
CC Xref=Rhea:RHEA:42540, Rhea:RHEA-COMP:10105, Rhea:RHEA-COMP:10106,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.27;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01082};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family.
CC {ECO:0000255|HAMAP-Rule:MF_01082}.
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DR EMBL; AE004091; AAG07014.1; -; Genomic_DNA.
DR PIR; H83193; H83193.
DR RefSeq; NP_252316.1; NC_002516.2.
DR RefSeq; WP_003113870.1; NZ_QZGE01000001.1.
DR AlphaFoldDB; Q9HY04; -.
DR SMR; Q9HY04; -.
DR STRING; 287.DR97_4313; -.
DR PaxDb; Q9HY04; -.
DR PRIDE; Q9HY04; -.
DR DNASU; 880483; -.
DR EnsemblBacteria; AAG07014; AAG07014; PA3626.
DR GeneID; 880483; -.
DR KEGG; pae:PA3626; -.
DR PATRIC; fig|208964.12.peg.3795; -.
DR PseudoCAP; PA3626; -.
DR HOGENOM; CLU_005281_4_0_6; -.
DR InParanoid; Q9HY04; -.
DR OMA; LWLWVEK; -.
DR PhylomeDB; Q9HY04; -.
DR BioCyc; PAER208964:G1FZ6-3696-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0001522; P:pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.2340.10; -; 1.
DR Gene3D; 3.30.2350.20; -; 1.
DR HAMAP; MF_01082; TruD; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001656; PsdUridine_synth_TruD.
DR InterPro; IPR020119; PsdUridine_synth_TruD_CS.
DR InterPro; IPR011760; PsdUridine_synth_TruD_insert.
DR InterPro; IPR042214; TruD_catalytic.
DR InterPro; IPR043165; TruD_insert_sf.
DR Pfam; PF01142; TruD; 2.
DR SUPFAM; SSF55120; SSF55120; 1.
DR PROSITE; PS50984; TRUD; 1.
DR PROSITE; PS01268; UPF0024; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..355
FT /note="tRNA pseudouridine synthase D"
FT /id="PRO_0000152515"
FT DOMAIN 160..306
FT /note="TRUD"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01082"
FT ACT_SITE 84
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01082"
SQ SEQUENCE 355 AA; 38782 MW; 273DE5AF6063C565 CRC64;
MSVLGELDLL GPRAHGAACG EAVLKAVAED FQVDEVLEIP LSGEGEHLWL WVEKRGLNTE
EAARRLGRAA GVQQKNVSYA GLKDRQALTR QWFSLHLPGK ADPDLGAAEG ADLRILRCTR
HSRKLQRGAH AANGFTLRLT GLRAERAPLD ARLERIAADG VPNYFGLQRF GHGGGNLVDA
RSCAEQDLLP ANRNLRSRFL SAGRSYLFNR LLAERVAEGS WNRAAVGDLL AFTDSRSFFL
AGEEECRDAR LAALDLHPTG PLWGEGDPPS GAGVLDRELA LAGREPALCR WLAKAGMAHE
RRILRLPIQG LAWHYPEPDV LQLEFVLPAG CFATVVVREI LDLVPTGQTE NPCAY
