ID   TSAS_COMTE              Reviewed;         239 AA.
AC   Q6A553;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Probable inner membrane transporter protein TsaS;
GN   Name=tsaS;
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OG   Plasmid pTSA.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 6577 / T-2;
RX   PubMed=11282598; DOI=10.1128/aem.67.4.1508-1516.2001;
RA   Tralau T., Cook A.M., Ruff J.;
RT   "Map of the IncP1beta plasmid pTSA encoding the widespread genes (tsa) for
RT   p-toluenesulfonate degradation in Comamonas testosteroni T-2.";
RL   Appl. Environ. Microbiol. 67:1508-1516(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, AND INDUCTION.
RC   STRAIN=DSM 6577 / T-2;
RX   PubMed=15176949; DOI=10.1042/bj20040652;
RA   Mampel J., Maier E., Tralau T., Ruff J., Benz R., Cook A.M.;
RT   "A novel outer-membrane anion channel (porin) as part of a putatively two-
RT   component transport system for 4-toluenesulphonate in Comamonas
RT   testosteroni T-2.";
RL   Biochem. J. 383:91-99(2004).
CC   -!- FUNCTION: Involved in the uptake of p-toluenesulphonate (TSA).
CC       {ECO:0000269|PubMed:15176949}.
CC   -!- SUBUNIT: Part of a two-component transport system composed of TsaT and
CC       TsaS. {ECO:0000305|PubMed:15176949}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- INDUCTION: Constitutively expressed. {ECO:0000269|PubMed:15176949}.
CC   -!- SIMILARITY: Belongs to the 4-toluene sulfonate uptake permease (TSUP)
CC       (TC 2.A.102) family. {ECO:0000305}.
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DR   EMBL; AF311437; AAT81376.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6A553; -.
DR   TCDB; 2.A.102.1.1; the 4-toluene sulfonate uptake permease (tsup) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR002781; TM_pro_TauE-like.
DR   Pfam; PF01925; TauE; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Plasmid; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..239
FT                   /note="Probable inner membrane transporter protein TsaS"
FT                   /id="PRO_0000430300"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        128..148
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        186..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   239 AA;  25657 MW;  FBDFB4E4BC4224CA CRC64;
     MAVPRQRAAG GLCGDGHHRF WLGPGGGALL AGSGRCRGGG LVILLDVPAS MLHGGLNFRQ
     VRWRAIGAIL PGMAVGALIG LWLMGQLDKR WPLFLLGLYI TWVGWRTLRH GQQAARALPG
     WTHHAGSGLV GVLEVMFATA GPMVIALLQR RLREVAEIRA TVPVVMVVAA SIAIAVLFGA
     GQIDRAHTFE RWLVALPIAF MGVVLGNRLA RHIPPPAMRR AMAVLLIASG LSLTQHLWR