ID   TSAB_BUCAI              Reviewed;         221 AA.
AC   P57409;
DT   02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein TsaB;
DE   AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB;
GN   Name=tsaB; OrderedLocusNames=BU324;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Required for the formation of a threonylcarbamoyl group on
CC       adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning
CC       with adenine. Is involved in the transfer of the threonylcarbamoyl
CC       moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37,
CC       together with TsaD and TsaE; this reaction does not require ATP in
CC       vitro. TsaB seems to play an indirect role in the t(6)A biosynthesis
CC       pathway, possibly in regulating the core enzymatic function of TsaD (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KAE1 / TsaD family. TsaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BA000003; BAB13032.1; -; Genomic_DNA.
DR   RefSeq; NP_240146.1; NC_002528.1.
DR   RefSeq; WP_010896067.1; NC_002528.1.
DR   AlphaFoldDB; P57409; -.
DR   SMR; P57409; -.
DR   STRING; 107806.10038998; -.
DR   EnsemblBacteria; BAB13032; BAB13032; BAB13032.
DR   KEGG; buc:BU324; -.
DR   PATRIC; fig|107806.10.peg.336; -.
DR   eggNOG; COG1214; Bacteria.
DR   HOGENOM; CLU_064886_2_0_6; -.
DR   OMA; FIGVRIG; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002949; P:tRNA threonylcarbamoyladenosine modification; IEA:InterPro.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR000905; Gcp-like_dom.
DR   InterPro; IPR022496; T6A_TsaB.
DR   PANTHER; PTHR11735; PTHR11735; 1.
DR   PANTHER; PTHR11735:SF11; PTHR11735:SF11; 1.
DR   Pfam; PF00814; TsaD; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR03725; T6A_YeaZ; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..221
FT                   /note="tRNA threonylcarbamoyladenosine biosynthesis protein
FT                   TsaB"
FT                   /id="PRO_0000096992"
SQ   SEQUENCE   221 AA;  25662 MW;  64820458EA67B222 CRC64;
     MSNIILSIES SLDCCSVAIY KNEYIHSLSE KCKKKHTTHI LPMIKEILSQ TKTEFKELNY
     VSFSKGPGNF TSIRIAASIA QSLSISLKIP IISVSTLAIM AEKTFRKYKQ KDVIVAIHAK
     KKQVYWAKYT RNKNSIWIGE YTESLLEKKI IQEKIENLKK RWTLVSDQSE LIEFQNILNV
     KKNYVFLPNA KDIIPFVLLK IKNKNKSFSI ENNINYLYNQ F