ID   TSEL_VIBCH              Reviewed;         641 AA.
AC   Q9KS43;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Toxin TseL {ECO:0000303|PubMed:23362380};
GN   Name=tseL {ECO:0000303|PubMed:23362380}; OrderedLocusNames=VC_1418;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [2]
RP   FUNCTION, INTERACTION WITH VGRG3, SUBCELLULAR LOCATION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=V52;
RX   PubMed=23362380; DOI=10.1073/pnas.1222783110;
RA   Dong T.G., Ho B.T., Yoder-Himes D.R., Mekalanos J.J.;
RT   "Identification of T6SS-dependent effector and immunity proteins by Tn-seq
RT   in Vibrio cholerae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:2623-2628(2013).
RN   [3]
RP   FUNCTION.
RX   PubMed=24348240; DOI=10.1371/journal.ppat.1003752;
RA   Miyata S.T., Unterweger D., Rudko S.P., Pukatzki S.;
RT   "Dual expression profile of type VI secretion system immunity genes
RT   protects pandemic Vibrio cholerae.";
RL   PLoS Pathog. 9:E1003752-E1003752(2013).
CC   -!- FUNCTION: Toxin secreted by the type VI (T6SS) secretion system that
CC       acts on prokaryotic as well as eukaryotic target cells.
CC       {ECO:0000269|PubMed:23362380, ECO:0000269|PubMed:24348240}.
CC   -!- SUBUNIT: Interacts with VgrG3; this interaction allows TseL secretion
CC       to target cells. {ECO:0000269|PubMed:23362380}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23362380}.
CC       Note=Delivered to the target cell by the type VI (T6SS) secretion
CC       system in a VgrG3-dependent pathway. {ECO:0000269|PubMed:23362380}.
CC   -!- DISRUPTION PHENOTYPE: Deletion does not abolish killing activity.
CC       However, triple mutant lacking tseL, vasX, and vgrG3 fails to kill
CC       Escherichia coli. {ECO:0000269|PubMed:23362380}.
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DR   EMBL; AE003852; AAF94575.1; -; Genomic_DNA.
DR   PIR; F82202; F82202.
DR   RefSeq; NP_231061.1; NC_002505.1.
DR   RefSeq; WP_000376836.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9KS43; -.
DR   STRING; 243277.VC_1418; -.
DR   ESTHER; vibch-VC1418; Lipase_3.
DR   TCDB; 1.C.132.1.1; the tsel toxin (tsel) family.
DR   PRIDE; Q9KS43; -.
DR   DNASU; 2614050; -.
DR   EnsemblBacteria; AAF94575; AAF94575; VC_1418.
DR   GeneID; 57740070; -.
DR   KEGG; vch:VC_1418; -.
DR   PATRIC; fig|243277.26.peg.1347; -.
DR   eggNOG; COG3675; Bacteria.
DR   HOGENOM; CLU_033066_0_0_6; -.
DR   OMA; FLEAYQC; -.
DR   BioCyc; VCHO:VC1418-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002921; Fungal_lipase-like.
DR   Pfam; PF01764; Lipase_3; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Secreted; Virulence.
FT   CHAIN           1..641
FT                   /note="Toxin TseL"
FT                   /id="PRO_0000449206"
SQ   SEQUENCE   641 AA;  72238 MW;  DF8C92B5648077D1 CRC64;
     MDSFNYCVQC NPEENWLELE FRSENDEPID GLLVTITNQS APSNTYTQTT SSGKVLFGKI
     AAGEWRASVS QASLLTEVEK YASRKEGQES PVKKRAAAEL DAADKDTKQY RFTTIGDFWD
     EAPKDEFLQK QHKGIDVNAS AEKAGFRLSH NQTYVFEIKA LRSYMPVIID TDEFNLVNSY
     TFALLSKLAY ATNDFNRDDG KTIDNQGAIS TVISQLKRKE RPTYSGDLQA KWLLEEIPYS
     KALSAQYYAE DDVGSEGYII FNDELAIIGV RGTEPYFQSK KPPVDNTKFK IIKAASGMAA
     VIADKIESAT DSPGMKDLII TDLDAAQIAP EEFGGTYVHR GFYQYTMALL SLMEKDLGLH
     KIKKFYCCGH SLGGAGALLI SALIKDSYHP PVLRLYTYGM PRVGTRSFVE RYQNILHYRH
     VNNHDLVPQI PTVWMNTDVS EGFHVLDVFK SRVDLMRKML TDDDDDNYQH HGHLSQLLTY
     NSNNQVLLTP KQTQVTMLDL ANLATNDSVA MVDGLSDASI VEHGMEQYIP NLFEQLTALS
     DESLMVHYQR AISALEQEIA TLQQSYLTVK QAWIESIGNG TPTMNIGRLM SEMHSINKLI
     ENRNKIRGEL RQIVSDPQRM PATKFLISQQ TLPDEIKVQI R