TSI3_PSEAE
ID TSI3_PSEAE Reviewed; 145 AA.
AC Q9HYC4;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Immune protein Tsi3 {ECO:0000303|PubMed:24025333};
DE AltName: Full=Anti-toxin protein Tsi3;
DE Flags: Precursor;
GN Name=tsi3 {ECO:0000303|PubMed:24025333}; OrderedLocusNames=PA3485;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2] {ECO:0007744|PDB:4LUQ}
RP X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS) OF 23-145 IN COMPLEX WITH CALCIUM,
RP FUNCTION, AND INTERACTION WITH TSE3.
RX PubMed=24025333; DOI=10.1074/jbc.c113.506097;
RA Li L., Zhang W., Liu Q., Gao Y., Gao Y., Wang Y., Wang D.Z., Li Z.,
RA Wang T.;
RT "Structural Insights on the bacteriolytic and self-protection mechanism of
RT muramidase effector Tse3 in Pseudomonas aeruginosa.";
RL J. Biol. Chem. 288:30607-30613(2013).
RN [3] {ECO:0007744|PDB:4M5F, ECO:0007744|PDB:4N7S, ECO:0007744|PDB:4N80}
RP X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 27-145, FUNCTION, AND INTERACTION
RP WITH TSE3.
RX PubMed=24724564; DOI=10.1111/mmi.12616;
RA Lu D., Shang G., Zhang H., Yu Q., Cong X., Yuan J., He F., Zhu C., Zhao Y.,
RA Yin K., Chen Y., Hu J., Zhang X., Yuan Z., Xu S., Hu W., Cang H., Gu L.;
RT "Structural insights into the T6SS effector protein Tse3 and the Tse3-Tsi3
RT complex from Pseudomonas aeruginosa reveal a calcium-dependent membrane-
RT binding mechanism.";
RL Mol. Microbiol. 92:1092-1112(2014).
CC -!- FUNCTION: Immunity protein that plays a role in preventing early
CC activation of toxin Tse3. Occupies Tse3 substrate binding site and
CC prevents the substrate from entering. {ECO:0000269|PubMed:24025333,
CC ECO:0000269|PubMed:24724564}.
CC -!- SUBUNIT: Forms an heterotetramer with Tse3 consisting of two Tse3
CC dimers and two Tsi3 dimers. Formation of the complex inactivates Tse3
CC enzymatic activity. {ECO:0000269|PubMed:24025333,
CC ECO:0000269|PubMed:24724564}.
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DR EMBL; AE004091; AAG06873.1; -; Genomic_DNA.
DR PIR; F83210; F83210.
DR RefSeq; NP_252175.1; NC_002516.2.
DR RefSeq; WP_003113900.1; NZ_QZGE01000039.1.
DR PDB; 3WA5; X-ray; 1.90 A; B=1-145.
DR PDB; 4LUQ; X-ray; 1.77 A; C/D=23-145.
DR PDB; 4M5F; X-ray; 2.50 A; B=21-145.
DR PDB; 4N7S; X-ray; 2.10 A; B/D=27-145.
DR PDB; 4N80; X-ray; 2.40 A; B=23-144.
DR PDB; 4N88; X-ray; 2.80 A; B/D=27-145.
DR PDBsum; 3WA5; -.
DR PDBsum; 4LUQ; -.
DR PDBsum; 4M5F; -.
DR PDBsum; 4N7S; -.
DR PDBsum; 4N80; -.
DR PDBsum; 4N88; -.
DR AlphaFoldDB; Q9HYC4; -.
DR SMR; Q9HYC4; -.
DR STRING; 287.DR97_4436; -.
DR PaxDb; Q9HYC4; -.
DR DNASU; 879938; -.
DR EnsemblBacteria; AAG06873; AAG06873; PA3485.
DR GeneID; 879938; -.
DR KEGG; pae:PA3485; -.
DR PATRIC; fig|208964.12.peg.3648; -.
DR PseudoCAP; PA3485; -.
DR HOGENOM; CLU_1785220_0_0_6; -.
DR OMA; RWIVVSA; -.
DR BioCyc; PAER208964:G1FZ6-3553-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Lipoprotein; Metal-binding; Palmitate;
KW Reference proteome; Signal.
FT SIGNAL 1..15
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 16..145
FT /note="Immune protein Tsi3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT /id="PRO_5004327368"
FT REGION 53..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 126
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0007744|PDB:4LUQ"
FT LIPID 16
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 16
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT STRAND 24..29
FT /evidence="ECO:0007829|PDB:4LUQ"
FT STRAND 33..39
FT /evidence="ECO:0007829|PDB:4LUQ"
FT STRAND 42..46
FT /evidence="ECO:0007829|PDB:4LUQ"
FT STRAND 48..57
FT /evidence="ECO:0007829|PDB:4LUQ"
FT STRAND 64..72
FT /evidence="ECO:0007829|PDB:4LUQ"
FT STRAND 79..83
FT /evidence="ECO:0007829|PDB:4LUQ"
FT STRAND 86..98
FT /evidence="ECO:0007829|PDB:4LUQ"
FT STRAND 101..112
FT /evidence="ECO:0007829|PDB:4LUQ"
FT STRAND 115..128
FT /evidence="ECO:0007829|PDB:4LUQ"
FT HELIX 133..141
FT /evidence="ECO:0007829|PDB:4LUQ"
SQ SEQUENCE 145 AA; 15792 MW; 60222E1FE5E7A1BC CRC64;
MKTVALILAS LALLACTAES GVDFDKTLTH PNGLVVERPV GFDARRSAEG FRFDEGGKLR
NPRQLEVQRQ DAPPPPDLAS RRLGDGEARY KVEEDDGGSA GSEYRLWAAK PAGARWIVVS
ASEQSEDGEP TFALAWALLE RARLQ
