TSC3_YEAST
ID TSC3_YEAST Reviewed; 80 AA.
AC Q3E790; D6VQ58;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Serine palmitoyltransferase-regulating protein TSC3;
DE AltName: Full=Temperature-sensitive CSG2-mutant suppressor protein 3;
GN Name=TSC3; OrderedLocusNames=YBR058C-A;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP INTERACTION WITH LCB1 AND LCB2, AND SUBCELLULAR LOCATION.
RX PubMed=10713067; DOI=10.1074/jbc.275.11.7597;
RA Gable K., Slife H., Bacikova D., Monaghan E., Dunn T.M.;
RT "Tsc3p is an 80-amino acid protein associated with serine
RT palmitoyltransferase and required for optimal enzyme activity.";
RL J. Biol. Chem. 275:7597-7603(2000).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP IDENTIFICATION IN THE SPOTS COMPLEX.
RX PubMed=20182505; DOI=10.1038/nature08787;
RA Breslow D.K., Collins S.R., Bodenmiller B., Aebersold R., Simons K.,
RA Shevchenko A., Ejsing C.S., Weissman J.S.;
RT "Orm family proteins mediate sphingolipid homeostasis.";
RL Nature 463:1048-1053(2010).
CC -!- FUNCTION: Stimulates the activity of serine palmitoyltransferase (SPT).
CC -!- SUBUNIT: Interacts with the serine palmitoyltransferase complex LCB1-
CC LCB2. Component of the SPOTS complex, at least composed of LCB1/2 (LCB1
CC and/or LCB2), ORM1/2 (ORM1 and/or ORM2), SAC1 and TSC3.
CC {ECO:0000269|PubMed:10713067, ECO:0000269|PubMed:20182505}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:10713067, ECO:0000269|PubMed:14562095}; Single-pass
CC membrane protein {ECO:0000269|PubMed:10713067,
CC ECO:0000269|PubMed:14562095}.
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DR EMBL; Z35927; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK006936; DAA07178.1; -; Genomic_DNA.
DR RefSeq; NP_116327.3; NM_001184442.3.
DR AlphaFoldDB; Q3E790; -.
DR SMR; Q3E790; -.
DR BioGRID; 32762; 284.
DR ComplexPortal; CPX-3158; SPOTS complex.
DR DIP; DIP-47347N; -.
DR IntAct; Q3E790; 4.
DR STRING; 4932.YBR058C-A; -.
DR iPTMnet; Q3E790; -.
DR MaxQB; Q3E790; -.
DR PaxDb; Q3E790; -.
DR PRIDE; Q3E790; -.
DR EnsemblFungi; YBR058C-A_mRNA; YBR058C-A; YBR058C-A.
DR GeneID; 852350; -.
DR KEGG; sce:YBR058C-A; -.
DR SGD; S000007521; TSC3.
DR VEuPathDB; FungiDB:YBR058C-A; -.
DR eggNOG; ENOG502S91C; Eukaryota.
DR HOGENOM; CLU_176405_0_0_1; -.
DR InParanoid; Q3E790; -.
DR OMA; YYVHLPF; -.
DR BioCyc; YEAST:G3O-29249-MON; -.
DR PRO; PR:Q3E790; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; Q3E790; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0035339; C:SPOTS complex; IDA:UniProtKB.
DR GO; GO:0008047; F:enzyme activator activity; IDA:SGD.
DR GO; GO:0006666; P:3-keto-sphinganine metabolic process; IPI:SGD.
DR GO; GO:0090156; P:cellular sphingolipid homeostasis; IC:ComplexPortal.
DR GO; GO:0090154; P:positive regulation of sphingolipid biosynthetic process; IMP:SGD.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..80
FT /note="Serine palmitoyltransferase-regulating protein TSC3"
FT /id="PRO_0000076358"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 80 AA; 9579 MW; F23EE19603FEC87E CRC64;
MTQHKSSMVY IPTTKEAKRR NGKSEGILNT IEEVVEKLYW TYYIHLPFYL MASFDSFFLH
VFFLTIFSLS FFGILKYCFL
