TS1R2_MOUSE
ID TS1R2_MOUSE Reviewed; 843 AA.
AC Q925I4; B1AXX0; Q923J8;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Taste receptor type 1 member 2;
DE AltName: Full=G-protein coupled receptor 71;
DE AltName: Full=Sweet taste receptor T1R2;
DE Flags: Precursor;
GN Name=Tas1r2; Synonyms=Gpr71, T1r2, Tr2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND VARIANTS GLY-350 AND
RP ARG-352.
RC STRAIN=C57BL/6J, and DBA/2J;
RX PubMed=11319557; DOI=10.1038/87440;
RA Montmayeur J.-P., Liberles S.D., Matsunami H., Buck L.B.;
RT "A candidate taste receptor gene near a sweet taste locus.";
RL Nat. Neurosci. 4:492-498(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11509186; DOI=10.1016/s0092-8674(01)00451-2;
RA Nelson G., Hoon M.A., Chandrashekar J., Zhang Y., Ryba N.J.P., Zuker C.S.;
RT "Mammalian sweet taste receptors.";
RL Cell 106:381-390(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=12892531; DOI=10.1290/1543-706x(2003)039<0080:trtiae>2.0.co;2;
RA Ariyasu T., Matsumoto S., Kyono F., Hanaya T., Arai S., Ikeda M.,
RA Kurimoto M.;
RT "Taste receptor T1R3 is an essential molecule for the cellular recognition
RT of the disaccharide trehalose.";
RL In Vitro Cell. Dev. Biol. Anim. 39:80-88(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Putative taste receptor. TAS1R2/TAS1R3 recognizes diverse
CC natural and synthetic sweeteners.
CC -!- SUBUNIT: Forms heterodimers with TAS1R3.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed mainly in circumvallate and foliate taste
CC papillae. {ECO:0000269|PubMed:11319557}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 3 family. TAS1R
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF337041; AAK39438.1; -; mRNA.
DR EMBL; AY032623; AAK51604.1; -; mRNA.
DR EMBL; AL831790; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS18849.1; -.
DR RefSeq; NP_114079.1; NM_031873.1.
DR AlphaFoldDB; Q925I4; -.
DR SMR; Q925I4; -.
DR IntAct; Q925I4; 1.
DR STRING; 10090.ENSMUSP00000030510; -.
DR GlyGen; Q925I4; 9 sites.
DR PhosphoSitePlus; Q925I4; -.
DR PaxDb; Q925I4; -.
DR PRIDE; Q925I4; -.
DR Antibodypedia; 14734; 207 antibodies from 31 providers.
DR DNASU; 83770; -.
DR Ensembl; ENSMUST00000030510; ENSMUSP00000030510; ENSMUSG00000028738.
DR GeneID; 83770; -.
DR KEGG; mmu:83770; -.
DR UCSC; uc008vmr.1; mouse.
DR CTD; 80834; -.
DR MGI; MGI:1933546; Tas1r2.
DR VEuPathDB; HostDB:ENSMUSG00000028738; -.
DR eggNOG; KOG1056; Eukaryota.
DR GeneTree; ENSGT00940000156136; -.
DR HOGENOM; CLU_005389_5_1_1; -.
DR InParanoid; Q925I4; -.
DR OMA; TICISCI; -.
DR OrthoDB; 136715at2759; -.
DR PhylomeDB; Q925I4; -.
DR TreeFam; TF331269; -.
DR Reactome; R-MMU-418594; G alpha (i) signalling events.
DR Reactome; R-MMU-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-MMU-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR BioGRID-ORCS; 83770; 5 hits in 73 CRISPR screens.
DR PRO; PR:Q925I4; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q925I4; protein.
DR ExpressionAtlas; Q925I4; differential.
DR Genevisible; Q925I4; MM.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043235; C:receptor complex; ISO:MGI.
DR GO; GO:1903767; C:sweet taste receptor complex; ISO:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0033041; F:sweet taste receptor activity; IDA:CACAO.
DR GO; GO:0008527; F:taste receptor activity; ISO:MGI.
DR GO; GO:0001582; P:detection of chemical stimulus involved in sensory perception of sweet taste; ISO:MGI.
DR GO; GO:0032467; P:positive regulation of cytokinesis; ISO:MGI.
DR GO; GO:0050916; P:sensory perception of sweet taste; IGI:MGI.
