ID   TSD2_USTMA              Reviewed;         845 AA.
AC   Q99107; A0A0D1CJA7; Q4P293;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Protein TSD2;
GN   Name=TSD2; ORFNames=UMAG_05770;
OS   Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX   NCBI_TaxID=237631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=UCM3;
RX   PubMed=9037106; DOI=10.1007/s004380050344;
RA   Onel K., Holloman W.K.;
RT   "Molecular cloning of a gene required for DNA replication in Ustilago
RT   maydis.";
RL   Mol. Gen. Genet. 253:463-468(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=17080091; DOI=10.1038/nature05248;
RA   Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA   Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA   Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA   Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA   Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA   Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA   Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA   Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA   Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA   Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA   Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA   Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA   Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA   Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA   Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA   Birren B.W.;
RT   "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT   maydis.";
RL   Nature 444:97-101(2006).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=521 / FGSC 9021;
RA   Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Temperature-sensitive protein required for DNA synthesis. May
CC       be a transcription factor that regulates the level or influences the
CC       stability of DNA polymerases or auxiliary proteins.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Is cell cycle-regulated; expression peaks in early
CC       S or G1 phase.
CC   -!- SIMILARITY: Belongs to the CDC45 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U50276; AAA93459.1; -; Genomic_DNA.
DR   EMBL; CM003155; KIS66988.1; -; Genomic_DNA.
DR   RefSeq; XP_011391503.1; XM_011393201.1.
DR   AlphaFoldDB; Q99107; -.
DR   SMR; Q99107; -.
DR   STRING; 5270.UM05770P0; -.
DR   EnsemblFungi; KIS66988; KIS66988; UMAG_05770.
DR   GeneID; 23565565; -.
DR   KEGG; uma:UMAG_05770; -.
DR   VEuPathDB; FungiDB:UMAG_05770; -.
DR   eggNOG; KOG2475; Eukaryota.
DR   HOGENOM; CLU_005871_3_0_1; -.
DR   InParanoid; Q99107; -.
DR   OMA; PWNLANV; -.
DR   OrthoDB; 1309031at2759; -.
DR   Proteomes; UP000000561; Chromosome 16.
DR   GO; GO:0031261; C:DNA replication preinitiation complex; IBA:GO_Central.
DR   GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR   GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR   GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR   GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR   GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR   GO; GO:1902977; P:mitotic DNA replication preinitiation complex assembly; IBA:GO_Central.
DR   InterPro; IPR003874; CDC45.
DR   PANTHER; PTHR10507; PTHR10507; 1.
DR   Pfam; PF02724; CDC45; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; DNA replication; Nucleus; Reference proteome.
FT   CHAIN           1..845
FT                   /note="Protein TSD2"
FT                   /id="PRO_0000192820"
FT   REGION          193..283
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        193..216
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        227..246
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        248..273
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   845 AA;  92136 MW;  C5AF7BE60FBA5EE6 CRC64;
     MVVVACPNSS LVEAQMDADR SGSCSKYDYA SAYSRLRSAA RAAGTGVSSV LILSSPDVDS
     VCATRILTSL LLQDDIAHRI VPVEGYRTLL NTLSTVFAPH DNDNASSSSS STTDVKSVVF
     INLGAVLSLP TTFNIPPSCT IHVIDSHRPW NLENLFATSH ANDSVWCWDD GEIATKLCRQ
     GGERDAFEKL EFDVDSDSES DSDSHSDSHS DSVSDPEDAS DSNDSGSDSA RSHDERQRDG
     SGGKRKRGSH SPLSRRRQRH KQGQRHKPRH RSTSASATRL TNAERHRYRN ILTRYYARGE
     SFGMSVSSML YLLCESLGRA DRESLWLAIV GLTSLYLSNN IDLETYETYS AAYASEVIAI
     EPTSSNAPTE AWSLDLLTNA NNNAQEQAAR SATATATGAA RAQNRAVRSV ISKNTKAEDA
     DDRSIRIVGS ELRLTLYRHW SLETAMYHTA YVAAKLGIWR ERGLSKLRGL MAKMGFSLTA
     VRQNYTHMPL DLRRSLVRKL EAIAPEYGLT QLTYRGFERS LGFRTAPLGA ADVVEGVSAL
     LIGAHGIKIE VDTPGMVFAS SSTTPLGNSF GANLNPANPN AQTSELFATK RVWNLGTALT
     DSITSAIGAA TGNAWSNDQT EANGGLHVDV SSTQPALAAV ASVQDVGALE RAERRVATSN
     FYEAYRALDF SRSTSVDLLR SSVLLSEALH RKIVARGISL ITNQSIRTLK NFRLAILRDG
     ADLELFTHVD VLARLADWLT TSLRDIIVQQ QASTNAQRKK QPAASTRLPF ILAALHRAKD
     TFLVLGSTAS AIDGVVQHNH FTAAFQHAAS VSRARVRHDH FRTCSIHVRT SDLAAFVENV
     HLKIK