ID   TSAP1_RAT               Reviewed;         287 AA.
AC   Q9QZI7;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=tRNA selenocysteine 1-associated protein 1;
DE   AltName: Full=SECp43;
DE   AltName: Full=tRNA selenocysteine-associated protein 1;
GN   Name=Trnau1ap; Synonyms=Secp43, Trspap1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION IN A COMPLEX WITH TRNA(SEC),
RP   INTERACTION WITH SEPSECS, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Cerebellum;
RX   PubMed=10606267; DOI=10.1017/s1355838299991598;
RA   Ding F., Grabowski P.J.;
RT   "Identification of a protein component of a mammalian tRNA(Sec) complex
RT   implicated in the decoding of UGA as selenocysteine.";
RL   RNA 5:1561-1569(1999).
CC   -!- FUNCTION: Involved in the early steps of selenocysteine biosynthesis
CC       and tRNA(Sec) charging to the later steps resulting in the
CC       cotranslational incorporation of selenocysteine into selenoproteins.
CC       Stabilizes the SECISBP2, EEFSEC and tRNA(Sec) complex. May be involved
CC       in the methylation of tRNA(Sec). Enhances efficiency of selenoproteins
CC       synthesis (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the tRNA(Sec) complex composed at least of
CC       EEFSEC, SECISBP2, SEPHS1, SEPSECS, TRNAU1AP and tRNA(Sec). Associates
CC       with mRNP and/or polysomes (By similarity). Found in a complex with
CC       tRNA(Sec). Interacts with SEPSECS. {ECO:0000250,
CC       ECO:0000269|PubMed:10606267}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10606267}. Cytoplasm
CC       {ECO:0000269|PubMed:10606267}. Note=Abundant in the nucleus.
CC   -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:10606267}.
CC   -!- SIMILARITY: Belongs to the RRM TRSPAP family. {ECO:0000305}.
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DR   EMBL; AF181856; AAD54419.1; -; mRNA.
DR   RefSeq; NP_075416.1; NM_023027.1.
DR   AlphaFoldDB; Q9QZI7; -.
DR   SMR; Q9QZI7; -.
DR   STRING; 10116.ENSRNOP00000065702; -.
DR   PaxDb; Q9QZI7; -.
DR   Ensembl; ENSRNOT00000113510; ENSRNOP00000084120; ENSRNOG00000055344.
DR   GeneID; 65241; -.
DR   KEGG; rno:65241; -.
DR   CTD; 54952; -.
DR   RGD; 619995; Trnau1ap.
DR   eggNOG; KOG0118; Eukaryota.
DR   GeneTree; ENSGT00940000156139; -.
DR   InParanoid; Q9QZI7; -.
DR   OrthoDB; 775799at2759; -.
DR   PhylomeDB; Q9QZI7; -.
DR   PRO; PR:Q9QZI7; -.
DR   Proteomes; UP000002494; Chromosome 5.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; ISS:HGNC-UCL.
DR   GO; GO:0000049; F:tRNA binding; IDA:RGD.
DR   GO; GO:0001514; P:selenocysteine incorporation; ISS:HGNC-UCL.
DR   CDD; cd12612; RRM2_SECp43; 1.
DR   Gene3D; 3.30.70.330; -; 2.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR034510; SECp43_RRM2.
DR   InterPro; IPR040434; TSAP1.
DR   InterPro; IPR041085; TSAP1_C.
DR   PANTHER; PTHR37457; PTHR37457; 1.
DR   Pfam; PF00076; RRM_1; 2.
DR   Pfam; PF17654; Trnau1ap; 1.
DR   SMART; SM00360; RRM; 2.
DR   SUPFAM; SSF54928; SSF54928; 1.
DR   PROSITE; PS50102; RRM; 2.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Protein biosynthesis; Reference proteome; Repeat;
KW   RNA-binding.
FT   CHAIN           1..287
FT                   /note="tRNA selenocysteine 1-associated protein 1"
FT                   /id="PRO_0000304920"
FT   DOMAIN          3..86
FT                   /note="RRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   DOMAIN          96..175
FT                   /note="RRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
SQ   SEQUENCE   287 AA;  32454 MW;  5485AE4CB30E56F5 CRC64;
     MAASLWMGDL EPYMDENFIS RAFATMGETV MSVKIIRNRL TGIPAGYCFV EFADLATAEK
     CLHKINGKPL PGATPAKRFK LNYATYGKQP DNSPEYSLFV GDLTPDVDDG MLYEFFVKVY
     PSCRGGKVVL DQTGVSKGYG FVKFTDELEQ KRALTECQGA VGLGCKPVRL SVAIPKASRV
     KPVEYSQMYS YSYNQYYQQY QNYYAQWGYD QNTGSYSYSY PQYGYTQSTM QTYEEVGDDA
     LEDPAPQLDV TEANKEFMEQ SEELYDALMD CHWQPLDTVS SEIPAMM