TS1R1_MOUSE
ID TS1R1_MOUSE Reviewed; 842 AA.
AC Q99PG6; Q6NS58; Q923J9; Q925I5; Q99PG5;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 133.
DE RecName: Full=Taste receptor type 1 member 1;
DE AltName: Full=G-protein coupled receptor 70;
DE Flags: Precursor;
GN Name=Tas1r1; Synonyms=Gpr70, T1r1, Tr1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11509186; DOI=10.1016/s0092-8674(01)00451-2;
RA Nelson G., Hoon M.A., Chandrashekar J., Zhang Y., Ryba N.J.P., Zuker C.S.;
RT "Mammalian sweet taste receptors.";
RL Cell 106:381-390(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS ASP-244; ALA-281;
RP 309-TYR--PRO-314; THR-347; TYR-418; ASN-443 AND GLU-626.
RC STRAIN=129/J, and C57BL/6ByJ;
RX PubMed=11178737; DOI=10.1007/s003350010236;
RA Li X., Inoue M., Reed D.R., Huque T., Puchalski R.B., Tordoff M.G.,
RA Ninomiya Y., Beauchamp G.K., Bachmanov A.A.;
RT "High-resolution genetic mapping of the saccharin preference locus (Sac)
RT and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal
RT chromosome 4.";
RL Mamm. Genome 12:13-16(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Circumvallate papilla, and Foliate papilla;
RX PubMed=11319557; DOI=10.1038/87440;
RA Montmayeur J.-P., Liberles S.D., Matsunami H., Buck L.B.;
RT "A candidate taste receptor gene near a sweet taste locus.";
RL Nat. Neurosci. 4:492-498(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=12892531; DOI=10.1290/1543-706x(2003)039<0080:trtiae>2.0.co;2;
RA Ariyasu T., Matsumoto S., Kyono F., Hanaya T., Arai S., Ikeda M.,
RA Kurimoto M.;
RT "Taste receptor T1R3 is an essential molecule for the cellular recognition
RT of the disaccharide trehalose.";
RL In Vitro Cell. Dev. Biol. Anim. 39:80-88(2003).
RN [5]
RP FUNCTION.
RX PubMed=11894099; DOI=10.1038/nature726;
RA Nelson G., Chandrashekar J., Hoon M.A., Feng L., Zhao G., Ryba N.J.P.,
RA Zuker C.S.;
RT "An amino-acid taste receptor.";
RL Nature 416:199-202(2002).
CC -!- FUNCTION: Putative taste receptor. TAS1R1/TAS1R3 responds to the umami
CC taste stimulus (the taste of monosodium glutamate) and also to most of
CC the 20 standard L-amino acids, but not to their D-enantiomers or other
CC compounds. Sequence differences within and between species can
CC significantly influence the selectivity and specificity of taste
CC responses. {ECO:0000269|PubMed:11894099}.
CC -!- SUBUNIT: Forms heterodimers with TAS1R3.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed strongly only in fungiform papillae.
CC {ECO:0000269|PubMed:11319557}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 3 family. TAS1R
CC subfamily. {ECO:0000305}.
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DR EMBL; AY032622; AAK51603.1; -; mRNA.
DR EMBL; AF301161; AAK07091.1; -; mRNA.
DR EMBL; AF301162; AAK07092.1; -; mRNA.
DR EMBL; AF337040; AAK39437.1; -; mRNA.
DR CCDS; CCDS18985.1; -.
DR RefSeq; NP_114073.1; NM_031867.2.
DR AlphaFoldDB; Q99PG6; -.
DR SMR; Q99PG6; -.
DR STRING; 10090.ENSMUSP00000030792; -.
DR GlyGen; Q99PG6; 7 sites.
DR PhosphoSitePlus; Q99PG6; -.
DR EPD; Q99PG6; -.
DR PaxDb; Q99PG6; -.
DR PRIDE; Q99PG6; -.
DR DNASU; 110326; -.
DR GeneID; 110326; -.
DR KEGG; mmu:110326; -.
DR CTD; 80835; -.
DR MGI; MGI:1927505; Tas1r1.
DR eggNOG; KOG1056; Eukaryota.
DR InParanoid; Q99PG6; -.
DR OrthoDB; 136715at2759; -.
DR PhylomeDB; Q99PG6; -.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR Reactome; R-MMU-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-MMU-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR BioGRID-ORCS; 110326; 5 hits in 71 CRISPR screens.
DR PRO; PR:Q99PG6; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q99PG6; protein.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0008527; F:taste receptor activity; ISO:MGI.
DR GO; GO:0050917; P:sensory perception of umami taste; ISO:MGI.
DR Gene3D; 2.10.50.30; -; 1.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR000337; GPCR_3.
DR InterPro; IPR011500; GPCR_3_9-Cys_dom.
DR InterPro; IPR038550; GPCR_3_9-Cys_sf.
