TRYZ_DROME
ID TRYZ_DROME Reviewed; 280 AA.
AC P42280; Q9V5X8;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Trypsin zeta;
DE EC=3.4.21.4;
DE Flags: Precursor;
GN Name=zetaTry; ORFNames=CG12387;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10486967; DOI=10.1093/oxfordjournals.molbev.a026202;
RA Wang S., Magoulas C., Hickey D.A.;
RT "Concerted evolution within a trypsin gene cluster in Drosophila.";
RL Mol. Biol. Evol. 16:1117-1124(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00274}.
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DR EMBL; U04853; AAA17456.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF58663.1; -; Genomic_DNA.
DR EMBL; AY113523; AAM29528.1; -; mRNA.
DR RefSeq; NP_523691.1; NM_078967.4.
DR AlphaFoldDB; P42280; -.
DR SMR; P42280; -.
DR BioGRID; 62009; 2.
DR IntAct; P42280; 4.
DR STRING; 7227.FBpp0087260; -.
DR MEROPS; S01.116; -.
DR PaxDb; P42280; -.
DR DNASU; 36216; -.
DR EnsemblMetazoa; FBtr0088164; FBpp0087260; FBgn0011556.
DR GeneID; 36216; -.
DR KEGG; dme:Dmel_CG12387; -.
DR UCSC; CG12387-RA; d. melanogaster.
DR CTD; 36216; -.
DR FlyBase; FBgn0011556; zetaTry.
DR VEuPathDB; VectorBase:FBgn0011556; -.
DR eggNOG; KOG3627; Eukaryota.
DR GeneTree; ENSGT00940000164166; -.
DR HOGENOM; CLU_006842_7_1_1; -.
DR InParanoid; P42280; -.
DR OMA; CALPTHP; -.
DR OrthoDB; 1314811at2759; -.
DR PhylomeDB; P42280; -.
DR BioGRID-ORCS; 36216; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36216; -.
DR PRO; PR:P42280; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0011556; Expressed in midgut and 13 other tissues.
DR Genevisible; P42280; DM.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0017171; F:serine hydrolase activity; HDA:FlyBase.
DR GO; GO:0004252; F:serine-type endopeptidase activity; ISM:FlyBase.
DR GO; GO:0006508; P:proteolysis; ISM:FlyBase.
DR CDD; cd00190; Tryp_SPc; 1.
DR Gene3D; 2.40.10.10; -; 1.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001314; Peptidase_S1A.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR InterPro; IPR033116; TRYPSIN_SER.
DR Pfam; PF00089; Trypsin; 1.
DR PRINTS; PR00722; CHYMOTRYPSIN.
DR SMART; SM00020; Tryp_SPc; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS50240; TRYPSIN_DOM; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
DR PROSITE; PS00135; TRYPSIN_SER; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Hydrolase; Protease; Reference proteome; Secreted;
KW Serine protease; Signal; Zymogen.
FT SIGNAL 1..22
FT /evidence="ECO:0000305"
FT PROPEP 23..38
FT /note="Activation peptide"
FT /id="PRO_0000028287"
FT CHAIN 39..280
FT /note="Trypsin zeta"
FT /id="PRO_0000028288"
FT DOMAIN 39..278
FT /note="Peptidase S1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT ACT_SITE 87
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 134
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 234
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT SITE 228
FT /note="Required for specificity"
FT /evidence="ECO:0000250"
FT DISULFID 72..88
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 198..218
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 230..254
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT CONFLICT 86
FT /note="A -> G (in Ref. 1; AAA17456)"
FT /evidence="ECO:0000305"
FT CONFLICT 145
FT /note="P -> A (in Ref. 1; AAA17456)"
FT /evidence="ECO:0000305"
FT CONFLICT 153
FT /note="A -> G (in Ref. 1; AAA17456)"
FT /evidence="ECO:0000305"
FT CONFLICT 158
FT /note="L -> S (in Ref. 1; AAA17456)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="R -> P (in Ref. 1; AAA17456)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 280 AA; 29738 MW; 99A2D76557C308FC CRC64;
MSSSWIVGLL AFLVSLVALT QGLPLLEDLD EKSVPDGRIV GGYATDIAQV PYQISLRYKG
ITTPENPFRH RCGGSIFNET TIVTAAHCVI GTVASQYKVV AGTNFQTGSD GVITNVKEIV
MHEGYYSGAA YNNDIAILFV DPPLPLNNFT IKAIKLALEQ PIEGTVSKVS GWGTTSPGGY
SSNQLLAVDV PIVSNELCDQ DYEDFGDETY RITSAMLCAG KRGVGGADAC QGDSGGPLAV
RDELYGVVSW GNSCALPNYP GVYANVAYLR PWIDAVLAGL
