TSG10_RAT
ID TSG10_RAT Reviewed; 712 AA.
AC Q9Z220;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 2.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Testis-specific gene 10 protein;
DE AltName: Full=Cp431;
GN Name=Tsga10;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RA Petersen C., Hoyer-Fender S.;
RL Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=14585816; DOI=10.1095/biolreprod.103.021170;
RA Modarressi M.H., Behnam B., Cheng M., Taylor K.E., Wolfe J.,
RA van der Hoorn F.A.;
RT "Tsga10 encodes a 65-kilodalton protein that is processed to the 27-
RT kilodalton fibrous sheath protein.";
RL Biol. Reprod. 70:608-615(2004).
RN [3]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16643851; DOI=10.1016/j.bbrc.2006.03.240;
RA Behnam B., Modarressi M.H., Conti V., Taylor K.E., Puliti A., Wolfe J.;
RT "Expression of Tsga10 sperm tail protein in embryogenesis and neural
RT development: from cilium to cell division.";
RL Biochem. Biophys. Res. Commun. 344:1102-1110(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-702, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Plays a role in spermatogenesis (By similarity). When
CC overexpressed, prevents nuclear localization of HIF1A (By similarity).
CC {ECO:0000250|UniProtKB:Q6NY15, ECO:0000250|UniProtKB:Q9BZW7}.
CC -!- SUBUNIT: Interacts with HIF1A. {ECO:0000250|UniProtKB:Q6NY15}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14585816,
CC ECO:0000269|PubMed:16643851}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome, centriole
CC {ECO:0000250|UniProtKB:Q9BZW7}. Note=In mature spermatozoa, localizes
CC to the centriole and midpiece (By similarity). The 27-kDa N-terminal
CC fragment associates with the fibrous sheath in mature spermatozoa and
CC localizes to the principal piece of sperm tail, while the 55-kDa
CC fragment localizes to the midpiece (PubMed:14585816). Detected in the
CC cytoplasm of almost all spermatogonial cells within the seminiferous
CC tubules (PubMed:16643851). {ECO:0000250|UniProtKB:Q9BZW7,
CC ECO:0000269|PubMed:14585816, ECO:0000269|PubMed:16643851}.
CC -!- TISSUE SPECIFICITY: Expressed in testis, predominantly in elongated
CC spermatids (at protein level) (PubMed:14585816, PubMed:16643851).
CC Detected in spermatocytes only at the mRNA, but not at the protein
CC level (PubMed:14585816). {ECO:0000269|PubMed:14585816,
CC ECO:0000269|PubMed:16643851}.
CC -!- DEVELOPMENTAL STAGE: Becomes detectable in testis between postnatal
CC days 15 and 21 and expression levels remain high in the adult. Not
CC detected in fetal testis. {ECO:0000269|PubMed:14585816}.
CC -!- PTM: Processed into N-terminal 27-kDa and C-terminal 55-kDa fragments.
CC {ECO:0000269|PubMed:14585816}.
CC -!- SIMILARITY: Belongs to the CEP135/TSGA10 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC72234.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF092091; AAC72234.1; ALT_INIT; mRNA.
DR RefSeq; NP_001025193.1; NM_001030022.1.
DR AlphaFoldDB; Q9Z220; -.
DR SMR; Q9Z220; -.
DR iPTMnet; Q9Z220; -.
DR PhosphoSitePlus; Q9Z220; -.
DR PaxDb; Q9Z220; -.
DR GeneID; 252923; -.
DR KEGG; rno:252923; -.
DR UCSC; RGD:621142; rat.
DR CTD; 80705; -.
DR RGD; 621142; Tsga10.
DR eggNOG; ENOG502RJHC; Eukaryota.
DR InParanoid; Q9Z220; -.
DR OrthoDB; 139407at2759; -.
DR PhylomeDB; Q9Z220; -.
DR PRO; PR:Q9Z220; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0031514; C:motile cilium; ISO:RGD.
DR GO; GO:0043005; C:neuron projection; ISO:RGD.
DR GO; GO:0035686; C:sperm fibrous sheath; ISO:RGD.
DR GO; GO:0097228; C:sperm principal piece; ISO:RGD.
DR GO; GO:0005198; F:structural molecule activity; IDA:MGI.
DR GO; GO:0030031; P:cell projection assembly; ISO:RGD.
DR GO; GO:0007283; P:spermatogenesis; IEA:InterPro.
DR InterPro; IPR033323; TSGA10.
DR PANTHER; PTHR23159:SF14; PTHR23159:SF14; 2.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome.
FT CHAIN 1..712
FT /note="Testis-specific gene 10 protein"
FT /id="PRO_0000307127"
FT REGION 571..703
FT /note="Interaction with HIF1A"
FT /evidence="ECO:0000250"
FT REGION 672..699
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 684..699
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 161
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BZW7"
FT MOD_RES 702
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 712 AA; 83012 MW; 21C96A82AABB9365 CRC64;
MMRNRSKSPR RPSPTSRAAN CDVDLLKSTA RDREELKCML EKYERHLAEI QGNVKVLTSV
RDKTFLLYEQ AQEEIARLRR EMMKSCQSPK STTAHAILRR VETERDVAFT DLRRMTTARD
SLRERLKIAQ AFNEKAHLEQ RIEELECTVH NLDDERMEQM SNMTLMKETI TIVEKEMKSL
ARKAMDTESE LGRQKAENNS LRLLYENTEK DLSDTQRHLA KKKYELQLTQ EKIMCLDEKI
DNFTRQNIAQ REEISILGAT LNDLAKEKEC LQTCLDKKSE NIASLGESLA MKEKTISGMK
NIIAEMEQAS RQSTEALIMC EQDISRMRRQ LDETNDELGQ IARERDILAH ENDNLQEQFA
KVKQENQALS KKLNDTHNEL SDIKQKVQDT NLEVNKLKNI LKSEESTNLE VNKLKNILKS
EESENRQIME QLRKANEDAE NWENKARQLE AENNTLKLEL ITAEAEGNRL KEKVDALNRE
VEQHLNAERS YKSQIATLHK SLVKMEEELQ KVQFEKVSAL ADLSSTRELC IKLDSSKELL
NRQLVAKDQE IEMMENELDS ARSEIELLRS QMTNERISMQ NLEALLVANR DKEYQSQIAL
QEKESEIQLL KEHLCLAENK MAIQSRDVAQ FRNVVTQLEA DLDITKRQLG TERFERERAV
QELRRQNYSS NAYHLGSMKP NTKCHSPERA HHRSPDRDLD RSLEENLCYR DF