DR InterPro; IPR017978; GPCR_3_C.
DR InterPro; IPR000068; GPCR_3_Ca_sens_rcpt-rel.
DR InterPro; IPR017979; GPCR_3_CS.
DR InterPro; IPR028082; Peripla_BP_I.
DR PANTHER; PTHR24061; PTHR24061; 1.
DR Pfam; PF00003; 7tm_3; 1.
DR Pfam; PF01094; ANF_receptor; 1.
DR Pfam; PF07562; NCD3G; 1.
DR PRINTS; PR00592; CASENSINGR.
DR PRINTS; PR00248; GPCRMGR.
DR SUPFAM; SSF53822; SSF53822; 1.
DR PROSITE; PS00980; G_PROTEIN_RECEP_F3_2; 1.
DR PROSITE; PS00981; G_PROTEIN_RECEP_F3_3; 1.
DR PROSITE; PS50259; G_PROTEIN_RECEP_F3_4; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Sensory transduction; Signal; Taste;
KW Transducer; Transmembrane; Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..842
FT /note="Taste receptor type 1 member 1"
FT /id="PRO_0000012955"
FT TOPO_DOM 20..568
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 569..589
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 590..604
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 605..625
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 626..640
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 641..661
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 662..681
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 682..702
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 703..725
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 726..746
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 747..762
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 763..783
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 784..789
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 790..810
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 811..842
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 96
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 136
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 292
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 480
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 530
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 244
FT /note="N -> D (in strain: 129/J)"
FT /evidence="ECO:0000269|PubMed:11178737"
FT VARIANT 281
FT /note="D -> A (in strain: 129/J)"
FT /evidence="ECO:0000269|PubMed:11178737"
FT VARIANT 309..314
FT /note="NIPNVS -> YITNVP (in strain: 129/J)"
FT VARIANT 347
FT /note="M -> T (in strain: 129/J)"
FT /evidence="ECO:0000269|PubMed:11178737"
FT VARIANT 418
FT /note="H -> Y (in strain: 129/J)"
FT /evidence="ECO:0000269|PubMed:11178737"
FT VARIANT 443
FT /note="K -> N (in strain: 129/J)"
FT /evidence="ECO:0000269|PubMed:11178737"
FT VARIANT 626
FT /note="K -> E (in strain: 129/J)"
FT /evidence="ECO:0000269|PubMed:11178737"
FT CONFLICT 120
FT /note="L -> P (in Ref. 3; AAK51603)"
FT /evidence="ECO:0000305"
FT CONFLICT 648
FT /note="S -> P (in Ref. 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 649
FT /note="C -> F (in Ref. 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 655
FT /note="F -> S (in Ref. 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 659
FT /note="I -> V (in Ref. 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 776
FT /note="A -> V (in Ref. 4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 842 AA; 93426 MW; 58826C43F5DD352E CRC64;
MLFWAAHLLL SLQLAVAYCW AFSCQRTESS PGFSLPGDFL LAGLFSLHAD CLQVRHRPLV
TSCDRSDSFN GHGYHLFQAM RFTVEEINNS TALLPNITLG YELYDVCSES SNVYATLRVL
AQQGTGHLEM QRDLRNHSSK VVALIGPDNT DHAVTTAALL SPFLMPLVSY EASSVILSGK
RKFPSFLRTI PSDKYQVEVI VRLLQSFGWV WISLVGSYGD YGQLGVQALE ELATPRGICV
AFKNVVPLSA QAGDPRMQRM MLRLARARTT VVVVFSNRHL DGVFFRSVVL ANLTGKVWIA
SEDWAISTNI PNVSGIQGIG TVLGVAIQQR QVPGLKEFEE SYVQAVMGAP RTCPEGSWCG
TNQLCRECHA FTTWNMPELG AFSMSAAYNV YEAVYAVAHG LHQLLGCTSG TCARGPVHPW
QLLQQIYKVN FLLHKKTVAF DDKGDPLGYY DIIAWDWNGP EWTFEVIGSA SLSPVHLDIN
KTKIQWHGKN NQVPVSVCTR DCLEGHHRLV MGSHHCCFEC MPCEAGTFLN TSELHTCQPC
GTEEWAPEGS SACFSRTVEF LGWHEPISLV LLAANTLLLL LLIGTAGLFA WRLHTPVVRS
AGGRLCFLML GSLVAGSCSL YSFFGKPTVP ACLLRQPLFS LGFAIFLSCL TIRSFQLVII
FKFSTKVPTF YHTWAQNHGA GIFVIVSSTV HLFLCLTWLA MWTPRPTREY QRFPHLVILE
CTEVNSVGFL VAFAHNILLS ISTFVCSYLG KELPENYNEA KCVTFSLLLH FVSWIAFFTM
SSIYQGSYLP AVNVLAGLAT LSGGFSGYFL PKCYVILCRP ELNNTEHFQA SIQDYTRRCG
TT