DR Gene3D; 2.10.50.30; -; 1.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR000337; GPCR_3.
DR InterPro; IPR011500; GPCR_3_9-Cys_dom.
DR InterPro; IPR038550; GPCR_3_9-Cys_sf.
DR InterPro; IPR017978; GPCR_3_C.
DR InterPro; IPR000068; GPCR_3_Ca_sens_rcpt-rel.
DR InterPro; IPR017979; GPCR_3_CS.
DR InterPro; IPR028082; Peripla_BP_I.
DR PANTHER; PTHR24061; PTHR24061; 1.
DR Pfam; PF00003; 7tm_3; 1.
DR Pfam; PF01094; ANF_receptor; 1.
DR Pfam; PF07562; NCD3G; 1.
DR PRINTS; PR00248; GPCRMGR.
DR SUPFAM; SSF53822; SSF53822; 1.
DR PROSITE; PS00980; G_PROTEIN_RECEP_F3_2; 1.
DR PROSITE; PS50259; G_PROTEIN_RECEP_F3_4; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Sensory transduction; Signal; Taste;
KW Transducer; Transmembrane; Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..843
FT /note="Taste receptor type 1 member 2"
FT /id="PRO_0000012958"
FT TOPO_DOM 20..570
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 571..591
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 592..606
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 607..627
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 628..642
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 643..663
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 664..682
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 683..703
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 704..731
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 732..752
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 753..764
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 765..785
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 786..789
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 790..810
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 811..843
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 87
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 296
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 316
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 355
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 372
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 432
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 484
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 491
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 531
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 350
FT /note="E -> G (in strain: DBA/2J)"
FT /evidence="ECO:0000269|PubMed:11319557"
FT VARIANT 352
FT /note="P -> R (in strain: DBA/2J)"
FT /evidence="ECO:0000269|PubMed:11319557"
FT CONFLICT 175
FT /note="R -> Q (in Ref. 2; AAK51604)"
FT /evidence="ECO:0000305"
FT CONFLICT 527
FT /note="G -> D (in Ref. 2; AAK51604)"
FT /evidence="ECO:0000305"
FT CONFLICT 695
FT /note="A -> V (in Ref. 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 701
FT /note="M -> T (in Ref. 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 843 AA; 95736 MW; 0543834EA4E7AC2E CRC64;
MGPQARTLHL LFLLLHALPK PVMLVGNSDF HLAGDYLLGG LFTLHANVKS VSHLSYLQVP
KCNEYNMKVL GYNLMQAMRF AVEEINNCSS LLPGVLLGYE MVDVCYLSNN IQPGLYFLSQ
IDDFLPILKD YSQYRPQVVA VIGPDNSESA ITVSNILSYF LVPQVTYSAI TDKLRDKRRF
PAMLRTVPSA THHIEAMVQL MVHFQWNWIV VLVSDDDYGR ENSHLLSQRL TNTGDICIAF
QEVLPVPEPN QAVRPEEQDQ LDNILDKLRR TSARVVVIFS PELSLHNFFR EVLRWNFTGF
VWIASESWAI DPVLHNLTEL RHTGTFLGVT IQRVSIPGFS QFRVRHDKPE YPMPNETSLR
TTCNQDCDAC MNITESFNNV LMLSGERVVY SVYSAVYAVA HTLHRLLHCN QVRCTKQIVY
PWQLLREIWH VNFTLLGNQL FFDEQGDMPM LLDIIQWQWG LSQNPFQSIA SYSPTETRLT
YISNVSWYTP NNTVPISMCS KSCQPGQMKK PIGLHPCCFE CVDCPPGTYL NRSVDEFNCL
SCPGSMWSYK NNIACFKRRL AFLEWHEVPT IVVTILAALG FISTLAILLI FWRHFQTPMV
RSAGGPMCFL MLVPLLLAFG MVPVYVGPPT VFSCFCRQAF FTVCFSVCLS CITVRSFQIV
CVFKMARRLP SAYGFWMRYH GPYVFVAFIT AVKVALVAGN MLATTINPIG RTDPDDPNII
ILSCHPNYRN GLLFNTSMDL LLSVLGFSFA YVGKELPTNY NEAKFITLSM TFSFTSSISL
CTFMSVHDGV LVTIMDLLVT VLNFLAIGLG YFGPKCYMIL FYPERNTSAY FNSMIQGYTM
RKS
