TS1R3_HUMAN
ID TS1R3_HUMAN Reviewed; 852 AA.
AC Q7RTX0; Q5TA49; Q8NGW9;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 2.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Taste receptor type 1 member 3;
DE AltName: Full=Sweet taste receptor T1R3;
DE Flags: Precursor;
GN Name=TAS1R3; Synonyms=T1R3, TR3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S.,
RA Tsutsumi S., Aburatani H., Asai K., Akiyama Y.;
RT "Genome-wide discovery and analysis of human seven transmembrane helix
RT receptor genes.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=11917125; DOI=10.1073/pnas.072090199;
RA Li X., Staszewski L., Xu H., Durick K., Zoller M., Adler E.;
RT "Human receptors for sweet and umami taste.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:4692-4696(2002).
RN [4]
RP FUNCTION.
RX PubMed=12892531; DOI=10.1290/1543-706x(2003)039<0080:trtiae>2.0.co;2;
RA Ariyasu T., Matsumoto S., Kyono F., Hanaya T., Arai S., Ikeda M.,
RA Kurimoto M.;
RT "Taste receptor T1R3 is an essential molecule for the cellular recognition
RT of the disaccharide trehalose.";
RL In Vitro Cell. Dev. Biol. Anim. 39:80-88(2003).
RN [5]
RP REGION IMPLICATED IN SWEET RECEPTOR FUNCTION, AND MUTAGENESIS OF ALA-537
RP AND PHE-540.
RX PubMed=15299024; DOI=10.1074/jbc.m406779200;
RA Jiang P., Ji Q., Liu Z., Snyder L.A., Benard L.M.J., Margolskee R.F.,
RA Max M.;
RT "The cysteine-rich region of T1R3 determines responses to intensely sweet
RT proteins.";
RL J. Biol. Chem. 279:45068-45075(2004).
CC -!- FUNCTION: Putative taste receptor. TAS1R1/TAS1R3 responds to the umami
CC taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3
CC recognizes diverse natural and synthetic sweeteners. TAS1R3 is
CC essential for the recognition and response to the disaccharide
CC trehalose (By similarity). Sequence differences within and between
CC species can significantly influence the selectivity and specificity of
CC taste responses. {ECO:0000250, ECO:0000269|PubMed:11917125,
CC ECO:0000269|PubMed:12892531}.
CC -!- SUBUNIT: Forms homodimers or heterodimers with TAS1R1 and TAS1R2.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 3 family. TAS1R
CC subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=The taste experience - Issue
CC 55 of February 2005;
CC URL="https://web.expasy.org/spotlight/back_issues/055";
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DR EMBL; AB065647; BAC05873.1; -; Genomic_DNA.
DR EMBL; AL139287; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK000152; DAA00013.1; -; mRNA.
DR CCDS; CCDS30556.1; -.
DR RefSeq; NP_689414.1; NM_152228.2.
DR AlphaFoldDB; Q7RTX0; -.
DR SMR; Q7RTX0; -.
DR BioGRID; 123757; 94.
DR STRING; 9606.ENSP00000344411; -.
DR BindingDB; Q7RTX0; -.
DR ChEMBL; CHEMBL1613742; -.
DR DrugBank; DB00168; Aspartame.
DR TCDB; 9.A.14.7.10; the g-protein-coupled receptor (gpcr) family.
DR GlyGen; Q7RTX0; 9 sites.
DR BioMuta; TAS1R3; -.
DR DMDM; 62299063; -.
DR jPOST; Q7RTX0; -.
DR MassIVE; Q7RTX0; -.
DR PaxDb; Q7RTX0; -.
DR PeptideAtlas; Q7RTX0; -.
DR PRIDE; Q7RTX0; -.
DR Antibodypedia; 26289; 331 antibodies from 30 providers.
DR DNASU; 83756; -.
DR Ensembl; ENST00000339381.6; ENSP00000344411.5; ENSG00000169962.5.
DR GeneID; 83756; -.
DR KEGG; hsa:83756; -.
DR MANE-Select; ENST00000339381.6; ENSP00000344411.5; NM_152228.3; NP_689414.2.
DR UCSC; uc010nyk.3; human.
DR CTD; 83756; -.
DR DisGeNET; 83756; -.
DR GeneCards; TAS1R3; -.
DR HGNC; HGNC:15661; TAS1R3.
DR HPA; ENSG00000169962; Tissue enhanced (epididymis, salivary gland).
DR MIM; 605865; gene.
DR neXtProt; NX_Q7RTX0; -.
DR OpenTargets; ENSG00000169962; -.
DR PharmGKB; PA38012; -.
DR VEuPathDB; HostDB:ENSG00000169962; -.
DR eggNOG; KOG1056; Eukaryota.
DR GeneTree; ENSGT00940000160679; -.
DR HOGENOM; CLU_005389_1_0_1; -.
DR InParanoid; Q7RTX0; -.
DR OMA; MEYDLKL; -.
DR OrthoDB; 342693at2759; -.
DR PhylomeDB; Q7RTX0; -.
DR TreeFam; TF331269; -.
DR PathwayCommons; Q7RTX0; -.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR BioGRID-ORCS; 83756; 14 hits in 1062 CRISPR screens.
DR GeneWiki; TAS1R3; -.
DR GenomeRNAi; 83756; -.
DR Pharos; Q7RTX0; Tbio.
DR PRO; PR:Q7RTX0; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q7RTX0; protein.
DR Bgee; ENSG00000169962; Expressed in bone marrow cell and 94 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IC:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:1903767; C:sweet taste receptor complex; IDA:BHF-UCL.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0008527; F:taste receptor activity; IPI:UniProtKB.
