TSGA8_MOUSE
ID TSGA8_MOUSE Reviewed; 238 AA.
AC Q9JJL0; C4IXT6;
DT 10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Testis-specific gene A8 protein {ECO:0000312|MGI:MGI:1194903};
DE AltName: Full=Haploid-specific alanine-rich acidic protein located on chromosome X {ECO:0000303|PubMed:10993819};
DE Short=Halap-x {ECO:0000303|PubMed:10993819};
GN Name=Tsga8 {ECO:0000312|MGI:MGI:1194903};
GN Synonyms=Halapx {ECO:0000303|PubMed:10993819, ECO:0000312|MGI:MGI:1194903};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|EMBL:BAA96418.1};
RN [1] {ECO:0000312|EMBL:BAA96418.1}
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAA96418.1};
RC TISSUE=Testis {ECO:0000312|EMBL:BAA96418.1};
RX PubMed=10993819; DOI=10.1095/biolreprod63.4.993;
RA Uchida K., Tsuchida J., Tanaka H., Koga M., Nishina Y., Nozaki M.,
RA Yoshinaga K., Toshimori K., Matsumiya K., Okuyama A., Nishimune Y.;
RT "Cloning and characterization of a complementary deoxyribonucleic acid
RT encoding haploid-specific alanine-rich acidic protein located on
RT chromosome-X.";
RL Biol. Reprod. 63:993-999(2000).
RN [2] {ECO:0000312|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3] {ECO:0000312|EMBL:AAI39063.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis {ECO:0000312|EMBL:AAI39063.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10993819}. Nucleus,
CC nucleoplasm {ECO:0000269|PubMed:10993819}. Note=Strongly expressed in
CC the nucleus of round spermatids. As spermatids mature, nuclear
CC expression declines and cytoplasmic expression increases. Elongating
CC spermatids have mainly cytoplasmic expression.
CC {ECO:0000269|PubMed:10993819}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in testis (at protein
CC level). {ECO:0000269|PubMed:10993819}.
CC -!- DEVELOPMENTAL STAGE: Detected in testis from 18 days onwards. In
CC developing spermatazoa, expressed from the round spermatid stage
CC through to the terminal stages of spermiogenesis, when expression
CC declines. {ECO:0000269|PubMed:10993819}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI39063.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB032764; BAA96418.1; -; mRNA.
DR EMBL; CR382328; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC139062; AAI39063.1; ALT_INIT; mRNA.
DR CCDS; CCDS53124.1; -.
DR RefSeq; NP_068698.1; NM_021898.2.
DR AlphaFoldDB; Q9JJL0; -.
DR STRING; 10090.ENSMUSP00000043015; -.
DR PaxDb; Q9JJL0; -.
DR PRIDE; Q9JJL0; -.
DR ProteomicsDB; 297660; -.
DR Ensembl; ENSMUST00000049420; ENSMUSP00000043015; ENSMUSG00000035522.
DR GeneID; 100502723; -.
DR KEGG; mmu:100502723; -.
DR UCSC; uc009trk.2; mouse.
DR CTD; 100502723; -.
DR MGI; MGI:1194903; Tsga8.
DR VEuPathDB; HostDB:ENSMUSG00000035522; -.
DR GeneTree; ENSGT00720000109445; -.
DR HOGENOM; CLU_1165508_0_0_1; -.
DR InParanoid; Q9JJL0; -.
DR OMA; ILCGKGA; -.
DR OrthoDB; 1633229at2759; -.
DR BioGRID-ORCS; 100502723; 2 hits in 69 CRISPR screens.
DR PRO; PR:Q9JJL0; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q9JJL0; protein.
DR Bgee; ENSMUSG00000035522; Expressed in seminiferous tubule of testis and 10 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Repeat.
FT CHAIN 1..238
FT /note="Testis-specific gene A8 protein"
FT /id="PRO_0000439814"
FT REPEAT 79..93
FT /note="1-1"
FT REPEAT 94..108
FT /note="1-2"
FT REPEAT 109..123
FT /note="1-3"
FT REPEAT 124..138
FT /note="1-4"
FT REPEAT 153..158
FT /note="2-1"
FT REPEAT 171..176
FT /note="2-2"
FT REPEAT 180..185
FT /note="2-3"
FT REPEAT 189..194
FT /note="2-4"
FT REGION 35..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 79..148
FT /note="4 X 15 AA tandem repeats of A-A-A-A-A-P-E-A-A-A-S-
FT [PL]-E-S-S"
FT /evidence="ECO:0000305"
FT REGION 153..194
FT /note="4 X 6 AA repeats of P-A-A-P-E-A"
FT /evidence="ECO:0000305"
SQ SEQUENCE 238 AA; 22769 MW; FF368150FCB23723 CRC64;
MDVPTSSTDS QEVKPIMPIR TKAACKFYPP HILCGKGAKT NKRGKRGGAQ KASTKKDIGE
TTPPVAKKSK VAKEPTMLAA AAAPEAAASP ESSAAAAAPE AAASPESSAA AAAPEAAASP
ESSAAAAAPE AAASLESSAA AAAPEAAAAP ATPAAPEAAA APEVAAAPAT PAAPEATAAP
AAPEAATTPA APEAPAAAPA VEEEEMVWEA AAVVGEAAVK PPEEEPTSGE AVATTTMT
