TRUD_THET8
ID TRUD_THET8 Reviewed; 356 AA.
AC Q5SI49;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=tRNA pseudouridine synthase D {ECO:0000255|HAMAP-Rule:MF_01082};
DE EC=5.4.99.27 {ECO:0000255|HAMAP-Rule:MF_01082};
DE AltName: Full=tRNA pseudouridine(13) synthase {ECO:0000255|HAMAP-Rule:MF_01082};
DE AltName: Full=tRNA pseudouridylate synthase D {ECO:0000255|HAMAP-Rule:MF_01082};
DE AltName: Full=tRNA-uridine isomerase D {ECO:0000255|HAMAP-Rule:MF_01082};
GN Name=truD {ECO:0000255|HAMAP-Rule:MF_01082}; OrderedLocusNames=TTHA1531;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-13 in
CC transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01082}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(13) in tRNA = pseudouridine(13) in tRNA;
CC Xref=Rhea:RHEA:42540, Rhea:RHEA-COMP:10105, Rhea:RHEA-COMP:10106,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.27;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01082};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family.
CC {ECO:0000255|HAMAP-Rule:MF_01082}.
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DR EMBL; AP008226; BAD71354.1; -; Genomic_DNA.
DR RefSeq; WP_011228739.1; NC_006461.1.
DR RefSeq; YP_144797.1; NC_006461.1.
DR AlphaFoldDB; Q5SI49; -.
DR SMR; Q5SI49; -.
DR STRING; 300852.55772913; -.
DR PRIDE; Q5SI49; -.
DR EnsemblBacteria; BAD71354; BAD71354; BAD71354.
DR GeneID; 3169926; -.
DR KEGG; ttj:TTHA1531; -.
DR PATRIC; fig|300852.9.peg.1505; -.
DR eggNOG; COG0585; Bacteria.
DR HOGENOM; CLU_005281_4_0_0; -.
DR OMA; LWLWVEK; -.
DR PhylomeDB; Q5SI49; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.2340.10; -; 1.
DR Gene3D; 3.30.2350.20; -; 1.
DR HAMAP; MF_01082; TruD; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001656; PsdUridine_synth_TruD.
DR InterPro; IPR011760; PsdUridine_synth_TruD_insert.
DR InterPro; IPR042214; TruD_catalytic.
DR InterPro; IPR043165; TruD_insert_sf.
DR Pfam; PF01142; TruD; 2.
DR SUPFAM; SSF55120; SSF55120; 1.
DR PROSITE; PS50984; TRUD; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..356
FT /note="tRNA pseudouridine synthase D"
FT /id="PRO_0000152524"
FT DOMAIN 159..302
FT /note="TRUD"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01082"
FT ACT_SITE 84
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01082"
SQ SEQUENCE 356 AA; 40182 MW; 0BFB3FF6940A345C CRC64;
MDLVFRPERY PFLTQDLPGV GGEIRVEPED FQVEEVPAYL PKGEGEHLYL LLEKEGRTTR
EVLEFLRDEV GVPEKEIGVA GLKDKRAKTR QWFSIPRKYE DALCLLENLQ GVRLLAADLH
TNKLRTGHLK GNRFHILIRR PKGGVAEAEA VLKRLAEKGV PNYYGPQRFG LGGLNPVRGY
KLVKEGKGRG SPWLKRFLIG SLQSLLFNDW VALRMALGLY DRVVPGDWAK KHATGGEFLV
EDPGEAERAL RLEISATGPL FGKKYPEAQG EARAIEDEVL ARYGLKREEF RARRGARRPI
RVPLAEWKVE EAPEGLWLSF FLPKGSYATS LLREVMKVEA LDHLEAEPAP EDAEGL
