TSG10_MOUSE
ID TSG10_MOUSE Reviewed; 697 AA.
AC Q6NY15; Q68RJ4; Q8K3V2;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Testis-specific gene 10 protein;
GN Name=Tsga10;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, PROTEOLYTIC
RP PROCESSING, AND FUNCTION.
RX PubMed=14585816; DOI=10.1095/biolreprod.103.021170;
RA Modarressi M.H., Behnam B., Cheng M., Taylor K.E., Wolfe J.,
RA van der Hoorn F.A.;
RT "Tsga10 encodes a 65-kilodalton protein that is processed to the 27-
RT kilodalton fibrous sheath protein.";
RL Biol. Reprod. 70:608-615(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16643851; DOI=10.1016/j.bbrc.2006.03.240;
RA Behnam B., Modarressi M.H., Conti V., Taylor K.E., Puliti A., Wolfe J.;
RT "Expression of Tsga10 sperm tail protein in embryogenesis and neural
RT development: from cilium to cell division.";
RL Biochem. Biophys. Res. Commun. 344:1102-1110(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 562-575, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [5]
RP FUNCTION, INTERACTION WITH HIF1A, AND SUBCELLULAR LOCATION.
RX PubMed=16777103; DOI=10.1016/j.febslet.2006.05.058;
RA Haegele S., Behnam B., Borter E., Wolfe J., Paasch U., Lukashev D.,
RA Sitkovsky M., Wenger R.H., Katschinski D.M.;
RT "TSGA10 prevents nuclear localization of the hypoxia-inducible factor
RT (HIF)-1alpha.";
RL FEBS Lett. 580:3731-3738(2006).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a role in spermatogenesis (PubMed:14585816). When
CC overexpressed, prevents nuclear localization of HIF1A
CC (PubMed:16777103). {ECO:0000269|PubMed:14585816,
CC ECO:0000269|PubMed:16777103}.
CC -!- SUBUNIT: Interacts with HIF1A. {ECO:0000269|PubMed:16777103}.
CC -!- INTERACTION:
CC Q6NY15; Q61221-1: Hif1a; NbExp=2; IntAct=EBI-8549230, EBI-8549331;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9Z220}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,
CC centriole {ECO:0000250|UniProtKB:Q9BZW7}. Note=In mature spermatozoa,
CC localizes to the centriole and midpiece (By similarity). The 27-kDa
CC peptide associates with the fibrous sheath in mature spermatozoa and
CC localizes to the principal piece of sperm tail, while the 55-kDa
CC peptide localizes to the midpiece (PubMed:16643851, PubMed:16777103).
CC Detected in the cytoplasm of almost all spermatogonial cells within the
CC seminiferous tubules (By similarity). {ECO:0000250|UniProtKB:Q9BZW7,
CC ECO:0000250|UniProtKB:Q9Z220, ECO:0000269|PubMed:16643851,
CC ECO:0000269|PubMed:16777103}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6NY15-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6NY15-2; Sequence=VSP_028580;
CC Name=3;
CC IsoId=Q6NY15-3; Sequence=VSP_028579, VSP_028580;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in testis, in spermatozoa
CC (at protein level) (PubMed:14585816, PubMed:16643851). Not detected in
CC Leydig cells (PubMed:16643851). The N-terminal 27-kDa fragment is also
CC detected in liver, while the C-terminal 55-kDa fragment is also found
CC retina, brain and kidney (at protein level) (PubMed:16643851).
CC {ECO:0000269|PubMed:14585816, ECO:0000269|PubMed:16643851}.
CC -!- DEVELOPMENTAL STAGE: First detected at 7.5 dpc (PubMed:16643851). Tends
CC to be expressed weakly during the early stages of embryogenesis.
CC Expression increases over time in different growing and differentiating
CC tissues, including brain (11.5 dpc), vertebrae (14.5 dpc), primordia of
CC vibrissae follicle (15.5 dpc), intestinal sub-endothelium (16.5 dpc),
CC pancreas (16.5 dpc), eyeball (16.5 dpc), liver (16.5 dpc),
CC telencephalon (18.5 dpc) (PubMed:16643851). In the developing neural
CC tube at 8.5-9.0 dpc, preferentially expressed in cells with one or more
CC neuronal extensions (PubMed:16643851). During spermatogenesis, weakly
CC detected in primary spermatocytes, but expression increases, reaching
CC strong levels in elongated spermatids (stage V) and mature sperm (at
CC protein level) (PubMed:16643851). {ECO:0000269|PubMed:16643851}.
CC -!- PTM: Processed into N-terminal 27-kDa and C-terminal 55-kDa fragments.
CC {ECO:0000269|PubMed:14585816}.
CC -!- SIMILARITY: Belongs to the CEP135/TSGA10 family. {ECO:0000305}.
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DR EMBL; AF530050; AAN01136.3; -; mRNA.
DR EMBL; AY618264; AAT68665.1; -; mRNA.
DR EMBL; BC066782; AAH66782.1; -; mRNA.
DR CCDS; CCDS14894.1; -. [Q6NY15-1]
DR CCDS; CCDS69883.1; -. [Q6NY15-3]
DR CCDS; CCDS78571.1; -. [Q6NY15-2]
DR RefSeq; NP_001277649.1; NM_001290720.1. [Q6NY15-3]
DR RefSeq; NP_001277650.1; NM_001290721.1. [Q6NY15-2]
DR RefSeq; NP_997111.1; NM_207228.3. [Q6NY15-1]
DR AlphaFoldDB; Q6NY15; -.
DR SMR; Q6NY15; -.
