ID   TSAQ_COMTE              Reviewed;         278 AA.
AC   Q6XL52;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=HTH-type transcriptional regulator TsaQ1/TsaQ2;
GN   Name=tsaQ1;
GN   and
GN   Name=tsaQ2;
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OG   Plasmid pTSA.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 6577 / T-2;
RX   PubMed=11282598; DOI=10.1128/aem.67.4.1508-1516.2001;
RA   Tralau T., Cook A.M., Ruff J.;
RT   "Map of the IncP1beta plasmid pTSA encoding the widespread genes (tsa) for
RT   p-toluenesulfonate degradation in Comamonas testosteroni T-2.";
RL   Appl. Environ. Microbiol. 67:1508-1516(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=DSM 6577 / T-2;
RX   PubMed=13680097; DOI=10.1007/s00203-003-0594-8;
RA   Tralau T., Cook A.M., Ruff J.;
RT   "An additional regulator, TsaQ, is involved with TsaR in regulation of
RT   transport during the degradation of p-toluenesulfonate in Comamonas
RT   testosteroni T-2.";
RL   Arch. Microbiol. 180:319-326(2003).
CC   -!- FUNCTION: Both copies function as additional regulators for the tsa
CC       locus, specifically for tsaT. {ECO:0000269|PubMed:13680097}.
CC   -!- INDUCTION: Induced by p-toluenesulfonate (TSA), p-toluenecarboxylate
CC       (TCA) and p-sulfobenzoate (PSB). {ECO:0000269|PubMed:13680097}.
CC   -!- DISRUPTION PHENOTYPE: When both copies are inactivated, the mutants are
CC       unable to grow with TSA, but show normal growth with TCA. Growth with
CC       PSB is slightly faster than growth of the wild-type strain.
CC       {ECO:0000269|PubMed:13680097}.
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DR   EMBL; AF311437; AAP69991.1; -; Genomic_DNA.
DR   EMBL; AF311437; AAP69994.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6XL52; -.
DR   SMR; Q6XL52; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.450.40; -; 1.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR014757; Tscrpt_reg_IclR_C.
DR   InterPro; IPR005471; Tscrpt_reg_IclR_N.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF09339; HTH_IclR; 1.
DR   SMART; SM00346; HTH_ICLR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS51077; HTH_ICLR; 1.
DR   PROSITE; PS51078; ICLR_ED; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Plasmid; Transcription; Transcription regulation.
FT   CHAIN           1..278
FT                   /note="HTH-type transcriptional regulator TsaQ1/TsaQ2"
FT                   /id="PRO_0000430302"
FT   DOMAIN          19..80
FT                   /note="HTH iclR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
FT   DOMAIN          95..266
FT                   /note="IclR-ED"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00394"
FT   DNA_BIND        40..59
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
SQ   SEQUENCE   278 AA;  30460 MW;  B6DEB0961E98C303 CRC64;
     MATHVKTPDQ LQESGTGLVH SLAKGLEILS CFSEGELLGN QQLVELTGLP KATVSRLTST
     LVKLGYLQVD PRSRKLAMGA RVLGLGVSVQ RKLGLQRIAR PHMEALSQRF GLTVTMGTRD
     RLSVVLLEVC RPPSLAQLVV NFDAGTHMPL SQTALGLASL VNSPVKDREQ VIEGLRKQLG
     DQWVEARNRI ERAHQEHERY GYIVSQRSLG RDVSGVAVGM VPMGSNTPYV FHMAGPSNQM
     PLSLMRSDMG PALKQMVQDI QAEMRAARPP KLVVPKEF