TSI2_PSEAE
ID TSI2_PSEAE Reviewed; 77 AA.
AC Q9I0D9;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Immune protein Tsi2 {ECO:0000303|PubMed:22310046};
DE AltName: Full=Anti-toxin protein Tsi2;
GN Name=tsi2 {ECO:0000303|PubMed:22310046}; OrderedLocusNames=PA2703;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2] {ECO:0007744|PDB:3STQ}
RP X-RAY CRYSTALLOGRAPHY (2.28 ANGSTROMS), SUBUNIT, AND FUNCTION.
RX PubMed=22310046; DOI=10.1016/j.jmb.2012.01.040;
RA Zou T., Yao X., Qin B., Zhang M., Cai L., Shang W., Svergun D.I., Wang M.,
RA Cui S., Jin Q.;
RT "Crystal structure of Pseudomonas aeruginosa Tsi2 reveals a stably folded
RT superhelical antitoxin.";
RL J. Mol. Biol. 417:351-361(2012).
RN [3] {ECO:0007744|PDB:3RQ9}
RP X-RAY CRYSTALLOGRAPHY (1.00 ANGSTROMS), FUNCTION, DISRUPTION PHENOTYPE, AND
RP INTERACTION WITH TSE2.
RX PubMed=22511866; DOI=10.1371/journal.ppat.1002613;
RA Li M., Le Trong I., Carl M.A., Larson E.T., Chou S., De Leon J.A.,
RA Dove S.L., Stenkamp R.E., Mougous J.D.;
RT "Structural basis for type VI secretion effector recognition by a cognate
RT immunity protein.";
RL PLoS Pathog. 8:E1002613-E1002613(2012).
RN [4] {ECO:0007744|PDB:5AKO}
RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS), FUNCTION, AND INTERACTION WITH
RP TSE2.
RX PubMed=26749446; DOI=10.1016/j.str.2015.11.012;
RA Robb C.S., Robb M., Nano F.E., Boraston A.B.;
RT "The Structure of the Toxin and Type Six Secretion System Substrate Tse2 in
RT Complex with Its Immunity Protein.";
RL Structure 24:277-284(2016).
CC -!- FUNCTION: Immunity protein that plays a role in preventing early
CC activation of toxin Tse2. Binds to a large surface of Tse2 and thereby
CC occludes the active site to specifically inhibits Tse2.
CC {ECO:0000269|PubMed:22310046, ECO:0000269|PubMed:22511866,
CC ECO:0000269|PubMed:26749446}.
CC -!- SUBUNIT: Forms an heterotetramer with Tse2 consisting of two Tse2
CC dimers and two Tsi2 dimers. Formation of the complex inactivates Tse2
CC enzymatic activity. {ECO:0000269|PubMed:22310046,
CC ECO:0000269|PubMed:22511866, ECO:0000269|PubMed:26749446}.
CC -!- DISRUPTION PHENOTYPE: Deletion of Tsi2 is lethal in the presence of
CC Tse2. {ECO:0000269|PubMed:22511866}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE004091; AAG06091.1; -; Genomic_DNA.
DR PIR; C83308; C83308.
DR RefSeq; NP_251393.1; NC_002516.2.
DR RefSeq; WP_003114383.1; NZ_QZGE01000011.1.
DR PDB; 3RQ9; X-ray; 1.00 A; A/B=1-77.
DR PDB; 3STQ; X-ray; 2.28 A; A/B/C/D/E/F=1-77.
DR PDB; 3VPV; X-ray; 1.80 A; A/B=1-77.
DR PDB; 4C18; X-ray; 1.49 A; A/B=1-77.
DR PDB; 5AKO; X-ray; 2.40 A; A/B=1-77.
DR PDBsum; 3RQ9; -.
DR PDBsum; 3STQ; -.
DR PDBsum; 3VPV; -.
DR PDBsum; 4C18; -.
DR PDBsum; 5AKO; -.
DR AlphaFoldDB; Q9I0D9; -.
DR SMR; Q9I0D9; -.
DR DIP; DIP-61910N; -.
DR IntAct; Q9I0D9; 1.
DR STRING; 287.DR97_5256; -.
DR PaxDb; Q9I0D9; -.
DR PRIDE; Q9I0D9; -.
DR EnsemblBacteria; AAG06091; AAG06091; PA2703.
DR GeneID; 880324; -.
DR KEGG; pae:PA2703; -.
DR PATRIC; fig|208964.12.peg.2829; -.
DR PseudoCAP; PA2703; -.
DR HOGENOM; CLU_2635258_0_0_6; -.
DR BioCyc; PAER208964:G1FZ6-2743-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR CDD; cd11690; Tsi2_like; 1.
DR InterPro; IPR033783; Tsi2.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Reference proteome.
FT CHAIN 1..77
FT /note="Immune protein Tsi2"
FT /id="PRO_0000449042"
FT DISULFID 14
FT /note="Interchain"
FT /evidence="ECO:0007744|PDB:4C18"
FT STRAND 1..3
FT /evidence="ECO:0007829|PDB:3STQ"
FT HELIX 5..25
FT /evidence="ECO:0007829|PDB:3RQ9"
FT TURN 26..28
FT /evidence="ECO:0007829|PDB:3RQ9"
FT HELIX 31..61
FT /evidence="ECO:0007829|PDB:3RQ9"
FT STRAND 62..64
FT /evidence="ECO:0007829|PDB:5AKO"
FT HELIX 68..71
FT /evidence="ECO:0007829|PDB:3RQ9"
FT HELIX 75..77
FT /evidence="ECO:0007829|PDB:3VPV"
SQ SEQUENCE 77 AA; 8520 MW; 539669B9AD50641B CRC64;
MNLKPQTLMV AIQCVAARTR ELDAQLQNDD PQNAAELEQL LVGYDLAADD LKNAYEQALG
QYSGLPPYDR LIEEPAS
