ID   TS15A_SOLHA             Reviewed;         498 AA.
AC   P0DO48;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   23-FEB-2022, sequence version 1.
DT   03-AUG-2022, entry version 3.
DE   RecName: Full=Sesquiterpene synthase 15a {ECO:0000303|PubMed:21818683};
DE            Short=ShTPS15a {ECO:0000303|PubMed:21818683};
GN   Name=TPS15a {ECO:0000303|PubMed:21818683};
OS   Solanum habrochaites (Wild tomato) (Lycopersicon hirsutum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=62890;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PATHWAY, AND GENE FAMILY.
RC   STRAIN=cv. PI127826;
RX   PubMed=21818683; DOI=10.1007/s11103-011-9813-x;
RA   Bleeker P.M., Spyropoulou E.A., Diergaarde P.J., Volpin H., De Both M.T.J.,
RA   Zerbe P., Bohlmann J., Falara V., Matsuba Y., Pichersky E., Haring M.A.,
RA   Schuurink R.C.;
RT   "RNA-seq discovery, functional characterization, and comparison of
RT   sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites
RT   trichomes.";
RL   Plant Mol. Biol. 77:323-336(2011).
CC   -!- FUNCTION: Sesquiterpene synthase involved in the biosynthesis of
CC       volatile compounds (PubMed:21818683). No activity detected with geranyl
CC       diphosphate (GPP) and farnesyl diphosphate (FPP) as substrates
CC       (PubMed:21818683). {ECO:0000269|PubMed:21818683}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:A0A1C9J6A7};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000250|UniProtKB:A0A1C9J6A7};
CC       Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit.
CC       {ECO:0000250|UniProtKB:A0A1C9J6A7};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000269|PubMed:21818683}.
CC   -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC       the catalytic activity, presumably through binding to Mg(2+).
CC       {ECO:0000250|UniProtKB:Q40577}.
CC   -!- SIMILARITY: Belongs to the terpene synthase family. Tpsa subfamily.
CC       {ECO:0000305}.
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DR   EMBL; JN402397; -; NOT_ANNOTATED_CDS; mRNA.
DR   UniPathway; UPA00213; -.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR   GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   Gene3D; 1.50.10.130; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR   InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR   InterPro; IPR001906; Terpene_synth_N.
DR   InterPro; IPR036965; Terpene_synth_N_sf.
DR   InterPro; IPR005630; Terpene_synthase_metal-bd.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF01397; Terpene_synth; 1.
DR   Pfam; PF03936; Terpene_synth_C; 1.
DR   SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   2: Evidence at transcript level;
KW   Magnesium; Metal-binding.
FT   CHAIN           1..498
FT                   /note="Sesquiterpene synthase 15a"
FT                   /id="PRO_0000454686"
FT   MOTIF           250..254
FT                   /note="DDXXD motif"
FT                   /evidence="ECO:0000250|UniProtKB:A0A1C9J6A7"
FT   BINDING         250
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         250
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         254
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         254
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
FT   BINDING         403
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250|UniProtKB:Q40577"
SQ   SEQUENCE   498 AA;  58141 MW;  B00FD459C04C07E2 CRC64;
     MLVLSPSKST QKLEFINTIQ LLGVSYHFEH EIEESLSEIY NGYEEWIGES HNLHAVALSF
     RLLRQQGYYV SSDVFRKFTD DQGNYNKELV NDTHGLLSLY EAAHFRVHDE EILDEAINFT
     TTHFKLLLPK LSNSLSMQVS YALKYPINKT IARAATRKYI SFYQEEKSSC DQVLINFAKL
     DFNILQKMYK REMCDITRWW KELDLANELG FARDRVVELY FWSLGVYFEP QYKVARNILT
     KVLCFVSITD DIYDTYGTLH ELTLLTNAIE RRNIDAIENL TSYMKLFFTA LLHFYDEVEK
     ELEKENKSFR VNFAISEMKK LVRAYFQEAK WYHGNTVPKM EEEYMKNGIQ SSANPTLATA
     SWLGMGDEAT KEAFEWISTE PPILVASSNI ARLLNDIVSH EREIERGDVA SSIECYMNEY
     GATKEEAYME IRKIIENNWK DLNRGCLKPT TVPRVLLMPV LNLTRVAEFV YKDEDAYTFS
     KNNLKDVIFM VLDDPIEE