TS101_DICDI
ID TS101_DICDI Reviewed; 478 AA.
AC Q54LJ3;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=ESCRT-I complex subunit tsg101;
GN Name=tsg101; ORFNames=DDB_G0286797;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular
CC trafficking process. Required for the sorting of endocytic
CC ubiquitinated cargos into multivesicular bodies. May be involved in
CC cell growth and differentiation. Acts as a negative growth regulator
CC (By similarity). {ECO:0000250|UniProtKB:Q99816}.
CC -!- SUBUNIT: Component of the ESCRT-I complex (endosomal sorting complex
CC required for transport I). {ECO:0000250|UniProtKB:Q99816}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}. Nucleus {ECO:0000250}. Late
CC endosome membrane {ECO:0000250}; Peripheral membrane protein
CC {ECO:0000250}.
CC -!- DOMAIN: The UEV domain is required for the interaction of the complex
CC with ubiquitin. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UEV
CC subfamily. {ECO:0000305}.
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DR EMBL; AAFI02000089; EAL64154.1; -; Genomic_DNA.
DR RefSeq; XP_637568.1; XM_632476.1.
DR AlphaFoldDB; Q54LJ3; -.
DR SMR; Q54LJ3; -.
DR STRING; 44689.DDB0234022; -.
DR PaxDb; Q54LJ3; -.
DR PRIDE; Q54LJ3; -.
DR ABCD; Q54LJ3; 3 sequenced antibodies.
DR EnsemblProtists; EAL64154; EAL64154; DDB_G0286797.
DR GeneID; 8625708; -.
DR KEGG; ddi:DDB_G0286797; -.
DR dictyBase; DDB_G0286797; tsg101.
DR eggNOG; KOG2391; Eukaryota.
DR HOGENOM; CLU_017548_2_0_1; -.
DR InParanoid; Q54LJ3; -.
DR OMA; LWLPEPY; -.
DR PhylomeDB; Q54LJ3; -.
DR Reactome; R-DDI-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR PRO; PR:Q54LJ3; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0000813; C:ESCRT I complex; ISS:dictyBase.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0140220; C:pathogen-containing vacuole; IDA:dictyBase.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0008333; P:endosome to lysosome transport; IBA:GO_Central.
DR GO; GO:0001778; P:plasma membrane repair; IMP:dictyBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0009611; P:response to wounding; IDA:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR037202; ESCRT_assembly_dom.
DR InterPro; IPR017916; SB_dom.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR InterPro; IPR008883; UEV_N.
DR Pfam; PF05743; UEV; 1.
DR Pfam; PF09454; Vps23_core; 1.
DR SUPFAM; SSF140111; SSF140111; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS51312; SB; 1.
DR PROSITE; PS51322; UEV; 1.
PE 3: Inferred from homology;
KW Coiled coil; Cytoplasm; Endosome; Growth regulation; Membrane; Nucleus;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..478
FT /note="ESCRT-I complex subunit tsg101"
FT /id="PRO_0000365724"
FT DOMAIN 24..172
FT /note="UEV"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00652"
FT DOMAIN 386..454
FT /note="SB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00644"
FT REGION 144..290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 458..478
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 321..372
FT /evidence="ECO:0000255"
FT COMPBIAS 144..158
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 167..184
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..222
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 223..237
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 261..275
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 478 AA; 54919 MW; 294FE5E2DDF54A8C CRC64;
MYGHHGYPMH AHQQQMVNPT LAIVDNKMHT LSTFLNYIRA YRDPLRISKD LKETFHLFPN
LSPFYENIPN RVNLICIKGT IPICFKGINY YLPIIVWVPL NYPQEFPTMV LDPTPEMRIV
KNHHHVNLQG LVYHPYISSW SSNSTMETRV SQQQQPQPPQ NNISPPPYGS SPTNNNVAAQ
QQQPPPPYGS SPSTSNSSSY TQPPPSYDSI KNKTNNTSSL PPPKQPQTSP PPPPTQQQQQ
QQNNNIIPPP QQPSPPPAYT EPTAAKQQQQ QTSPSVKPLP PLPPQPVIDK KSEMVDQCTI
KLQELLSKFY DTTSLEIKDF EAHNKSLEEL SKKKLLEKEE LSNQLATYNS QIDQLNENIE
QLEKWINEND KSDSNIDIDQ ILGPKDSLSK QLLKLVSDDS TIEDLLYYLD KALHSNRISL
EEYLKNVRSL SRDQFIIRAT VKKVQFIIRQ NQQQLQQQQQ QQNSPQRQQY NQQQYFSK
