TRYB_DROME
ID TRYB_DROME Reviewed; 253 AA.
AC P35004; Q9V5Y3;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Trypsin beta;
DE EC=3.4.21.4;
DE Flags: Precursor;
GN Name=betaTry; ORFNames=CG18211;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10486967; DOI=10.1093/oxfordjournals.molbev.a026202;
RA Wang S., Magoulas C., Hickey D.A.;
RT "Concerted evolution within a trypsin gene cluster in Drosophila.";
RL Mol. Biol. Evol. 16:1117-1124(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Oregon-R;
RA Wang S., Magoulas C., Hickey D.A.;
RL Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00274}.
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DR EMBL; M96372; AAA28980.1; -; Genomic_DNA.
DR EMBL; U04853; AAA17451.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF58658.2; -; Genomic_DNA.
DR EMBL; BT016077; AAV36962.1; -; mRNA.
DR RefSeq; NP_001286314.1; NM_001299385.1.
DR RefSeq; NP_524904.1; NM_080165.3.
DR AlphaFoldDB; P35004; -.
DR SMR; P35004; -.
DR STRING; 7227.FBpp0087223; -.
DR MEROPS; S01.A83; -.
DR PaxDb; P35004; -.
DR PRIDE; P35004; -.
DR DNASU; 47901; -.
DR EnsemblMetazoa; FBtr0088122; FBpp0087223; FBgn0010357.
DR EnsemblMetazoa; FBtr0345928; FBpp0311842; FBgn0010357.
DR GeneID; 47901; -.
DR KEGG; dme:Dmel_CG18211; -.
DR UCSC; CG18211-RA; d. melanogaster.
DR CTD; 47901; -.
DR FlyBase; FBgn0010357; betaTry.
DR VEuPathDB; VectorBase:FBgn0010357; -.
DR eggNOG; KOG3627; Eukaryota.
DR GeneTree; ENSGT00940000164166; -.
DR HOGENOM; CLU_006842_7_1_1; -.
DR InParanoid; P35004; -.
DR OMA; YSARVIV; -.
DR OrthoDB; 1314811at2759; -.
DR PhylomeDB; P35004; -.
DR BioGRID-ORCS; 47901; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 47901; -.
DR PRO; PR:P35004; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0010357; Expressed in adult midgut (Drosophila) and 19 other tissues.
DR ExpressionAtlas; P35004; baseline and differential.
DR Genevisible; P35004; DM.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0017171; F:serine hydrolase activity; HDA:FlyBase.
DR GO; GO:0004252; F:serine-type endopeptidase activity; ISM:FlyBase.
DR GO; GO:0006508; P:proteolysis; ISM:FlyBase.
DR CDD; cd00190; Tryp_SPc; 1.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001314; Peptidase_S1A.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR InterPro; IPR033116; TRYPSIN_SER.
DR Pfam; PF00089; Trypsin; 1.
DR PRINTS; PR00722; CHYMOTRYPSIN.
DR SMART; SM00020; Tryp_SPc; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS50240; TRYPSIN_DOM; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
DR PROSITE; PS00135; TRYPSIN_SER; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Hydrolase; Protease; Reference proteome; Secreted;
KW Serine protease; Signal; Zymogen.
FT SIGNAL 1..22
FT /evidence="ECO:0000305"
FT PROPEP 23..30
FT /note="Activation peptide"
FT /id="PRO_0000028265"
FT CHAIN 31..253
FT /note="Trypsin beta"
FT /id="PRO_0000028266"
FT DOMAIN 31..253
FT /note="Peptidase S1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT ACT_SITE 71
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 116
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 210
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT SITE 204
FT /note="Required for specificity"
FT /evidence="ECO:0000250"
FT DISULFID 56..72
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 180..197
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 206..230
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT CONFLICT 53
FT /note="S -> R (in Ref. 1; AAA28980 and 2; AAA17451)"
FT /evidence="ECO:0000305"
FT CONFLICT 85
FT /note="A -> G (in Ref. 1; AAA28980 and 2; AAA17451)"
FT /evidence="ECO:0000305"
FT CONFLICT 114..115
FT /note="VN -> TS (in Ref. 1; AAA28980 and 2; AAA17451)"
FT /evidence="ECO:0000305"
FT CONFLICT 121
FT /note="H -> N (in Ref. 1; AAA28980 and 2; AAA17451)"
FT /evidence="ECO:0000305"
FT CONFLICT 142
FT /note="P -> T (in Ref. 1; AAA28980 and 2; AAA17451)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 253 AA; 25693 MW; 3F5D35D235B8D8CD CRC64;
MLKFLILLSA VACALGGTIP EGLLPQLDGR IVGGTATTIS SFPWQISLQR SGSHSCGGSI
YSARVIVTAA HCLQSVSASS LQIRAGSSYW SSGGVVAKVS SFKNHEGYNA NTMVNDIAVL
HLSSSLSFSS TIKAIGLASS NPANGAAASV SGWGTESSGS SSIPSQLRYV NVNIVSQSRC
SSSSYGYGNQ IKSSMICAFA SGKDSCQGDS GGPLVSGGVL VGVVSWGYGC AAANYPGVYA
DVAALRSWVI NNA
