ID   TSE4_PSEAE              Reviewed;         195 AA.
AC   Q9I069;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Toxin protein Tse4 {ECO:0000303|PubMed:24589350};
GN   Name=tse4 {ECO:0000303|PubMed:24589350}; OrderedLocusNames=PA2774;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=24589350; DOI=10.1111/mmi.12571;
RA   Whitney J.C., Beck C.M., Goo Y.A., Russell A.B., Harding B.N.,
RA   De Leon J.A., Cunningham D.A., Tran B.Q., Low D.A., Goodlett D.R.,
RA   Hayes C.S., Mougous J.D.;
RT   "Genetically distinct pathways guide effector export through the type VI
RT   secretion system.";
RL   Mol. Microbiol. 92:529-542(2014).
CC   -!- FUNCTION: Toxin secreted by the H1 type VI (H1-T6SS) secretion system
CC       into the periplasm of recipient cells. {ECO:0000269|PubMed:24589350}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000269|PubMed:24589350}.
CC       Secreted {ECO:0000269|PubMed:24589350}. Note=Delivered to the target
CC       cell periplasm by the H1 type VI (H1-T6SS) secretion system.
CC       {ECO:0000269|PubMed:24589350}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant together with deletion of its
CC       cognate immunity protein Tsi4 leads to a significant loss of fitness
CC       advantage when placed in competition with parental strains.
CC       {ECO:0000269|PubMed:24589350}.
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DR   EMBL; AE004091; AAG06162.1; -; Genomic_DNA.
DR   PIR; E83298; E83298.
DR   RefSeq; NP_251464.1; NC_002516.2.
DR   RefSeq; WP_003099160.1; NZ_QZGE01000011.1.
DR   AlphaFoldDB; Q9I069; -.
DR   STRING; 287.DR97_5174; -.
DR   PaxDb; Q9I069; -.
DR   PRIDE; Q9I069; -.
DR   EnsemblBacteria; AAG06162; AAG06162; PA2774.
DR   GeneID; 882745; -.
DR   KEGG; pae:PA2774; -.
DR   PATRIC; fig|208964.12.peg.2912; -.
DR   PseudoCAP; PA2774; -.
DR   HOGENOM; CLU_120465_0_0_6; -.
DR   OMA; DITGPCM; -.
DR   BioCyc; PAER208964:G1FZ6-2821-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   4: Predicted;
KW   Host membrane; Membrane; Reference proteome; Secreted; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   CHAIN           1..195
FT                   /note="Toxin protein Tse4"
FT                   /id="PRO_0000449106"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        171..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   195 AA;  19214 MW;  A4BAC5447C1CC09F CRC64;
     MSQLPIPLPL NESRWRYVTA SGGGMTVAFL AGSGGSITLL SPEGENVSFR YGGVGGGIGL
     GMRLPRFGKV NLNVKGKSVG GAGALEALPS TGTVLVADGL VGRDLSRGDF TGPCMYVELG
     AGVIAGGSGT AILFGLDPKL LAAVALASAS PLTAALGASM SRQLLQSSRG ALLMAGVNVG
     AQFGGGAAAY LGALF