ID   TSE7_PSEAE              Reviewed;         386 AA.
AC   Q9I733;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=DNase toxin Tse7 {ECO:0000303|PubMed:30455305};
DE            EC=3.1.21.1 {ECO:0000269|PubMed:30455305};
GN   Name=tse7 {ECO:0000303|PubMed:30455305}; OrderedLocusNames=PA0099;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, INTERACTION WITH TSI7, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=30455305; DOI=10.1073/pnas.1814181115;
RA   Pissaridou P., Allsopp L.P., Wettstadt S., Howard S.A., Mavridou D.A.I.,
RA   Filloux A.;
RT   "The Pseudomonas aeruginosa T6SS-VgrG1b spike is topped by a PAAR protein
RT   eliciting DNA damage to bacterial competitors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:12519-12524(2018).
CC   -!- FUNCTION: Type VI secretion exported toxin that via to its DNase
CC       activity induces growth arrest and ultimately DNA degradation within
CC       target cell (PubMed:30455305). The activity is initially neutralized by
CC       a cognate immunity protein Tsi7 (PubMed:30455305).
CC       {ECO:0000269|PubMed:30455305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-
CC         phosphooligonucleotide end-products.; EC=3.1.21.1;
CC         Evidence={ECO:0000269|PubMed:30455305};
CC   -!- SUBUNIT: Interacts with Tsi7. {ECO:0000269|PubMed:30455305}.
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DR   EMBL; AE004091; AAG03489.1; -; Genomic_DNA.
DR   PIR; H83633; H83633.
DR   RefSeq; NP_248789.1; NC_002516.2.
DR   RefSeq; WP_003115783.1; NZ_QZGE01000015.1.
DR   AlphaFoldDB; Q9I733; -.
DR   STRING; 287.DR97_3056; -.
DR   PaxDb; Q9I733; -.
DR   PRIDE; Q9I733; -.
DR   EnsemblBacteria; AAG03489; AAG03489; PA0099.
DR   GeneID; 880737; -.
DR   KEGG; pae:PA0099; -.
DR   PATRIC; fig|208964.12.peg.103; -.
DR   PseudoCAP; PA0099; -.
DR   HOGENOM; CLU_066858_1_0_6; -.
DR   OMA; VYANNME; -.
DR   BioCyc; PAER208964:G1FZ6-101-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0004530; F:deoxyribonuclease I activity; IEA:UniProtKB-EC.
DR   GO; GO:0033103; P:protein secretion by the type VI secretion system; IMP:PseudoCAP.
DR   InterPro; IPR028917; Tox-GHH2_domain.
DR   InterPro; IPR025425; Tox_PAAR-like.
DR   Pfam; PF13665; DUF4150; 1.
DR   Pfam; PF15635; Tox-GHH2; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome; Virulence.
FT   CHAIN           1..386
FT                   /note="DNase toxin Tse7"
FT                   /id="PRO_0000449109"
SQ   SEQUENCE   386 AA;  41321 MW;  18E7480CDCB15BF9 CRC64;
     MANEVYANNM EISCKAANGK SIAAFPDVCF TPPQAPPTPL GVPIPYPNTG LSKDTTKGTR
     TIRITRKEVM LKNKSYYKTS YGDEPGRAPK KGIVTSKIKG KVYFTSWSMN VKFESKNVVR
     HLDLTTHNHA SFPGNTPVWP YLDQATVDAG GGPCSNEVKK EKKDCADFKP HGSKDACAGL
     GAGKPSGKKT SNEADRLADK VAARKCLTAR RCALQPYKPN SCCPQQTAHH LIEASALHDK
     GRGGKGSVPL KGISNYSENK APCVCAEGVN QNVGTHGLMH TFQSAAAAKS RSGTLQLSNG
     SSISAKKTTY GTAKRQSMAA MGKVFPQSKC SKECLSAQLD NYHKQCGINA RTPIKAVETG
     QTDVTAATQA IKTRNARLGA TRSRVR