C2D61_ARATH
ID C2D61_ARATH Reviewed; 751 AA.
AC Q93XX4; Q9C8L5; Q9LPH4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=C2 domain-containing protein At1g53590;
DE AltName: Full=N-terminal-TM-C2 domain type 6 protein 1;
DE Short=NTMC2TYPE6.1;
GN Name=NTMC2T6.1; OrderedLocusNames=At1g53590; ORFNames=F22G10.28, T3F20.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION.
RX PubMed=17672888; DOI=10.1186/1471-2164-8-259;
RA Craxton M.;
RT "Evolutionary genomics of plant genes encoding N-terminal-TM-C2 domain
RT proteins and the similar FAM62 genes and synaptotagmin genes of
RT metazoans.";
RL BMC Genomics 8:259-259(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the extended synaptotagmin family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF78431.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG51985.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC018748; AAF78431.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC024260; AAG51985.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32967.1; -; Genomic_DNA.
DR EMBL; AY054685; AAK96876.1; -; mRNA.
DR PIR; A96576; A96576.
DR RefSeq; NP_564637.1; NM_104237.4.
DR AlphaFoldDB; Q93XX4; -.
DR SMR; Q93XX4; -.
DR STRING; 3702.AT1G53590.1; -.
DR iPTMnet; Q93XX4; -.
DR PaxDb; Q93XX4; -.
DR PRIDE; Q93XX4; -.
DR ProteomicsDB; 222824; -.
DR EnsemblPlants; AT1G53590.1; AT1G53590.1; AT1G53590.
DR GeneID; 841794; -.
DR Gramene; AT1G53590.1; AT1G53590.1; AT1G53590.
DR KEGG; ath:AT1G53590; -.
DR Araport; AT1G53590; -.
DR TAIR; locus:2024912; AT1G53590.
DR eggNOG; KOG1012; Eukaryota.
DR HOGENOM; CLU_017566_0_0_1; -.
DR InParanoid; Q93XX4; -.
DR OMA; WPFLGRM; -.
DR OrthoDB; 524702at2759; -.
DR PRO; PR:Q93XX4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q93XX4; baseline and differential.
DR Genevisible; Q93XX4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.150; -; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR031468; SMP_LBD.
DR Pfam; PF00168; C2; 1.
DR SMART; SM00239; C2; 1.
DR SUPFAM; SSF49562; SSF49562; 1.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS51847; SMP; 1.
PE 1: Evidence at protein level;
KW Calcium; Coiled coil; Lipid transport; Lipid-binding; Membrane;
KW Metal-binding; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..751
FT /note="C2 domain-containing protein At1g53590"
FT /id="PRO_0000315404"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 68..262
FT /note="SMP-LTD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01194"
FT DOMAIN 267..381
FT /note="C2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT REGION 469..519
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 572..751
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 701..728
FT /evidence="ECO:0000255"
FT COMPBIAS 472..486
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 487..511
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 582..638
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 666..685
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 692..751
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 298
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 298
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 304
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 352
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 352
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 354
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 354
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 359
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT CONFLICT 201
FT /note="F -> Y (in Ref. 3; AAK96876)"
FT /evidence="ECO:0000305"
FT CONFLICT 654
FT /note="C -> Y (in Ref. 3; AAK96876)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 751 AA; 84901 MW; 0AB8219A6B6A81D4 CRC64;
MESSLIHHII IVLLLLWFIS SLNRSHAFFY FLALIYLYLV HERYVMRLKR KLQFEERKQA
NQRRVLSDSE SVRWMNYAVE KIWPICMEQI ASQKILGPII PWFLEKYRPW TAKKAVIQHL
YMGRNPPLLT DIRVLRQSTG DDHLVLELGM NFLAADDMSA ILAVKLRKRL GFGMWTKLHL
TGMHVEGKVL IGVKFLRRWP FLGRLRVCFA EPPYFQMTVK PIFTHGLDVA VLPGIAGWLD
KLLSIAFEQT LVQPNMLVVD MEKFVSPTSE NWFFVDEKEP VAHVLVEVFE ASDLKPSDLN
GLADPYVKGK LGAYRFKTKI QKKTLSPKWH EEFKIPIFTW DSPSILNIEV GDKDRFVDDT
LGECSVNIEE FRGGQRNDMW LSLQNIKMGR LHLAITVIED NAKSSDDPLK KAKLNKEDIQ
TSFASDTTNL GSFSSDKSPS VVDNFEPIKI DGQEETAIWV QKPGAEVSQI WEPRKGKSRR
LDSQIQRTPN DESLSNGSSS TDDNQEGSKN PMKSVGRGLR KIGSMFHRNV KKEEFLIGSI
EEESQSQSPR INLKAVNQKD VGLNFIVDDN LSGPLSGKSL DGESLDAEEN SGKGHMKDVA
KSFLKQAEKS AKQIKHAFSR KGSMKPRDGH KEIVPESDSG TDSESSDDDD AFTCVKNLAT
EPGKLTRDGN IERTGDDDHV DSTTLATAKE DSSGDILEDS TDVEAKEEKL KEAAESETRD
MDTAMNIKTE DEKGDTLKNI EEGEEKESSS K
