TSGA_ECOLI
ID TSGA_ECOLI Reviewed; 393 AA.
AC P60778; P21229; P37612; Q2M730;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Protein TsgA;
GN Name=tsgA; Synonyms=gutS, yhfC, yhfH; OrderedLocusNames=b3364, JW3327;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-107.
RX PubMed=2546924; DOI=10.1128/jb.171.8.4525-4529.1989;
RA Kawamukai M., Matsuda H., Fujii W., Utsumi R., Komano T.;
RT "Nucleotide sequences of fic and fic-1 genes involved in cell filamentation
RT induced by cyclic AMP in Escherichia coli.";
RL J. Bacteriol. 171:4525-4529(1989).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 347-393.
RC STRAIN=K12;
RX PubMed=2543955; DOI=10.1093/nar/17.10.3865;
RA Bell A.I., Gaston K.L., Cole J.A., Busby S.J.W.;
RT "Cloning of binding sequences for the Escherichia coli transcription
RT activators, FNR and CRP: location of bases involved in discrimination
RT between FNR and CRP.";
RL Nucleic Acids Res. 17:3865-3874(1989).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 347-393.
RC STRAIN=K12;
RX PubMed=2200672; DOI=10.1111/j.1432-1033.1990.tb19125.x;
RA Peakman T., Crouzet J., Mayaux J.F., Busby S.J.W., Mohan S., Harborne N.,
RA Wootton J., Nicolson R., Cole J.A.;
RT "Nucleotide sequence, organisation and structural analysis of the products
RT of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome.";
RL Eur. J. Biochem. 191:315-323(1990).
RN [6]
RP INDUCTION.
RX PubMed=11055951; DOI=10.1128/aem.66.11.4972-4978.2000;
RA Guzzo J., Dubow M.S.;
RT "A novel selenite- and tellurite-inducible gene in Escherichia coli.";
RL Appl. Environ. Microbiol. 66:4972-4978(2000).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- INDUCTION: By selenite or tellurite. Maximal expression was detected at
CC concentrations of 10.0 and 0.5 micrograms/ml for sodium selenite and
CC sodium tellurite, respectively. {ECO:0000269|PubMed:11055951}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. TsgA family.
CC {ECO:0000305}.
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DR EMBL; U18997; AAA58161.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76389.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77926.1; -; Genomic_DNA.
DR EMBL; M28363; AAA23771.2; -; Genomic_DNA.
DR EMBL; X14202; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; G65130; G65130.
DR RefSeq; NP_417823.1; NC_000913.3.
DR RefSeq; WP_000185247.1; NZ_STEB01000004.1.
DR AlphaFoldDB; P60778; -.
DR SMR; P60778; -.
DR BioGRID; 4261086; 10.
DR STRING; 511145.b3364; -.
DR TCDB; 2.A.1.7.14; the major facilitator superfamily (mfs).
DR PaxDb; P60778; -.
DR PRIDE; P60778; -.
DR EnsemblBacteria; AAC76389; AAC76389; b3364.
DR EnsemblBacteria; BAE77926; BAE77926; BAE77926.
DR GeneID; 66672755; -.
DR GeneID; 947869; -.
DR KEGG; ecj:JW3327; -.
DR KEGG; eco:b3364; -.
DR PATRIC; fig|1411691.4.peg.3365; -.
DR EchoBASE; EB1242; -.
DR eggNOG; COG0738; Bacteria.
DR HOGENOM; CLU_056916_0_0_6; -.
DR InParanoid; P60778; -.
DR OMA; RFGFVLM; -.
DR PhylomeDB; P60778; -.
DR BioCyc; EcoCyc:YHFC-MON; -.
DR PRO; PR:P60778; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0072714; P:response to selenite ion; IEP:EcoCyc.
DR Gene3D; 1.20.1250.20; -; 2.
DR HAMAP; MF_01044; MFS_TsgA; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR023528; MFS_TsgA.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..393
FT /note="Protein TsgA"
FT /id="PRO_0000206494"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..50
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..77
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 99..100
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 122..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..161
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..205
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 227..244
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..272
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 294..296
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 318..331
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..360
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382..393
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 393 AA; 43166 MW; 75445C6136809691 CRC64;
MTNSNRIKLT WISFLSYALT GALVIVTGMV MGNIADYFNL PVSSMSNTFT FLNAGILISI
FLNAWLMEIV PLKTQLRFGF LLMVLAVAGL MFSHSLALFS AAMFILGVVS GITMSIGTFL
VTQMYEGRQR GSRLLFTDSF FSMAGMIFPM IAAFLLARSI EWYWVYACIG LVYVAIFILT
FGCEFPALGK HAPKTDAPVE KEKWGIGVLF LSVAALCYIL GQLGFISWVP EYAKGLGMSL
NDAGTLVSNF WMSYMVGMWA FSFILRFFDL QRILTVLAGL AAILMYVFNT GTPAHMAWSI
LALGFFSSAI YTTIITLGSQ QTKVPSPKLV NFVLTCGTIG TMLTFVVTGP IVEHSGPQAA
LLTANGLYAV VFVMCFLLGF VSRHRQHNTL TSH
