TSI1_PSEAE
ID TSI1_PSEAE Reviewed; 172 AA.
AC Q9I2Q0;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Immune protein Tsi1 {ECO:0000303|PubMed:22931054};
DE Flags: Precursor;
GN Name=tsi1 {ECO:0000303|PubMed:22931054}; OrderedLocusNames=PA1845;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2] {ECO:0007744|PDB:4EQA}
RP X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 20-172, DISULFIDE BONDS,
RP INTERACTION WITH TSE1, AND FUNCTION.
RX PubMed=22931054; DOI=10.1042/bj20120668;
RA Shang G., Liu X., Lu D., Zhang J., Li N., Zhu C., Liu S., Yu Q., Zhao Y.,
RA Zhang H., Hu J., Cang H., Xu S., Gu L.;
RT "Structural insight into how Pseudomonas aeruginosa peptidoglycanhydrolase
RT Tse1 and its immunity protein Tsi1 function.";
RL Biochem. J. 448:201-211(2012).
RN [3] {ECO:0007744|PDB:3VPJ}
RP X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS), DISULFIDE BONDS, INTERACTION WITH
RP TSE1, AND FUNCTION.
RX PubMed=22700987; DOI=10.1074/jbc.m112.368043;
RA Ding J., Wang W., Feng H., Zhang Y., Wang D.C.;
RT "Structural insights into the Pseudomonas aeruginosa type VI virulence
RT effector Tse1 bacteriolysis and self-protection mechanisms.";
RL J. Biol. Chem. 287:26911-26920(2012).
CC -!- FUNCTION: Immunity protein that plays a role in preventing early
CC activation of toxin Tse1. Binds to a large surface of Tse1 and thereby
CC occludes the active site to specifically inhibits enzyme activity by
CC forming a hydrogen bond with the catalytic diad.
CC {ECO:0000269|PubMed:22700987, ECO:0000269|PubMed:22931054}.
CC -!- SUBUNIT: Forms an heterotetramer with Tse1 consisting of two Tse1
CC dimers and two Tsi1 dimers. Formation of the complex inactivates Tse1
CC enzymatic activity. {ECO:0000269|PubMed:22700987,
CC ECO:0000269|PubMed:22931054}.
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DR EMBL; AE004091; AAG05234.1; -; Genomic_DNA.
DR PIR; G83415; G83415.
DR RefSeq; NP_250536.1; NC_002516.2.
DR RefSeq; WP_003113603.1; NZ_QZGE01000003.1.
DR PDB; 3VPJ; X-ray; 2.50 A; E/F/G/H=1-172.
DR PDB; 4EQA; X-ray; 1.60 A; C/D=20-172.
DR PDB; 4FGI; X-ray; 3.20 A; B/D/F/H=24-172.
DR PDB; 4FQB; X-ray; 2.69 A; B/D/F/H=20-172.
DR PDBsum; 3VPJ; -.
DR PDBsum; 4EQA; -.
DR PDBsum; 4FGI; -.
DR PDBsum; 4FQB; -.
DR AlphaFoldDB; Q9I2Q0; -.
DR SMR; Q9I2Q0; -.
DR PaxDb; Q9I2Q0; -.
DR PRIDE; Q9I2Q0; -.
DR DNASU; 880643; -.
DR EnsemblBacteria; AAG05234; AAG05234; PA1845.
DR GeneID; 880643; -.
DR KEGG; pae:PA1845; -.
DR PATRIC; fig|208964.12.peg.1918; -.
DR PseudoCAP; PA1845; -.
DR HOGENOM; CLU_106704_0_0_6; -.
DR OMA; NDCRIED; -.
DR BioCyc; PAER208964:G1FZ6-1884-MON; -.
DR Proteomes; UP000002438; Chromosome.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..172
FT /note="Immune protein Tsi1"
FT /evidence="ECO:0000255"
FT /id="PRO_5004326982"
FT DISULFID 22..167
FT /evidence="ECO:0007744|PDB:3VPJ, ECO:0007744|PDB:4EQA,
FT ECO:0007744|PDB:4FQB"
FT DISULFID 79..121
FT /evidence="ECO:0007744|PDB:3VPJ, ECO:0007744|PDB:4EQA,
FT ECO:0007744|PDB:4FGI"
FT DISULFID 147..155
FT /evidence="ECO:0007744|PDB:3VPJ, ECO:0007744|PDB:4EQA,
FT ECO:0007744|PDB:4FGI"
FT STRAND 24..26
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 33..35
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 38..42
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 44..51
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 61..63
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 68..72
FT /evidence="ECO:0007829|PDB:4EQA"
FT TURN 73..75
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 78..81
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 87..95
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 98..107
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 110..117
FT /evidence="ECO:0007829|PDB:4EQA"
FT TURN 118..120
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 123..128
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 133..137
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 140..151
FT /evidence="ECO:0007829|PDB:4EQA"
FT HELIX 152..154
FT /evidence="ECO:0007829|PDB:4EQA"
FT STRAND 155..161
FT /evidence="ECO:0007829|PDB:4EQA"
FT HELIX 164..167
FT /evidence="ECO:0007829|PDB:4EQA"
SQ SEQUENCE 172 AA; 18757 MW; 552D398571AADD55 CRC64;
MKLLAGSFAA LFLSLSAQAA DCTFTQLEIV PQFGSPNMFG GEDEHVRVMF SNEDPNDDNP
DAFPEPPVYL ADRDSGNDCR IEDGGIWSRG GVFLSQDGRR VLMHEFSGSS AELVSYDSAT
CKVVHREDIS GQRWAVDKDG LRLGQKCSGE SVDSCAKIVK RSLAPFCQTA KK
