ID   TSAT_COMTE              Reviewed;         338 AA.
AC   Q8KR68;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 2.
DT   03-AUG-2022, entry version 39.
DE   RecName: Full=Outer membrane transporter protein TsaT;
DE   Flags: Precursor;
GN   Name=tsaT;
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OG   Plasmid pTSA.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 6577 / T-2;
RX   PubMed=11282598; DOI=10.1128/aem.67.4.1508-1516.2001;
RA   Tralau T., Cook A.M., Ruff J.;
RT   "Map of the IncP1beta plasmid pTSA encoding the widespread genes (tsa) for
RT   p-toluenesulfonate degradation in Comamonas testosteroni T-2.";
RL   Appl. Environ. Microbiol. 67:1508-1516(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-29, FUNCTION,
RP   SUBUNIT, SUBCELLULAR LOCATION, AND INDUCTION.
RC   STRAIN=DSM 6577 / T-2;
RX   PubMed=15176949; DOI=10.1042/bj20040652;
RA   Mampel J., Maier E., Tralau T., Ruff J., Benz R., Cook A.M.;
RT   "A novel outer-membrane anion channel (porin) as part of a putatively two-
RT   component transport system for 4-toluenesulphonate in Comamonas
RT   testosteroni T-2.";
RL   Biochem. J. 383:91-99(2004).
RN   [3]
RP   INDUCTION.
RC   STRAIN=DSM 6577 / T-2;
RX   PubMed=13680097; DOI=10.1007/s00203-003-0594-8;
RA   Tralau T., Cook A.M., Ruff J.;
RT   "An additional regulator, TsaQ, is involved with TsaR in regulation of
RT   transport during the degradation of p-toluenesulfonate in Comamonas
RT   testosteroni T-2.";
RL   Arch. Microbiol. 180:319-326(2003).
CC   -!- FUNCTION: Involved in the uptake of p-toluenesulphonate (TSA). Forms a
CC       large, general diffusion pore with a preference for anions.
CC       {ECO:0000269|PubMed:15176949}.
CC   -!- SUBUNIT: Part of a two-component transport system composed of TsaT and
CC       TsaS. {ECO:0000305|PubMed:15176949}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane
CC       {ECO:0000269|PubMed:15176949}.
CC   -!- INDUCTION: Induced by TSA. Transcriptionally regulated by TsaQ and
CC       TsaR. {ECO:0000269|PubMed:13680097, ECO:0000269|PubMed:15176949}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF311437; AAL02394.2; -; Genomic_DNA.
DR   AlphaFoldDB; Q8KR68; -.
DR   SMR; Q8KR68; -.
DR   TCDB; 9.A.56.1.1; the outer membrane anion porin, tsat (tsat) family.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR   GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0071702; P:organic substance transport; IEA:UniProt.
DR   Gene3D; 3.40.190.170; -; 1.
DR   InterPro; IPR018389; DctP_fam.
DR   InterPro; IPR038404; TRAP_DctP_sf.
DR   PANTHER; PTHR33376; PTHR33376; 1.
DR   Pfam; PF03480; DctP; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Direct protein sequencing; Ion transport; Membrane;
KW   Plasmid; Porin; Signal; Transmembrane; Transport.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..338
FT                   /note="Outer membrane transporter protein TsaT"
FT                   /id="PRO_0000430299"
SQ   SEQUENCE   338 AA;  36535 MW;  EF05B7CF61E0EFB1 CRC64;
     MNFRRRLCTA ALIAALPLAS QAQNNTLLLN SVLAPQNPMT KMIVKPWAEK IAQVTEGRVK
     VDVAPSSLAA PQQQLASVNK GVFDIAYQFH GLLTDQVKLN QIAQLPFVNT TARGSSVALW
     RTYQKHFAKA NELGEVQVLA LFVQPPGVMF GMKGPIDGMD KLKGRKVYAL PGVPSAMMES
     AGAAVVAAPG ARSYEIVSGK TVDAFVGYPT SDAEGLKTLS YATDVTDIPG NLTAVSWVLF
     MNKKRWAALS EKDRKAIESI SGEAFAQGMK QYDDLETKVR SEAAAKGIKF HMANDAFVKE
     LQTLATPITQ AWLKDASSRG VNGQEALDFY RAQAAANR