ID   TSI6_PSEAE              Reviewed;          94 AA.
AC   Q9I740;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Immune protein Tsi6 {ECO:0000303|PubMed:26456113};
GN   Name=tsi6 {ECO:0000303|PubMed:26456113}; OrderedLocusNames=PA0092;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2] {ECO:0007744|PDB:4ZUY, ECO:0007744|PDB:4ZV0}
RP   X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS), FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=26456113; DOI=10.1016/j.cell.2015.09.027;
RA   Whitney J.C., Quentin D., Sawai S., LeRoux M., Harding B.N., Ledvina H.E.,
RA   Tran B.Q., Robinson H., Goo Y.A., Goodlett D.R., Raunser S., Mougous J.D.;
RT   "An interbacterial NAD(P)(+) glycohydrolase toxin requires elongation
RT   factor Tu for delivery to target cells.";
RL   Cell 163:607-619(2015).
RN   [3] {ECO:0007744|PDB:5JTK}
RP   STRUCTURE BY NMR.
RA   Barnwal R.P., Varani G.;
RT   "NMR structure of Uncharacterized protein from Pseudomonas aeruginosa
RT   PAO1.";
RL   Submitted (MAY-2016) to the PDB data bank.
CC   -!- FUNCTION: Immunity protein that plays a role in preventing early
CC       activation of toxin Tse6. {ECO:0000269|PubMed:26456113}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant cells show Tse6-dependent
CC       intoxication with a dramatic increase in division time.
CC       {ECO:0000269|PubMed:26456113}.
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DR   EMBL; AE004091; AAG03482.1; -; Genomic_DNA.
DR   PIR; A83633; A83633.
DR   RefSeq; NP_248782.1; NC_002516.2.
DR   RefSeq; WP_003104961.1; NZ_QZGE01000015.1.
DR   PDB; 4ZUY; X-ray; 1.95 A; A/B=1-94.
DR   PDB; 4ZV0; X-ray; 1.40 A; B=1-94.
DR   PDB; 5JTK; NMR; -; A=1-94.
DR   PDBsum; 4ZUY; -.
DR   PDBsum; 4ZV0; -.
DR   PDBsum; 5JTK; -.
DR   AlphaFoldDB; Q9I740; -.
DR   BMRB; Q9I740; -.
DR   SMR; Q9I740; -.
DR   STRING; 287.DR97_3049; -.
DR   PaxDb; Q9I740; -.
DR   PRIDE; Q9I740; -.
DR   EnsemblBacteria; AAG03482; AAG03482; PA0092.
DR   GeneID; 880664; -.
DR   KEGG; pae:PA0092; -.
DR   PATRIC; fig|208964.12.peg.96; -.
DR   PseudoCAP; PA0092; -.
DR   HOGENOM; CLU_161899_0_0_6; -.
DR   OMA; LKDAHYV; -.
DR   BioCyc; PAER208964:G1FZ6-94-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0097351; F:toxin sequestering activity; IMP:PseudoCAP.
DR   GO; GO:0033103; P:protein secretion by the type VI secretion system; IMP:PseudoCAP.
DR   InterPro; IPR040818; Tsi6.
DR   Pfam; PF18660; Tsi6; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome.
FT   CHAIN           1..94
FT                   /note="Immune protein Tsi6"
FT                   /id="PRO_0000449112"
FT   HELIX           3..20
FT                   /evidence="ECO:0007829|PDB:4ZV0"
FT   HELIX           25..42
FT                   /evidence="ECO:0007829|PDB:4ZV0"
FT   STRAND          44..46
FT                   /evidence="ECO:0007829|PDB:5JTK"
FT   HELIX           49..53
FT                   /evidence="ECO:0007829|PDB:4ZV0"
FT   HELIX           56..63
FT                   /evidence="ECO:0007829|PDB:4ZV0"
FT   TURN            65..67
FT                   /evidence="ECO:0007829|PDB:4ZV0"
FT   HELIX           69..86
FT                   /evidence="ECO:0007829|PDB:4ZV0"
SQ   SEQUENCE   94 AA;  10678 MW;  4C8929784FFD6352 CRC64;
     MTPIEYIDRA LALVVDRLAR YPGYEVLLSA EKQLQYIRSV LLDRSLDRSA LHRLTLGSIA
     VKEFDETDPE LSRALKDAYY VGIRTGRGLK VDLP