ID   TSE5_PSEAE              Reviewed;        1317 AA.
AC   Q9I0F4;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Toxin protein Tse5 {ECO:0000303|PubMed:24794869};
GN   Name=tse5 {ECO:0000303|PubMed:24794869}; OrderedLocusNames=PA2684;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION.
RC   STRAIN=PAK;
RX   PubMed=24794869; DOI=10.1074/jbc.m114.563429;
RA   Hachani A., Allsopp L.P., Oduko Y., Filloux A.;
RT   "The VgrG proteins are 'a la carte' delivery systems for bacterial type VI
RT   effectors.";
RL   J. Biol. Chem. 289:17872-17884(2014).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=24589350; DOI=10.1111/mmi.12571;
RA   Whitney J.C., Beck C.M., Goo Y.A., Russell A.B., Harding B.N.,
RA   De Leon J.A., Cunningham D.A., Tran B.Q., Low D.A., Goodlett D.R.,
RA   Hayes C.S., Mougous J.D.;
RT   "Genetically distinct pathways guide effector export through the type VI
RT   secretion system.";
RL   Mol. Microbiol. 92:529-542(2014).
CC   -!- FUNCTION: Toxin secreted by the H1 type VI (H1-T6SS) secretion system
CC       that acts on bacterial target cells. The producing bacterium is
CC       protected by a cognate immunity protein. {ECO:0000269|PubMed:24589350,
CC       ECO:0000269|PubMed:24794869}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant together with deletion of its
CC       cognate immunity protein Tsi5 leads to a significant loss of fitness
CC       advantage when placed in competition with parental strains.
CC       {ECO:0000269|PubMed:24589350}.
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DR   EMBL; AE004091; AAG06072.1; -; Genomic_DNA.
DR   PIR; F83310; F83310.
DR   RefSeq; NP_251374.1; NC_002516.2.
DR   RefSeq; WP_003115323.1; NZ_QZGE01000008.1.
DR   AlphaFoldDB; Q9I0F4; -.
DR   SMR; Q9I0F4; -.
DR   STRING; 287.DR97_5275; -.
DR   PaxDb; Q9I0F4; -.
DR   PRIDE; Q9I0F4; -.
DR   EnsemblBacteria; AAG06072; AAG06072; PA2684.
DR   GeneID; 879129; -.
DR   KEGG; pae:PA2684; -.
DR   PATRIC; fig|208964.12.peg.2808; -.
DR   PseudoCAP; PA2684; -.
DR   HOGENOM; CLU_001218_0_1_6; -.
DR   InParanoid; Q9I0F4; -.
DR   OMA; CEDIANS; -.
DR   PhylomeDB; Q9I0F4; -.
DR   BioCyc; PAER208964:G1FZ6-2724-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0097351; F:toxin sequestering activity; IMP:PseudoCAP.
DR   GO; GO:0033103; P:protein secretion by the type VI secretion system; IDA:PseudoCAP.
DR   InterPro; IPR045351; DUF6531.
DR   InterPro; IPR001826; RHS.
DR   InterPro; IPR022385; Rhs_assc_core.
DR   InterPro; IPR031325; RHS_repeat.
DR   InterPro; IPR006530; YD.
DR   Pfam; PF20148; DUF6531; 1.
DR   Pfam; PF03527; RHS; 1.
DR   Pfam; PF05593; RHS_repeat; 5.
DR   PRINTS; PR00394; RHSPROTEIN.
DR   TIGRFAMs; TIGR03696; Rhs_assc_core; 1.
DR   TIGRFAMs; TIGR01643; YD_repeat_2x; 6.
PE   4: Predicted;
KW   Reference proteome; Virulence.
FT   CHAIN           1..1317
FT                   /note="Toxin protein Tse5"
FT                   /id="PRO_0000449107"
FT   REGION          395..419
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1317 AA;  145821 MW;  89A15B0FCBA05518 CRC64;
     MSGLPVSHVG EKVSGGVIST GSPTVHVGSS AVGLADRVSA CVPLVGKPVN PMLGSKLLPE
     EVDFALAAPD TFTFARGYLS SNPRIGRLGR GWWLPGESMH LELSEDACVL VDAQGRRIGF
     PALAPGAQHY SGSEELWLRR GGSSGGEAQA WRGRWAAVPA ELQTQEGSVL VLSGHSYLHF
     QRCPDGIWRL QASFGRAGYR TEFRWSGRGL LTGVRDSAGR SYALVYQQAC EPSEGDDGLR
     LFGVILASHD GPPPDYIDPQ SPGLDWLVRY QFSDSGDLIA VRDRLGQVVR VFAWREHMLV
     AHGEPGGLEV RYEWDVHAPH GRVVKQIEAG GLTRTFRYLR DATEVSDSLG RVERYEFAGE
     GGQRRWTALV RADGSRSEFD YDLFGRLVAM RDPLGRETRR RRDGQGRMLE EESPGKARYR
     KRVDEETGLL VELEDAMQRR WTFERDERGN ATTVRGPAGS TRYAYEDPRL PDRPTRIVDP
     RGGERRLEWN RFGLLAALTD CSGQVWRYDY DNEGRLVASS DPLGQLTRRR YDPLGQLIGL
     ELADGSALSY EYDALGRQTR IADAEGHATL FSWGHGDLLA RVSDAGGGEL SYLHDEAGRL
     VALTNENGVQ AQFRYDLLDR LVEETGFDGR RQRYRYNAAD ELIAREDADG RETTYAYDRD
     GRLASIRVPA TEHAPALVER YRWLADGRLA SAGGADCEVR YTYDEVGNLR LESQVHADGW
     VYSVEHSHDA LGVRQTSRYG DAPPVAWLTY GPGHLHGALV GAVELAFERD ALHREVRRDA
     RRDGQDDALF TQERQHAPLG RLQRSRLRLA GGFDWQRGYR YDGLGQLVGI DDNQYPSVRY
     EYDLGGRLLA SRRAGAAAST YRYDAAGNRL EGVGEHARED ARQAFAENEL YRSGFSRSET
     RASQAGEGPA RWAGNRVERI AGNRYRFDAL GNLVERIGAD GERLRLAYDG AQRLVHLTRD
     YADGTRLEAR YRYDALSRRI AKVVLRDGVE QQVRFGWDGD RQCAEAFARE LRTTVHEPGG
     FVPLLRLEQA CEPDPPELLQ LRQAFAAEGQ PLPAQCVPAL GEARIAFFHT DHLGTPLQLS
     DERGQLRWQG VPDDWRAVAP ERQPGAQPIR FQGQYHDEES GLYYNRYRYY LPEAGRYASQ
     DPLGLGGGPN PYAYALNAPT LAYDPTGLII PLVVIGAFAA RAAIGAALGA GIELGMQTGK
     QVLGQMKDNW DSDRDLTDIK WKCIDINWKH VGASAAIGTV APGMLSTGKT VVQSAKAIRT
     LSGQAANTAN RAAKLAARKA AHADTIKKAV ATQAAWQTGK QIVKCPLKDE EEECPPQ