ID   TSAT_ORITS              Reviewed;         529 AA.
AC   P37918;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=56 kDa type-specific antigen;
DE            Short=TSA;
DE   AltName: Full=56 kDa scrub typhus antigen;
DE   AltName: Full=STA56;
DE   AltName: Full=TST56;
DE   Flags: Precursor;
OS   Orientia tsutsugamushi (Rickettsia tsutsugamushi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Orientia.
OX   NCBI_TaxID=784;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Kato;
RX   PubMed=1618776; DOI=10.1016/s0021-9258(18)42337-x;
RA   Ohashi N., Nashimoto H., Ikeda H., Tamura A.;
RT   "Diversity of immunodominant 56-kDa type-specific antigen (TSA) of
RT   Rickettsia tsutsugamushi. Sequence and comparative analyses of the genes
RT   encoding TSA homologues from four antigenic variants.";
RL   J. Biol. Chem. 267:12728-12735(1992).
RN   [2]
RP   PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=Kato;
RX   PubMed=2496028; DOI=10.1128/iai.57.5.1427-1431.1989;
RA   Ohashi N., Tamura A., Ohta M., Hayashi K.;
RT   "Purification and partial characterization of a type-specific antigen of
RT   Rickettsia tsutsugamushi.";
RL   Infect. Immun. 57:1427-1431(1989).
CC   -!- FUNCTION: May be an adherent factor for rickettsial adsorption to the
CC       host-cell surface and a determinant of virulence of individual
CC       rickettsial strain. It is the major outer membrane protein.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
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DR   EMBL; M63382; AAA26397.1; -; Genomic_DNA.
DR   PIR; A42804; A42804.
DR   RefSeq; WP_045916090.1; NZ_LS398550.1.
DR   AlphaFoldDB; P37918; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR011250; OMP/PagP_b-brl.
DR   InterPro; IPR004933; TSA.
DR   Pfam; PF03249; TSA; 1.
DR   PRINTS; PR01707; 56KDTSANTIGN.
DR   SUPFAM; SSF56925; SSF56925; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Membrane; Signal; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   SIGNAL          1..22
FT   CHAIN           23..529
FT                   /note="56 kDa type-specific antigen"
FT                   /id="PRO_0000022595"
FT   TRANSMEM        67..87
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        477..492
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          106..134
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          392..424
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        398..424
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   529 AA;  56115 MW;  F2A09C7C03F7CD68 CRC64;
     MKKIMLIASA MSALSLPFSA SAIELGDEGG LECGPYAKVG VVGGMITGVE STRLDPADAG
     GKKQLPLTTS MPFGGTLAAG MTIAPGFRAE LGVMYLANVK AEVESGKTGS DADIRSGADS
     PMPQRYKLTP PQPTIMPISI ADRDLGVDIP NVPQGGANHL GDNLGANDIR RADDRITWLK
     NYAGVDYMVP DPNNPQARIV NPVLLNIPQG PPNANPRQAM QPCSILNHDH WRHLVVGITA
     MSNANKPSVS PIKVLSEKIV QIYRDVKPFA RVAGIEVPSD PLPNSASVEQ IQNKMQELND
     ILDEIRDSFD GCIGGNAFAN QIQLNFRIPQ AQQQGQGQQQ QQAQATAQEA AAAAAVRVLN
     NNDQIIKLYK DLVKLKRHAG IKKAMEELAA QDGGCNGGGD NKKKRGASED SDAGGASKGG
     KGKETKETEF DLSMIVGQVK LYADLFTTES FSIYAGLGAG LAYTSGKIDG VDIKANTGMV
     ASGALGVAIN AAEGVYVDIE GSYMHSFSKI EEKYSINPLM ASFGVRYNF