ID   TSAB_HAEIN              Reviewed;         236 AA.
AC   P43990;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein TsaB;
DE   AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB;
GN   Name=tsaB; OrderedLocusNames=HI_0388;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=10675023;
RX   DOI=10.1002/(sici)1522-2683(20000101)21:2<411::aid-elps411>3.0.co;2-4;
RA   Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C.,
RA   Fountoulakis M.;
RT   "Two-dimensional map of the proteome of Haemophilus influenzae.";
RL   Electrophoresis 21:411-429(2000).
CC   -!- FUNCTION: Required for the formation of a threonylcarbamoyl group on
CC       adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning
CC       with adenine. Is involved in the transfer of the threonylcarbamoyl
CC       moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37,
CC       together with TsaD and TsaE; this reaction does not require ATP in
CC       vitro. TsaB seems to play an indirect role in the t(6)A biosynthesis
CC       pathway, possibly in regulating the core enzymatic function of TsaD (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KAE1 / TsaD family. TsaB subfamily.
CC       {ECO:0000305}.
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DR   EMBL; L42023; AAC22046.1; -; Genomic_DNA.
DR   PIR; H64006; H64006.
DR   RefSeq; NP_438549.1; NC_000907.1.
DR   RefSeq; WP_005693782.1; NC_000907.1.
DR   AlphaFoldDB; P43990; -.
DR   SMR; P43990; -.
DR   STRING; 71421.HI_0388; -.
DR   EnsemblBacteria; AAC22046; AAC22046; HI_0388.
DR   KEGG; hin:HI_0388; -.
DR   PATRIC; fig|71421.8.peg.406; -.
DR   eggNOG; COG1214; Bacteria.
DR   HOGENOM; CLU_064886_2_0_6; -.
DR   OMA; KRILPMI; -.
DR   PhylomeDB; P43990; -.
DR   BioCyc; HINF71421:G1GJ1-403-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0002949; P:tRNA threonylcarbamoyladenosine modification; IEA:InterPro.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR000905; Gcp-like_dom.
DR   InterPro; IPR022496; T6A_TsaB.
DR   PANTHER; PTHR11735; PTHR11735; 1.
DR   PANTHER; PTHR11735:SF11; PTHR11735:SF11; 1.
DR   Pfam; PF00814; TsaD; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR03725; T6A_YeaZ; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome; tRNA processing.
FT   CHAIN           1..236
FT                   /note="tRNA threonylcarbamoyladenosine biosynthesis protein
FT                   TsaB"
FT                   /id="PRO_0000096991"
SQ   SEQUENCE   236 AA;  26084 MW;  79E8D313BF877D97 CRC64;
     MQNLTLLALD TSTEACSVAL LYRGEKTHIN ELAQRTHTKR ILPMIDEILA NSGLGLNQVD
     ALAFGRGPGS FTGVRVGAGI AQGLAFGADL PVIPISNLTA MAQAAFELHQ AENVVAAIDA
     RMNEVYFSQV VREKVRSDFG EFFQWREIIS EQVCSPEQAI NQLQNDNAFR VGTGWAAYSQ
     FTEKNLTGSD IALPNALYML ELAQVEYLQK HTISALEIEP IYLRNEVTWK KLPGRE