ID   TSI4_PSEAE              Reviewed;         145 AA.
AC   Q9I068;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Immune protein Tsi4 {ECO:0000303|PubMed:24589350};
GN   Name=tsi4 {ECO:0000303|PubMed:24589350}; OrderedLocusNames=PA2775;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=24589350; DOI=10.1111/mmi.12571;
RA   Whitney J.C., Beck C.M., Goo Y.A., Russell A.B., Harding B.N.,
RA   De Leon J.A., Cunningham D.A., Tran B.Q., Low D.A., Goodlett D.R.,
RA   Hayes C.S., Mougous J.D.;
RT   "Genetically distinct pathways guide effector export through the type VI
RT   secretion system.";
RL   Mol. Microbiol. 92:529-542(2014).
CC   -!- FUNCTION: Immunity protein that plays a role in preventing early
CC       activation of toxin Tse4. {ECO:0000269|PubMed:24589350}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant together with deletion of toxin
CC       Tse4 leads to a significant loss of fitness advantage when placed in
CC       competition with parental strains. {ECO:0000269|PubMed:24589350}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE004091; AAG06163.1; -; Genomic_DNA.
DR   PIR; F83298; F83298.
DR   RefSeq; NP_251465.1; NC_002516.2.
DR   RefSeq; WP_003114429.1; NZ_QZGE01000011.1.
DR   AlphaFoldDB; Q9I068; -.
DR   SMR; Q9I068; -.
DR   STRING; 287.DR97_5173; -.
DR   PaxDb; Q9I068; -.
DR   PRIDE; Q9I068; -.
DR   EnsemblBacteria; AAG06163; AAG06163; PA2775.
DR   GeneID; 880541; -.
DR   KEGG; pae:PA2775; -.
DR   PATRIC; fig|208964.12.peg.2913; -.
DR   PseudoCAP; PA2775; -.
DR   HOGENOM; CLU_130398_0_0_6; -.
DR   OMA; GEHVSYP; -.
DR   BioCyc; PAER208964:G1FZ6-2822-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR021994; DUF3592.
DR   Pfam; PF12158; DUF3592; 1.
PE   3: Inferred from homology;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..145
FT                   /note="Immune protein Tsi4"
FT                   /id="PRO_0000449110"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   145 AA;  14992 MW;  D9C7CE689E086995 CRC64;
     MTTSSGSRIG GLLHASLFSL LGLGLLLAGG FKTVERYHFL RTAQEAQGTV SALNAGGSHP
     QIDFTSVSGE RISYPQGGFI FGYQVGEPVR VLYEAGRPAA SAIVDDAGAL WGTGAFLCGF
     GALFGIVGFR GLLGLRSSQP TSKGH