DR BioGRID; 229238; 2.
DR IntAct; Q6NY15; 2.
DR MINT; Q6NY15; -.
DR STRING; 10090.ENSMUSP00000048859; -.
DR iPTMnet; Q6NY15; -.
DR PhosphoSitePlus; Q6NY15; -.
DR MaxQB; Q6NY15; -.
DR PaxDb; Q6NY15; -.
DR PeptideAtlas; Q6NY15; -.
DR PRIDE; Q6NY15; -.
DR ProteomicsDB; 300043; -. [Q6NY15-1]
DR ProteomicsDB; 300044; -. [Q6NY15-2]
DR ProteomicsDB; 300045; -. [Q6NY15-3]
DR Antibodypedia; 32767; 141 antibodies from 20 providers.
DR DNASU; 211484; -.
DR Ensembl; ENSMUST00000041815; ENSMUSP00000048859; ENSMUSG00000060771. [Q6NY15-1]
DR Ensembl; ENSMUST00000088072; ENSMUSP00000085391; ENSMUSG00000060771. [Q6NY15-3]
DR Ensembl; ENSMUST00000114902; ENSMUSP00000110552; ENSMUSG00000060771. [Q6NY15-2]
DR GeneID; 211484; -.
DR KEGG; mmu:211484; -.
DR UCSC; uc007arz.2; mouse. [Q6NY15-1]
DR UCSC; uc007asb.1; mouse. [Q6NY15-3]
DR CTD; 80705; -.
DR MGI; MGI:2685063; Tsga10.
DR VEuPathDB; HostDB:ENSMUSG00000060771; -.
DR eggNOG; ENOG502RJHC; Eukaryota.
DR GeneTree; ENSGT00940000159205; -.
DR HOGENOM; CLU_008607_2_1_1; -.
DR InParanoid; Q6NY15; -.
DR OrthoDB; 139407at2759; -.
DR PhylomeDB; Q6NY15; -.
DR TreeFam; TF326518; -.
DR BioGRID-ORCS; 211484; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Tsga10; mouse.
DR PRO; PR:Q6NY15; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q6NY15; protein.
DR Bgee; ENSMUSG00000060771; Expressed in spermatid and 211 other tissues.
DR ExpressionAtlas; Q6NY15; baseline and differential.
DR GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0031514; C:motile cilium; IDA:MGI.
DR GO; GO:0043005; C:neuron projection; IDA:MGI.
DR GO; GO:0035686; C:sperm fibrous sheath; IDA:MGI.
DR GO; GO:0097228; C:sperm principal piece; IDA:MGI.
DR GO; GO:0005198; F:structural molecule activity; ISO:MGI.
DR GO; GO:0030031; P:cell projection assembly; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IEA:InterPro.
DR InterPro; IPR033323; TSGA10.
DR PANTHER; PTHR23159:SF14; PTHR23159:SF14; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Direct protein sequencing;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..697
FT /note="Testis-specific gene 10 protein"
FT /id="PRO_0000307128"
FT REGION 556..688
FT /note="Interaction with HIF1A"
FT /evidence="ECO:0000269|PubMed:16777103"
FT REGION 656..684
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 669..684
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 687
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Z220"
FT VAR_SEQ 469..538
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16643851"
FT /id="VSP_028579"
FT VAR_SEQ 691..697
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:14585816,
FT ECO:0000303|PubMed:16643851"
FT /id="VSP_028580"
FT CONFLICT 353..355
FT /note="END -> QNT (in Ref. 1; AAN01136 and 2; AAT68665)"
FT /evidence="ECO:0000305"
FT CONFLICT 647
FT /note="Missing (in Ref. 1; AAN01136 and 2; AAT68665)"
FT /evidence="ECO:0000305"
FT CONFLICT 660
FT /note="L -> F (in Ref. 1; AAN01136 and 2; AAT68665)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 697 AA; 81262 MW; 8B4200E1B4FD59C6 CRC64;
MMRNRSKSPR RPSPTSRAAN CDVELLKSTA RDREELKCML EKYERHLAEI QGNVKVLTSE
RDKTFLLYEQ AQEEIARLRR EMMKSCKSPK STTAHAILRR VETERDVAFT DLRRMTTERD
SLRERLKIAQ ETAFNEKAHL EQRIEELECT VHNLDDERME QMANMTLMKE TITTVEKEMK
SLARKAMDTE SELGRQKAEN NSLRLLYENT EKDLSDTQRH LAKKKYELQL TQEKIMCLDE
KIDNFTRQNI AQREEISILG ATLNDLAKEK ECLQACLDKK SENIASLGES LAMKEKTISG
MKNIIAEMEQ ASRQSTEALI MCEQDISRMR RQLDETNDEL GQIARERDIL AHENDNLQEQ
FAKVKQENQA LSKKLNDTHN ELSDIKQKVQ DTNLEVNKLK NILKSEESEN RQIMEQLRKA
NEDAENWENK ARQTEAENNT LKLELITAEA EGNRLKEKVD ALNREVEQHL NAERSYKSQI
ATLHKSLVKM EEELQKVQFE KVSALADLSS TRELCIKLDS SKELLNRQLV AKDQEIEMME
NELDSARSEI ELLRSQMTNE RISMQNLEAL LVANRDKEYQ SQIALQEKES EIQLLKEHLC
LAENKMAIQS RDVAQFRNVV TQLEADLDIT KRQLGTERFE RERAVQELRR QNYSSNAYNL
GPMKPNTKCH SPERAHHRSP DRGLDRSLEE NLCYRDF
