ID   TSAG_ORITS              Reviewed;         524 AA.
AC   P22940;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=56 kDa type-specific antigen;
DE            Short=TSA;
DE   AltName: Full=56 kDa scrub typhus antigen;
DE   AltName: Full=STA56;
DE   AltName: Full=TSG56;
DE   Flags: Precursor;
OS   Orientia tsutsugamushi (Rickettsia tsutsugamushi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Orientia.
OX   NCBI_TaxID=784;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Gilliam;
RX   PubMed=2401407; DOI=10.1016/0378-1119(90)90171-m;
RA   Ohashi N., Nashimoto H., Ikeda H., Tamura A.;
RT   "Cloning and sequencing of the gene (tsg56) encoding a type-specific
RT   antigen from Rickettsia tsutsugamushi.";
RL   Gene 91:119-122(1990).
RN   [2]
RP   PROTEIN SEQUENCE OF 23-57.
RC   STRAIN=Gilliam;
RX   PubMed=2496028; DOI=10.1128/iai.57.5.1427-1431.1989;
RA   Ohashi N., Tamura A., Ohta M., Hayashi K.;
RT   "Purification and partial characterization of a type-specific antigen of
RT   Rickettsia tsutsugamushi.";
RL   Infect. Immun. 57:1427-1431(1989).
CC   -!- FUNCTION: May be an adherent factor for rickettsial adsorption to the
CC       host-cell surface and a determinant of virulence of individual
CC       rickettsial strain. It is the major outer membrane protein.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; JQ0683; JQ0683.
DR   AlphaFoldDB; P22940; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR011250; OMP/PagP_b-brl.
DR   InterPro; IPR004933; TSA.
DR   Pfam; PF03249; TSA; 1.
DR   PRINTS; PR01707; 56KDTSANTIGN.
DR   SUPFAM; SSF56925; SSF56925; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Membrane; Signal; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000269|PubMed:2496028"
FT   CHAIN           23..524
FT                   /note="56 kDa type-specific antigen"
FT                   /id="PRO_0000022591"
FT   TRANSMEM        67..87
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        472..492
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          112..132
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          387..422
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        387..402
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   524 AA;  56057 MW;  782B6273CF392D26 CRC64;
     MKKIMLIASA MSALSLPFSA SAIELGEEGG LECGPYGKVG IVGGMITGAE STRLDSTDSE
     GKKHLSLTTG LPFGGTLAAG MTIAPGFRAE LGVMYLRNIS AEVEVGKGKV DSKGEIKADS
     GGGTDTPIRK RFKLTPPQPT IMPISIADRD VGVDTDILAQ AAAGQPQLTV EQRAADRIAW
     LKNYAGIDYM VPDPQNPNAR VINPVLLNIT QGPPNVQPRP RQNLDILDHG QWRHLVVGVT
     ALSHANKPSV TPVKVLSDKI TKIYSDIKPF ADIAGIDVPD TGLPNSASVE QIQSKMQELN
     DVLEDLRDSF DGYMGNAFAN QIQLNFVMPQ QAQQQQGQGQ QQQAQATAQE AVAAAAVRLL
     NGNDQIAQLY KDLVKLQRHA GVKKAMEKLA AQQEEDAKNQ GEGDCKQQQG ASEKSKEGKG
     KETEFDLSMI VGQVKLYADL FTTESFSIYA GVGAGLAHTY GKIDDKDIKG HTGMVASGAL
     GVAINAAEGV YVDLEGSYMH SFSKIEEKYS INPLMASVGV RYNF