ID   TSA4_GIAIN              Reviewed;         713 AA.
AC   P21849;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Major surface-labeled trophozoite antigen 417;
DE   Flags: Precursor;
GN   Name=TSA 417;
OS   Giardia intestinalis (Giardia lamblia).
OC   Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX   NCBI_TaxID=5741;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=ATCC 30957 / WB;
RX   PubMed=2352929; DOI=10.1073/pnas.87.12.4463;
RA   Gillin F.D., Hagblom P., Harwood J., Aley S.B., Reiner D.S., McCaffery M.,
RA   So M., Guiney D.G.;
RT   "Isolation and expression of the gene for a major surface protein of
RT   Giardia lamblia.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:4463-4467(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 480-620.
RC   STRAIN=Adelaide-1;
RX   PubMed=8325510; DOI=10.1016/0378-1119(93)90276-9;
RA   Ey P.L., Mayrhofer G.;
RT   "Two genes encoding homologous 70-kDa surface proteins are present within
RT   individual trophozoites of the binucleate protozoan parasite Giardia
RT   intestinalis.";
RL   Gene 129:257-262(1993).
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane
CC       protein.
CC   -!- DOMAIN: Contains 29 repeats of the CXXC motif.
CC   -!- SIMILARITY: Belongs to the Giardia variant surface protein family.
CC       {ECO:0000305}.
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DR   EMBL; M33641; AAA02688.1; -; mRNA.
DR   EMBL; M97488; AAA02581.1; -; Genomic_DNA.
DR   PIR; A35502; A35502.
DR   PIR; PC1294; PC1294.
DR   AlphaFoldDB; P21849; -.
DR   PRIDE; P21849; -.
DR   VEuPathDB; GiardiaDB:DHA2_151647; -.
DR   VEuPathDB; GiardiaDB:GL50581_2080; -.
DR   VEuPathDB; GiardiaDB:GL50581_3480; -.
DR   VEuPathDB; GiardiaDB:GL50803_00113797; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   CDD; cd00064; FU; 1.
DR   InterPro; IPR000742; EGF-like_dom.
DR   InterPro; IPR006212; Furin_repeat.
DR   InterPro; IPR005127; Giardia_VSP.
DR   InterPro; IPR009030; Growth_fac_rcpt_cys_sf.
DR   Pfam; PF03302; VSP; 2.
DR   SMART; SM00181; EGF; 4.
DR   SMART; SM00261; FU; 6.
DR   SUPFAM; SSF57184; SSF57184; 3.
PE   2: Evidence at transcript level;
KW   Cell membrane; Glycoprotein; Membrane; Repeat; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..17
FT   CHAIN           18..713
FT                   /note="Major surface-labeled trophozoite antigen 417"
FT                   /id="PRO_0000036459"
FT   TOPO_DOM        18..679
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        680..708
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        709..713
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        289
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        676
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VARIANT         582
FT                   /note="A -> T (in strain: Adelaide-1)"
FT   VARIANT         606
FT                   /note="A -> S (in strain: Adelaide-1)"
SQ   SEQUENCE   713 AA;  72511 MW;  9AD7195843DE5601 CRC64;
     MFGRFLLAIV ILQLARTACT QEADDGKCKT CGVTIGQDTW CSECNGANYA PVNGQCVDVN
     AEGPSKTLCP QHSAGKCTQC GGNSFMYKDG CYSSGEGLPG HSLCLSSDGD GVCTEAAPGY
     FAPVGAANTE QSVIACGDTT GVTIAAGGNT YKGIADCAEC SAPDATAGAE AGKVATCTKC
     GVSKYLKDNV CVDKAQCNSG STNKFVAVDD SENGNKCVSC SDNLNGGVAN CDTCSYDEQS
     KKIKCTKCTD NNYLKTTSEG TSCVQKDQCK DGFFPKDDSS AGNKCLPCND STDGIANCAT
     CALVSGRSGA ALVTCSACTD GYKPSADKTT CEAVSNCKTP GCKACSNEGK ENEVCTDCDG
     STYLTPTSQC IDSCAKIGNY YGATEGAKKL CKECTAANCK TCDDQGQCQA CNDGFYKNGD
     ACSPCHESCK TCSAGTASDC TECPTGKALR YGDDGTKGTC GEGCTTGTGA GACKTCGLTI
     DGASYCSECA TTTEYPQNGV CAPKASRATP TCNDSPIQNG VCGTCADNYF KMNGGCYETV
     KYPGKTVCIS APNGGTCQKA ADGYKLDSGT LTVCSEGCKE CASSTDCTTC LDGYVKSASA
     CTKCDASCET CNGAATTCKA CATGYYKTAS GEGACTSCES DSNGVTGIKG CLNCAPPPNN
     KGSVLCYLIK DSGSTNKSGL STGAIAGISV AVIVVVGGLI GFLCWWFLCR GKA