ID   TSAP1_BOVIN             Reviewed;         287 AA.
AC   Q1RMJ7;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=tRNA selenocysteine 1-associated protein 1;
DE   AltName: Full=tRNA selenocysteine-associated protein 1;
GN   Name=TRNAU1AP; Synonyms=TRSPAP1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Heart ventricle;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the early steps of selenocysteine biosynthesis
CC       and tRNA(Sec) charging to the later steps resulting in the
CC       cotranslational incorporation of selenocysteine into selenoproteins.
CC       Stabilizes the SECISBP2, EEFSEC and tRNA(Sec) complex. May be involved
CC       in the methylation of tRNA(Sec). Enhances efficiency of selenoproteins
CC       synthesis (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the tRNA(Sec) complex composed at least of
CC       EEFSEC, SECISBP2, SEPHS1, SEPSECS, TRNAU1AP and tRNA(Sec). Found in a
CC       complex with tRNA(Sec). Interacts with SEPSECS. Associates with mRNP
CC       and/or polysomes. Found in a complex with EEFSEC, SECISBP2, TRNAU1AP
CC       and tRNA(Sec) (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC       Note=Abundant in the nucleus. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RRM TRSPAP family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC114858; AAI14859.1; -; mRNA.
DR   RefSeq; NP_001069435.1; NM_001075967.1.
DR   AlphaFoldDB; Q1RMJ7; -.
DR   SMR; Q1RMJ7; -.
DR   STRING; 9913.ENSBTAP00000013718; -.
DR   PaxDb; Q1RMJ7; -.
DR   PRIDE; Q1RMJ7; -.
DR   Ensembl; ENSBTAT00000013718; ENSBTAP00000013718; ENSBTAG00000010393.
DR   GeneID; 532582; -.
DR   KEGG; bta:532582; -.
DR   CTD; 54952; -.
DR   VEuPathDB; HostDB:ENSBTAG00000010393; -.
DR   VGNC; VGNC:52838; TRNAU1AP.
DR   eggNOG; KOG0118; Eukaryota.
DR   GeneTree; ENSGT00940000156139; -.
DR   HOGENOM; CLU_016304_3_0_1; -.
DR   InParanoid; Q1RMJ7; -.
DR   OMA; LKICNAV; -.
DR   OrthoDB; 775799at2759; -.
DR   TreeFam; TF313275; -.
DR   Proteomes; UP000009136; Chromosome 2.
DR   Bgee; ENSBTAG00000010393; Expressed in semen and 108 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR   GO; GO:0001514; P:selenocysteine incorporation; IBA:GO_Central.
DR   CDD; cd12612; RRM2_SECp43; 1.
DR   Gene3D; 3.30.70.330; -; 2.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR034510; SECp43_RRM2.
DR   InterPro; IPR040434; TSAP1.
DR   InterPro; IPR041085; TSAP1_C.
DR   PANTHER; PTHR37457; PTHR37457; 1.
DR   Pfam; PF00076; RRM_1; 2.
DR   Pfam; PF17654; Trnau1ap; 1.
DR   SMART; SM00360; RRM; 2.
DR   SUPFAM; SSF54928; SSF54928; 1.
DR   PROSITE; PS50102; RRM; 2.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Protein biosynthesis; Reference proteome; Repeat;
KW   RNA-binding.
FT   CHAIN           1..287
FT                   /note="tRNA selenocysteine 1-associated protein 1"
FT                   /id="PRO_0000304916"
FT   DOMAIN          3..86
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          96..175
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
SQ   SEQUENCE   287 AA;  32513 MW;  5D15AA838D625B65 CRC64;
     MAASLWMGDL EPYMDENFIS RAFATMGETV MSVKIIRNRL TGIPAGYCFV EFADLATAEK
     CLHKINGKPL PGATPAKRFK LNYATYGKQP DNSPEYSLFV GDLTPDVDDG MLYEFFVKVY
     PSCRGGKVVL DQTGVSKGYG FVKFTDELEQ KRALTECQGA IGLGSKPVRL SVAIPKASRV
     KPVEYSQMYS YSYNQYYQQY QNYYAQWGYD QNTGSYSYSY PQYGYTQSTM QTYEEVGDDA
     LEDPMPQLDV TEANKEFMEQ SEELYDALMD CHWQPLDTVS SEIPAMM