ID   TSAB_BUCAP              Reviewed;         210 AA.
AC   Q8K9L9;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein TsaB;
DE   AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB;
GN   Name=tsaB; OrderedLocusNames=BUsg_315;
OS   Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=198804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sg;
RX   PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Required for the formation of a threonylcarbamoyl group on
CC       adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning
CC       with adenine. Is involved in the transfer of the threonylcarbamoyl
CC       moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37,
CC       together with TsaD and TsaE; this reaction does not require ATP in
CC       vitro. TsaB seems to play an indirect role in the t(6)A biosynthesis
CC       pathway, possibly in regulating the core enzymatic function of TsaD (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KAE1 / TsaD family. TsaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE013218; AAM67869.1; -; Genomic_DNA.
DR   RefSeq; WP_011053836.1; NC_004061.1.
DR   AlphaFoldDB; Q8K9L9; -.
DR   SMR; Q8K9L9; -.
DR   STRING; 198804.BUsg_315; -.
DR   EnsemblBacteria; AAM67869; AAM67869; BUsg_315.
DR   KEGG; bas:BUsg_315; -.
DR   eggNOG; COG1214; Bacteria.
DR   HOGENOM; CLU_064886_2_0_6; -.
DR   OMA; FIGVRIG; -.
DR   OrthoDB; 1826274at2; -.
DR   Proteomes; UP000000416; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002949; P:tRNA threonylcarbamoyladenosine modification; IEA:InterPro.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR000905; Gcp-like_dom.
DR   InterPro; IPR022496; T6A_TsaB.
DR   PANTHER; PTHR11735; PTHR11735; 1.
DR   PANTHER; PTHR11735:SF11; PTHR11735:SF11; 1.
DR   Pfam; PF00814; TsaD; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR03725; T6A_YeaZ; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..210
FT                   /note="tRNA threonylcarbamoyladenosine biosynthesis protein
FT                   TsaB"
FT                   /id="PRO_0000096993"
SQ   SEQUENCE   210 AA;  24122 MW;  CC92F0C8113A8342 CRC64;
     MSDIILAIDT SIDHCSVAVY KKKVIYSLSE NCKKEHTIKI LPMIKKVLMN AKITLKDLNY
     VAFSKGPGKF TGIRISIGIA QSLSLSLKIP IFGISTLSIL AQKAWRKYKK KQILVAVNAK
     IGQVYWGEYL RNNTLLWTGE KTESLIKINK IEDKMKKLKK KWILVGDGWK KIKRENSLKF
     EQKKILYPNA KDIIPFLFSE NSKPRILSFF