TSCOT_MOUSE
ID TSCOT_MOUSE Reviewed; 479 AA.
AC Q8CA03; A2ANT4; Q9D3J2; Q9JLQ3;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Thymic stromal cotransporter protein;
DE AltName: Full=Solute carrier family 46 member 2;
GN Name=Slc46a2; Synonyms=Tscot;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Thymus;
RX PubMed=10706709; DOI=10.4049/jimmunol.164.6.3185;
RA Kim M.G., Flomerfelt F.A., Lee K.-N., Chen C., Schwartz R.H.;
RT "A putative 12 transmembrane domain cotransporter expressed in thymic
RT cortical epithelial cells.";
RL J. Immunol. 164:3185-3192(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=129;
RX PubMed=10978518; DOI=10.1016/s0167-4781(00)00177-9;
RA Chen C., Kim M.G., Lyu M.S., Kozak C.A., Schwartz R.H., Flomerfelt F.A.;
RT "Characterization of the mouse gene, human promoter and human cDNA of TSCOT
RT reveals strong interspecies homology.";
RL Biochim. Biophys. Acta 1493:159-169(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: May act as a transporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed on cortical epithelial cells in the
CC thymus. Mainly expressed in the thymic cortex and is highly enriched in
CC SCID thymus. Also expressed in lymph nodes, heart, fetal liver, brain,
CC spleen, intestine and kidney, but not in adult liver, skin, skeletal
CC muscle and lung. {ECO:0000269|PubMed:10706709,
CC ECO:0000269|PubMed:10978518}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed during fetal thymus development
CC and decreases after day 16. {ECO:0000269|PubMed:10706709}.
CC -!- PTM: Glycosylated. {ECO:0000269|PubMed:10706709}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. SLC46A
CC family. {ECO:0000305}.
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DR EMBL; AF148145; AAF73110.1; -; mRNA.
DR EMBL; AF242559; AAK28344.1; -; Genomic_DNA.
DR EMBL; AK017363; BAB30710.1; -; mRNA.
DR EMBL; AK040063; BAC30504.1; -; mRNA.
DR EMBL; AL831738; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS18227.1; -.
DR RefSeq; NP_066395.3; NM_021053.4.
DR AlphaFoldDB; Q8CA03; -.
DR SMR; Q8CA03; -.
DR STRING; 10090.ENSMUSP00000030081; -.
DR GlyGen; Q8CA03; 2 sites.
DR PhosphoSitePlus; Q8CA03; -.
DR PaxDb; Q8CA03; -.
DR PRIDE; Q8CA03; -.
DR ProteomicsDB; 298141; -.
DR Antibodypedia; 29714; 59 antibodies from 18 providers.
DR DNASU; 30936; -.
DR Ensembl; ENSMUST00000030081; ENSMUSP00000030081; ENSMUSG00000028386.
DR GeneID; 30936; -.
DR KEGG; mmu:30936; -.
DR UCSC; uc008taf.2; mouse.
DR CTD; 57864; -.
DR MGI; MGI:1353616; Slc46a2.
DR VEuPathDB; HostDB:ENSMUSG00000028386; -.
DR eggNOG; KOG2816; Eukaryota.
DR GeneTree; ENSGT00950000183096; -.
DR HOGENOM; CLU_028365_3_0_1; -.
DR InParanoid; Q8CA03; -.
DR OMA; AYWSGVM; -.
DR OrthoDB; 763423at2759; -.
DR PhylomeDB; Q8CA03; -.
DR TreeFam; TF315701; -.
DR BioGRID-ORCS; 30936; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q8CA03; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8CA03; protein.
DR Bgee; ENSMUSG00000028386; Expressed in tail skin and 26 other tissues.
DR Genevisible; Q8CA03; MM.
DR GO; GO:0009986; C:cell surface; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0070233; P:negative regulation of T cell apoptotic process; IGI:MGI.
DR GO; GO:0070430; P:positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway; ISO:MGI.
DR GO; GO:0045580; P:regulation of T cell differentiation; IGI:MGI.
DR GO; GO:0043029; P:T cell homeostasis; IGI:MGI.
DR GO; GO:0048538; P:thymus development; IGI:MGI.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR001958; Tet-R_TetA/multi-R_MdtG.
DR Pfam; PF07690; MFS_1; 1.
DR PRINTS; PR01035; TCRTETA.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..479
FT /note="Thymic stromal cotransporter protein"
FT /id="PRO_0000065660"
FT TOPO_DOM 1..23
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 24..44
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 45..80
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..110
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 132..140
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..174
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..207
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 229..281
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..321
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 322..342
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 343..348
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 370..371
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..407
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..428
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 429..441
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..479
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 255..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 257..276
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 57
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 61
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 94
FT /note="S -> T (in Ref. 3; BAB30710)"
FT /evidence="ECO:0000305"
FT CONFLICT 274
FT /note="G -> R (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 465
FT /note="V -> I (in Ref. 3; BAC30504)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 479 AA; 52083 MW; EC8BEF1E446A5CFB CRC64;
MGPGGTCPWS SRLSGFRVRT WIEPVVASTQ VAGSLYDAGL LLVVKESFKS EAGGSSNYSA
NQSLVEYQED QQQKAISNFN IIYNLVLGLT PLLSAYGLGW LSDRYHRKIS ICTAMLGFLL
SRIGLLLKVM LDWPVEVMYG AAALNGLCGS FSAYWSGVMA LGSLGCSEGR RSVRLILIDL
VLGLAGFSGS MASGHLFKQI VGHSAQGLLL TACSVGCAAF ALFYSLFVLK VPESKPNKVH
PTVDTVSGMM GTYRTLDPDQ QDKQNVPRNP RTPGKGKSSQ REVVALLFVG AIIYDLAAVG
TVDVMALFVL KEPLHWNQVQ LGYGMASGYI IFITSFLGVL VFSRCFRDTT MIIIGMLSFG
SGALLLAFVK ETYMFYIARA IMLFALIPIT TIRSAMSKLI KDSSYGKIFV ILQLCLTLTG
VVTSTIYNKI YQLTLDKFIG TCFVLSSFLS FLAIVPIGVV AYKQVPRSQQ GECAEKQRS