DR GO; GO:0001582; P:detection of chemical stimulus involved in sensory perception of sweet taste; IDA:HGNC-UCL.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IC:HGNC-UCL.
DR GO; GO:0050916; P:sensory perception of sweet taste; IDA:UniProtKB.
DR GO; GO:0050917; P:sensory perception of umami taste; IDA:UniProtKB.
DR Gene3D; 2.10.50.30; -; 1.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR000337; GPCR_3.
DR InterPro; IPR011500; GPCR_3_9-Cys_dom.
DR InterPro; IPR038550; GPCR_3_9-Cys_sf.
DR InterPro; IPR017978; GPCR_3_C.
DR InterPro; IPR000068; GPCR_3_Ca_sens_rcpt-rel.
DR InterPro; IPR017979; GPCR_3_CS.
DR InterPro; IPR028082; Peripla_BP_I.
DR PANTHER; PTHR24061; PTHR24061; 1.
DR Pfam; PF00003; 7tm_3; 1.
DR Pfam; PF01094; ANF_receptor; 1.
DR Pfam; PF07562; NCD3G; 1.
DR PRINTS; PR00248; GPCRMGR.
DR SUPFAM; SSF53822; SSF53822; 1.
DR PROSITE; PS00980; G_PROTEIN_RECEP_F3_2; 1.
DR PROSITE; PS50259; G_PROTEIN_RECEP_F3_4; 1.
PE 1: Evidence at protein level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Sensory transduction; Signal; Taste;
KW Transducer; Transmembrane; Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..852
FT /note="Taste receptor type 1 member 3"
FT /id="PRO_0000012961"
FT TOPO_DOM 21..570
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 571..591
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 592..603
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 604..624
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 625..639
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 640..660
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 661..682
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 683..703
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 704..729
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 730..750
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 751..762
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 763..783
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 784..789
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 790..810
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 811..852
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 536..545
FT /note="Required for brazzein responsiveness"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 130
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 264
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 285
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 380
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 411
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 432
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 475
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 757
FT /note="C -> R (in dbSNP:rs307377)"
FT /id="VAR_020788"
FT MUTAGEN 537
FT /note="A->G: Retains partial activity toward brazzein;
FT however response to other sweeteners tested is suppressed."
FT /evidence="ECO:0000269|PubMed:15299024"
FT MUTAGEN 537
FT /note="A->P: Receptor unresponsive to all sweeteners
FT tested."
FT /evidence="ECO:0000269|PubMed:15299024"
FT MUTAGEN 537
FT /note="A->T,S,E,V: Abolished the response to brazzein."
FT /evidence="ECO:0000269|PubMed:15299024"
FT MUTAGEN 540
FT /note="F->A,H: Reduces the response to brazzein and
FT monellin."
FT /evidence="ECO:0000269|PubMed:15299024"
FT MUTAGEN 540
FT /note="F->L: Reduces the response to monellin."
FT /evidence="ECO:0000269|PubMed:15299024"
FT MUTAGEN 540
FT /note="F->Y,P: Reduces the response to brazzein; P-540 also
FT enhances responses to the small molecule sweeteners."
FT /evidence="ECO:0000269|PubMed:15299024"
FT CONFLICT 852
FT /note="E -> EAPILHPARHHSSGRPGGGTQAYAQGRARRGRGGTQLPTLPFAQWVD
FT SIMTVTSTRDRAQDLTPARRQAHTGSPVRVPGSAPSRA (in Ref. 1;
FT BAC05873)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 852 AA; 93386 MW; 9690DEC23E75EB3E CRC64;
MLGPAVLGLS LWALLHPGTG APLCLSQQLR MKGDYVLGGL FPLGEAEEAG LRSRTRPSSP
VCTRFSSNGL LWALAMKMAV EEINNKSDLL PGLRLGYDLF DTCSEPVVAM KPSLMFLAKA
GSRDIAAYCN YTQYQPRVLA VIGPHSSELA MVTGKFFSFF LMPQVSYGAS MELLSARETF
PSFFRTVPSD RVQLTAAAEL LQEFGWNWVA ALGSDDEYGR QGLSIFSALA AARGICIAHE
GLVPLPRADD SRLGKVQDVL HQVNQSSVQV VLLFASVHAA HALFNYSISS RLSPKVWVAS
EAWLTSDLVM GLPGMAQMGT VLGFLQRGAQ LHEFPQYVKT HLALATDPAF CSALGEREQG
LEEDVVGQRC PQCDCITLQN VSAGLNHHQT FSVYAAVYSV AQALHNTLQC NASGCPAQDP
VKPWQLLENM YNLTFHVGGL PLRFDSSGNV DMEYDLKLWV WQGSVPRLHD VGRFNGSLRT
ERLKIRWHTS DNQKPVSRCS RQCQEGQVRR VKGFHSCCYD CVDCEAGSYR QNPDDIACTF
CGQDEWSPER STRCFRRRSR FLAWGEPAVL LLLLLLSLAL GLVLAALGLF VHHRDSPLVQ
ASGGPLACFG LVCLGLVCLS VLLFPGQPSP ARCLAQQPLS HLPLTGCLST LFLQAAEIFV
ESELPLSWAD RLSGCLRGPW AWLVVLLAML VEVALCTWYL VAFPPEVVTD WHMLPTEALV
HCRTRSWVSF GLAHATNATL AFLCFLGTFL VRSQPGCYNR ARGLTFAMLA YFITWVSFVP
LLANVQVVLR PAVQMGALLL CVLGILAAFH LPRCYLLMRQ PGLNTPEFFL GGGPGDAQGQ
NDGNTGNQGK HE
