ID   TSDB_PSEU5              Reviewed;         216 AA.
AC   A4VND9;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Thiosulfate dehydrogenase electron acceptor;
DE   Flags: Precursor;
GN   Name=tsdB; OrderedLocusNames=PST_2844;
OS   Pseudomonas stutzeri (strain A1501).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=379731;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=A1501;
RX   PubMed=18495935; DOI=10.1073/pnas.0801093105;
RA   Yan Y., Yang J., Dou Y., Chen M., Ping S., Peng J., Lu W., Zhang W.,
RA   Yao Z., Li H., Liu W., He S., Geng L., Zhang X., Yang F., Yu H., Zhan Y.,
RA   Li D., Lin Z., Wang Y., Elmerich C., Lin M., Jin Q.;
RT   "Nitrogen fixation island and rhizosphere competence traits in the genome
RT   of root-associated Pseudomonas stutzeri A1501.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:7564-7569(2008).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=22779704; DOI=10.1111/j.1462-2920.2012.02820.x;
RA   Denkmann K., Grein F., Zigann R., Siemen A., Bergmann J., van Helmont S.,
RA   Nicolai A., Pereira I.A., Dahl C.;
RT   "Thiosulfate dehydrogenase: a widespread unusual acidophilic c-type
RT   cytochrome.";
RL   Environ. Microbiol. 14:2673-2688(2012).
CC   -!- FUNCTION: Acts as an electron acceptor for the thiosulfate
CC       dehydrogenase TsdA. {ECO:0000250}.
CC   -!- PTM: Binds 2 heme c groups covalently per subunit. {ECO:0000250}.
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DR   EMBL; CP000304; ABP80490.1; -; Genomic_DNA.
DR   RefSeq; WP_011913947.1; NC_009434.1.
DR   AlphaFoldDB; A4VND9; -.
DR   SMR; A4VND9; -.
DR   STRING; 379731.PST_2844; -.
DR   EnsemblBacteria; ABP80490; ABP80490; PST_2844.
DR   KEGG; psa:PST_2844; -.
DR   eggNOG; COG2863; Bacteria.
DR   HOGENOM; CLU_076280_0_1_6; -.
DR   OMA; PECVTCH; -.
DR   Proteomes; UP000000233; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:InterPro.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   Gene3D; 1.10.760.10; -; 2.
DR   InterPro; IPR009056; Cyt_c-like_dom.
DR   InterPro; IPR036909; Cyt_c-like_dom_sf.
DR   InterPro; IPR024167; Cytochrome_c4-like.
DR   Pfam; PF00034; Cytochrom_C; 2.
DR   PIRSF; PIRSF000005; Cytochrome_c4; 1.
DR   SUPFAM; SSF46626; SSF46626; 2.
DR   PROSITE; PS51007; CYTC; 2.
PE   3: Inferred from homology;
KW   Heme; Iron; Metal-binding; Reference proteome; Repeat; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..216
FT                   /note="Thiosulfate dehydrogenase electron acceptor"
FT                   /id="PRO_0000430265"
FT   DOMAIN          23..108
FT                   /note="Cytochrome c 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
FT   DOMAIN          118..210
FT                   /note="Cytochrome c 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
FT   BINDING         41
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /note="covalent"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
FT   BINDING         44
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /note="covalent"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
FT   BINDING         45
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="1"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
FT   BINDING         141
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /note="covalent"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
FT   BINDING         144
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /note="covalent"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
FT   BINDING         145
FT                   /ligand="heme c"
FT                   /ligand_id="ChEBI:CHEBI:61717"
FT                   /ligand_label="2"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00433"
SQ   SEQUENCE   216 AA;  22328 MW;  B484EE1860C2F3B1 CRC64;
     MKSIHWPLAG VAALLLSMQA QAADGQKIYT QGGANPAAMA CATCHGADAM GMAAAGFPRL
     AGIDAGYSRK QLDDFRSGAR SNPIMQPIAA ALSDEEMDAV AATLEAMPAP DFAAIGRSEA
     AEGVGARLAL RGAWERNIPE CVACHGPAGM GVGASFPPLA GQSAQYLGAQ LNAWRQGTRK
     NDPNDLMGHI ARAMSDDEVQ AVAEYFANVG QKETGQ