ID   TSCO1_ANDAU             Reviewed;           4 AA.
AC   C0HLZ5;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 1.
DT   03-AUG-2022, entry version 2.
DE   RecName: Full=Tetrascorpin-1 {ECO:0000303|PubMed:34946686};
DE            Short=AaTs-1 {ECO:0000303|PubMed:34946686};
DE   Flags: Fragment;
OS   Androctonus australis (Sahara scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Androctonus.
OX   NCBI_TaxID=6858;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   MASS SPECTROMETRY.
RX   PubMed=34946686; DOI=10.3390/molecules26247610;
RA   Aissaoui-Zid D., Saada M.C., Moslah W., Potier-Cartereau M., Lemettre A.,
RA   Othman H., Gaysinski M., Abdelkafi-Koubaa Z., Souid S., Marrakchi N.,
RA   Vandier C., Essafi-Benkhadir K., Srairi-Abid N.;
RT   "AaTs-1: A Tetrapeptide from Androctonus australis Scorpion Venom,
RT   Inhibiting U87 glioblastoma cells proliferation by p53 and FPRL-1 up-
RT   regulations.";
RL   Molecules 26:0-0(2021).
CC   -!- FUNCTION: Specific inhibitor of store-operated non-voltage-gated
CC       calcium channels (By similarity). Decreases store-operated Ca2+ entry
CC       and cytosolic calcium concentration in a dose-dependent manner in U87
CC       cells (PubMed:34946686). Exhibits anti-tumoral activity by inhibiting
CC       proliferation of human U87 glioblastoma cells with an IC(50) of 300
CC       ug/mL (PubMed:34946686). Also exhibits anti-tumoral activity towards
CC       human glioblastoma U251 cells, but with lower activity (IC(50)
CC       estimated to be more than 500 ug/mL) (PubMed:34946686). Has no effect
CC       on proliferation of the breast cancer cell line MDA-MB231, colon
CC       adenocarcinoma cell line LS174, and liver cancer cell line HepG2
CC       (PubMed:34946686). Does not induce mortality in mice up to 3.5 ug doses
CC       (PubMed:34946686). {ECO:0000250|UniProtKB:P84464,
CC       ECO:0000269|PubMed:34946686}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:34946686}.
CC   -!- TISSUE SPECIFICITY: Expressed by venom gland.
CC       {ECO:0000305|PubMed:34946686}.
CC   -!- MASS SPECTROMETRY: Mass=533.3158; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:34946686};
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing;
KW   Ion channel impairing toxin; Secreted; Toxin.
FT   PEPTIDE         <1..>4
FT                   /note="Tetrascorpin-1"
FT                   /id="PRO_0000455285"
FT   UNSURE          1
FT                   /note="I or L"
FT                   /evidence="ECO:0000269|PubMed:34946686"
FT   NON_TER         1
FT                   /evidence="ECO:0000269|PubMed:34946686"
FT   NON_TER         4
FT                   /evidence="ECO:0000269|PubMed:34946686"
SQ   SEQUENCE   4 AA;  533 MW;  75B3237030000000 CRC64;
     IWKS