ID   TSIV2_VIBCH             Reviewed;         242 AA.
AC   Q9KNE4;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Immunity protein TsiV2 {ECO:0000303|PubMed:24348240};
GN   Name=tsiV2 {ECO:0000303|PubMed:24348240}; OrderedLocusNames=VC_A0021;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24348240; DOI=10.1371/journal.ppat.1003752;
RA   Miyata S.T., Unterweger D., Rudko S.P., Pukatzki S.;
RT   "Dual expression profile of type VI secretion system immunity genes
RT   protects pandemic Vibrio cholerae.";
RL   PLoS Pathog. 9:E1003752-E1003752(2013).
CC   -!- FUNCTION: Immunity protein that plays a role in preventing early
CC       activation of toxin VasX. {ECO:0000269|PubMed:24348240}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant is susceptible to killing by V52
CC       strain in a VasX-dependent manner. {ECO:0000269|PubMed:24348240}.
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DR   EMBL; AE003853; AAF95935.1; -; Genomic_DNA.
DR   PIR; A82512; A82512.
DR   RefSeq; NP_232422.1; NC_002506.1.
DR   RefSeq; WP_000904079.1; NZ_LT906615.1.
DR   AlphaFoldDB; Q9KNE4; -.
DR   STRING; 243277.VC_A0021; -.
DR   TCDB; 1.C.131.1.1; the vasx toxin (vasx) family.
DR   DNASU; 2612522; -.
DR   EnsemblBacteria; AAF95935; AAF95935; VC_A0021.
DR   GeneID; 57741496; -.
DR   KEGG; vch:VC_A0021; -.
DR   PATRIC; fig|243277.26.peg.2668; -.
DR   eggNOG; ENOG502ZEUJ; Bacteria.
DR   HOGENOM; CLU_100876_0_0_6; -.
DR   OMA; PEMAYKV; -.
DR   BioCyc; VCHO:VCA0021-MON; -.
DR   Proteomes; UP000000584; Chromosome 2.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Host membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..242
FT                   /note="Immunity protein TsiV2"
FT                   /id="PRO_0000449208"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   242 AA;  27881 MW;  3A15742D69821146 CRC64;
     MLIDKNELEQ LKVKLHSSEV IYQWDSVAYG ERRSEIFRVF GAISAGIVPL WPFIFFADIQ
     FNSKEFWGFI CFSLAGMAAA RYLFMPDHRY CYSLTQAGIY YTDQEVIPDA AYTFVRGFAW
     VGIAVCLLAL AVVGPLAFVG AGGFALLAFG LTNFHPTVHK KEVYFADQLI VFDPIKEKMV
     DLNTDSTDEP WFDRRLFFSS LDEKTHFIEL VKSIHNNVDY LPLQRVNDQY KHPIFNQELK
     EE